Effects of carbonate and phosphate concentrations on hydrogen production using anaerobic sewage sludge microflora

Abstract Anaerobic sewage sludge acclimated with sucrose in a completely stirred tank reactor operating at 35°C was used as the seed (dominated by Clostridia species) in a batch experiment to investigate the concentration effects of carbonate and phosphate on biological hydrogen production. The Taguchi orthogonal array was used in the experimental design and a confirmation experiment was conducted. Experimental results indicated that the hydrogen production ability of the anaerobic microflora in sewage sludge was affected by NH4HCO3, Na2HPO4 and Na2CO3 with Na2HPO4 being the most significant supplement. Na2HPO4 affected the hydrogen production in a concentration-dependent way with 600 mg/l the optimal concentration. The hydrogen production rate was 1.9 times enhanced, compared with an acidogenic nutrient formulation (Proc. Soc. Civ. Engrs. 325 (1982) 61), when a proper carbonate and phosphate concentration formulation was used. The enhancement was due to a shortening of the microflora lag-time. Based on the enhancement abilities on hydrogen content and hydrogen production rate, adding phosphate instead of carbonate as a buffering capacity supplement might be a useful strategy for optimal hydrogen production operations with anaerobic sewage cultures.

[1]  Tatsushi Kawai,et al.  Biological production of hydrogen from cellulose by natural anaerobic microflora , 1995 .

[2]  R. J. Zoetemeyer,et al.  Influence of temperature on the anaerobic acidification of glucose in a mixed culture forming part of a two-stage digestion process , 1982 .

[3]  J. Zeikus,et al.  Regulation of hydrogen metabolism in Butyribacterium methylotrophicum by substrate and pH , 1996, Applied Microbiology and Biotechnology.

[4]  C-C. Chen,et al.  Kinetics of hydrogen production with continuous anaerobic cultures utilizing sucrose as the limiting substrate , 2001, Applied Microbiology and Biotechnology.

[5]  D. L. Hawkes,et al.  Sustainable fermentative hydrogen production: challenges for process optimisation , 2002 .

[6]  J. Lay,et al.  Biohydrogen production as a function of pH and substrate concentration. , 2001, Environmental science & technology.

[7]  Effect of rapid temperature change and HRT on anaerobic acidogenesis , 1997 .

[8]  Hubert Bahl,et al.  Parameters Affecting Solvent Production by Clostridium pasteurianum , 1992, Applied and environmental microbiology.

[9]  Chiu-Yue Lin,et al.  Hydrogen production during the anaerobic acidogenic conversion of glucose , 1999 .

[10]  Chiu-Yue Lin,et al.  Carbon/nitrogen-ratio effect on fermentative hydrogen production by mixed microflora , 2004 .

[11]  Grietje Zeeman,et al.  Anaerobic sewage treatment , 2005 .

[12]  Debabrata Das,et al.  Hydrogen production by biological processes: a survey of literature , 2001 .

[13]  J. Lay,et al.  Feasibility of biological hydrogen production from organic fraction of municipal solid waste , 1999 .

[14]  J. Matsumoto,et al.  Fundamental Studies on Hydrogen Production in the Acid-Forming Phase and Its Bacteria in Anaerobic Treatment Processes – The Effects of Solids Retention Time , 1993 .

[15]  Akiko Miya,et al.  Studies on hydrogen production by continuous culture system of hydrogen-producing anaerobic bacteria , 1997 .

[16]  J N Lester,et al.  Anaerobic acidogenesis of a complex wastewater: I. The influence of operational parameters on reactor performance , 1988, Biotechnology and bioengineering.

[17]  J. Lay,et al.  Modeling and optimization of anaerobic digested sludge converting starch to hydrogen , 2000, Biotechnology and bioengineering.

[18]  I. Lundström,et al.  Hydrogen production from organic waste , 2001 .

[19]  H. H. Fang,et al.  Acidification of mid- and high-strength dairy wastewaters. , 2001, Water research.

[20]  P. Mccarty,et al.  Environmental Biotechnology: Principles and Applications , 2000 .

[21]  Y. Ueno,et al.  Hydrogen Production from Industrial Wastewater by Anaerobic Microflora in Chemostat Culture , 1996 .

[22]  Akashah Majizat,et al.  Hydrogen gas production from glucose and its microbial kinetics in anaerobic systems , 1997 .