Effective therapy for a murine model of human anaplastic large-cell lymphoma with the anti-CD30 monoclonal antibody, HeFi-1, does not require activating Fc receptors.

CD30 is a member of the tumor necrosis factor receptor family. Overexpression of CD30 on some neoplasms versus its limited expression on normal tissues makes this receptor a promising target for antibody-based therapy. Anaplastic large-cell lymphoma (ALCL) represents a heterogeneous group of aggressive non-Hodgkin lymphomas characterized by the strong expression of CD30. We investigated the therapeutic efficacy of HeFi-1, a mouse IgG1 monoclonal antibody, which recognizes the ligand-binding site on CD30, and humanized anti-Tac antibody (daclizumab), which recognizes CD25, in a murine model of human ALCL. The ALCL model was established by intravenous injection of karpas299 cells into nonobese diabetic/severe combined immuno-deficient (SCID/NOD) wild-type or SCID/NOD Fc receptor common gamma chain-deficient (FcRgamma(-/-)) mice. HeFi-1, given at a dose of 100 microg weekly for 4 weeks, significantly prolonged survival of the ALCL-bearing SCID/NOD wild-type and SCID/NOD FcRgamma(-/-) mice (P < .01) as compared with the control groups. In vitro studies showed that HeFi-1 inhibited the proliferation of karpas299 cells, whereas daclizumab did not inhibit cell proliferation. We demonstrated that the expression of FcRgamma on polymorphonuclear leukocytes and monocytes was not required for HeFi-1-mediated tumor growth inhibition in vivo, although it was required for daclizumab.

[1]  P. Bruhns,et al.  FcγRIV : A novel FcR with distinct IgG subclass specificity , 2005 .

[2]  P. Bruhns,et al.  FcgammaRIV: a novel FcR with distinct IgG subclass specificity. , 2005, Immunity.

[3]  W. Wilson,et al.  Elevated serum-soluble interleukin-2 receptor levels in patients with anaplastic large cell lymphoma. , 2004, Blood.

[4]  T. Waldmann,et al.  Activating Fc Receptors Are Required for Antitumor Efficacy of the Antibodies Directed toward CD25 in a Murine Model of Adult T-Cell Leukemia , 2004, Cancer Research.

[5]  H. Merz,et al.  Signal Transduction in Anaplastic Large Cell Lymphoma Cells (ALCL) Mediated by the Tumor Necrosis Factor Receptor CD30 , 2004, Leukemia & lymphoma.

[6]  T. Waldmann,et al.  Effective therapy for a murine model of adult T-cell leukemia with the humanized anti-CD52 monoclonal antibody, Campath-1H. , 2003, Cancer research.

[7]  T. Waldmann,et al.  Effective therapy for a murine model of adult T-cell leukemia with the humanized anti-CD2 monoclonal antibody, MEDI-507. , 2003, Blood.

[8]  T. Waldmann,et al.  Immunotherapy: past, present and future , 2003, Nature Medicine.

[9]  Trisha Gura,et al.  Therapeutic antibodies: Magic bullets hit the target , 2002, Nature.

[10]  C. Schneider,et al.  Pleiotropic Signal Transduction Mediated by Human CD30: A Member of the Tumor Necrosis Factor Receptor (TNFR) Family , 2002, Leukemia & lymphoma.

[11]  P. Carter,et al.  Improving the efficacy of antibody-based cancer therapies , 2001, Nature Reviews Cancer.

[12]  E. Hildt,et al.  CD30-mediated cell cycle arrest associated with induced expression of p21CIP1/WAF1 in the anaplastic large cell lymphoma cell line Karpas 299 , 2001, Oncogene.

[13]  C. Duckett,et al.  Differential effects of CD30 activation in anaplastic large cell lymphoma and Hodgkin disease cells. , 2000, Blood.

[14]  S. Pileri,et al.  CD30(+) anaplastic large cell lymphoma: a review of its histopathologic, genetic, and clinical features. , 2000, Blood.

[15]  R. Junghans,et al.  Anti-CD30 antibody–based therapy , 2000, Current opinion in oncology.

[16]  S. Pileri,et al.  Expression of the CD30 antigen in non‐lymphoid tissues and cells , 2000, The Journal of pathology.

[17]  L. Presta,et al.  Inhibitory Fc receptors modulate in vivo cytoxicity against tumor targets , 2000, Nature Medicine.

[18]  M. Kadin,et al.  A murine xenograft model for human CD30+ anaplastic large cell lymphoma. Successful growth inhibition with an anti-CD30 antibody (HeFi-1). , 1999, The American journal of pathology.

[19]  R. Warnke,et al.  CD30 expression is common in mediastinal large B-cell lymphoma. , 1999, American journal of clinical pathology.

[20]  V. Diehl,et al.  An anti-CD30 single-chain Fv selected by phage display and fused to Pseudomonas exotoxin A (Ki-4(scFv)-ETÁ) is a potent immunotoxin against a Hodgkin-derived cell line , 1999, British Journal of Cancer.

[21]  R. Levy,et al.  Rituximab chimeric anti-CD20 monoclonal antibody therapy for relapsed indolent lymphoma: half of patients respond to a four-dose treatment program. , 1998, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[22]  A. Houghton,et al.  Fc receptors are required in passive and active immunity to melanoma. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[23]  C. Thompson,et al.  CD30-dependent degradation of TRAF2: implications for negative regulation of TRAF signaling and the control of cell survival. , 1997, Genes & development.

[24]  L. Polito,et al.  Anti‐CD30 (BER‐H2) immunotoxins containing the type‐1 ribosome‐inactivating proteins momordin and PAP‐S (pokeweed antiviral protein from seeds) display powerful antitumour activity against CD30+ tumour cells in vitro and in SCID mice , 1996, British journal of haematology.

[25]  T. Waldmann,et al.  Radioimmunotherapy of interleukin-2R alpha-expressing adult T-cell leukemia with Yttrium-90-labeled anti-Tac. , 1995, Blood.

[26]  D. Longo,et al.  In vivo antitumor effects of unconjugated CD30 monoclonal antibodies on human anaplastic large-cell lymphoma xenografts. , 1995, Cancer research.

[27]  R. Willemze,et al.  CD30 expression in normal and neoplastic lymphoid tissue: biological aspects and clinical implications. , 1995, Leukemia.

[28]  I. Bernstein,et al.  Phase II trial of 131I-B1 (anti-CD20) antibody therapy with autologous stem cell transplantation for relapsed B cell lymphomas , 1995, The Lancet.

[29]  L. Polito,et al.  Antitumor activity of anti-CD30 immunotoxin (Ber-H2/saporin) in vitro and in severe combined immunodeficiency disease mice xenografted with human CD30+ anaplastic large-cell lymphoma. , 1995, Blood.

[30]  H. Stein,et al.  CD30 antigen in embryonal carcinoma and embryogenesis and release of the soluble molecule. , 1995, The American journal of pathology.

[31]  P. Rudolph,et al.  Expression of CD 30 and nerve growth factor‐receptor in neoplastic and reactive vascular lesions: an immunohistochemical study , 1993, Histopathology.

[32]  I. Pastan,et al.  Preclinical evaluation of 111In-labeled B3 monoclonal antibody: biodistribution and imaging studies in nude mice bearing human epidermoid carcinoma xenografts. , 1993, Cancer research.

[33]  M. Kadin Lymphomatoid papulosis, Ki-1+ lymphoma, and primary cutaneous Hodgkin's disease. , 1991, Seminars in dermatology.

[34]  I. Pastan,et al.  Characterization of monoclonal antibodies B1 and B3 that react with mucinous adenocarcinomas. , 1991, Cancer research.

[35]  S. Mirzadeh,et al.  Radiometal labeling of immunoproteins: covalent linkage of 2-(4-isothiocyanatobenzyl)diethylenetriaminepentaacetic acid ligands to immunoglobulin. , 1990, Bioconjugate chemistry.

[36]  T. Waldmann,et al.  A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells. , 1981, Journal of immunology.

[37]  T. Waldmann,et al.  A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. II. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells. , 1981, Journal of immunology.