Emerging Technologies for Mycotoxin Detection

The history of the development of analytical methods for detecting fungal toxins is rich and varied. Method development has followed a process somewhat akin to Darwinian evolution: methods are selected based upon the characteristics most desirable to the analyst. Typically, this has lead to the development of accurate and sensitive methods for their detection, with a recurring emphasis on improving the speed and lowering the costs of the assays. Like evolution, there have been radical developments, incremental developments, and techniques that have fallen from favor only to be rediscovered. This review focuses on recent developments in technologies for detection of mycotoxins, with a particular emphasis on the myriad forms of biosensors that have begun to appear. Specifically, recent development in evanescent wave technologies (surface plasmon resonance, fiber optic sensors), lateral flow and dipstick devices, fluorescence polarization and time‐resolved fluorescence, microbead assays, and capillary electrophoretic immunoassays, are described. The challenge for the emerging technologies is to demonstrate advantages over the more conventional, and better established, techniques in settings outside the analytical laboratory.

[1]  Mary W Trucksess,et al.  Electrospray mass spectrometry for fumonisin detection and method validation. , 2002, Advances in experimental medicine and biology.

[2]  R. B. Sashidhar,et al.  Use of ion-exchange chromatography coupled with TLC-laser scanning densitometry for the quantitation of fumonisin B1 , 1999 .

[3]  U. Narang,et al.  Multianalyte detection using a capillary-based flow immunosensor. , 1998, Analytical biochemistry.

[4]  Robert M. Carter,et al.  A Fluorescent Biosensor for Detection of Zearalenone , 2000 .

[5]  C Van Peteghem,et al.  Flow-through membrane-based enzyme immunoassay for rapid detection of ochratoxin A in wheat. , 1999, Journal of food protection.

[6]  Ewald Usleber,et al.  Rapid Detection of Fumonisin B1 in Corn-Based Food by Competitive Direct Dipstick Enzyme Immunoassay/Enzyme-Linked Immunofiltration Assay with Integrated Negative Control Reaction , 1995 .

[7]  N. Strachan,et al.  Application of an automated particle‐based immunosensor for the detection of aflatoxin B1 in foods , 1997 .

[8]  Vicki S. Thompson,et al.  Fiber-Optic Immunosensor for the Detection of Fumonisin B1 , 1996 .

[9]  Liberty Sibanda,et al.  A collaborative study to validate novel field immunoassay kits for rapid mycotoxin detection. , 2002, International journal of food microbiology.

[10]  M. Braunagel,et al.  Selection and characterisation of recombinant single-chain antibodies to the hapten Aflatoxin-B1 from naive recombinant antibody libraries. , 2001, Journal of immunological methods.

[11]  S. He,et al.  Expression of a Functional Antizearalenone Single-Chain Fv Antibody in Transgenic ArabidopsisPlants , 2000, Applied and Environmental Microbiology.

[12]  Gordon S. Shephard,et al.  Chromatographic and allied methods of analysis for selected mycotoxins , 1996 .

[13]  Chris M. Maragos,et al.  Detection of the mycotoxin fumonisin B1 by a combination of immunofluorescence and capillary electrophoresis , 1997 .

[14]  S. Garden,et al.  Novel colorimetric immunoassay for the detection of aflatoxin B1 , 2001 .

[15]  B. Liedberg,et al.  Biosensing with surface plasmon resonance--how it all started. , 1995, Biosensors & bioelectronics.

[16]  P Zöllner,et al.  Determination of zearalenone in grains by high-performance liquid chromatography-tandem mass spectrometry after solid-phase extraction with RP-18 columns or immunoaffinity columns. , 1999, Journal of chromatography. A.

[17]  C T Elliott,et al.  Development and validation of dry reagent time-resolved fluoroimmunoassays for zeranol and α -zearalenol to assist in distinguishing zeranol abuse from Fusarium spp. toxin contamination in bovine urine , 2002, Food additives and contaminants.

[18]  S. Hirano,et al.  Near Infra Red Detection of Internally Moldy Nuts. , 1998, Bioscience, biotechnology, and biochemistry.

[19]  George G. Guilbault,et al.  Rapid Detection of Aflatoxin B1 with Immunochemical Optrodes , 1997 .

[20]  R. Krska,et al.  Improving methods of analysis for mycotoxins: molecularly imprinted polymers for deoxynivalenol and zearalenone , 2003, Food additives and contaminants.

[21]  W. Checovich,et al.  Fluorescence polarization — a new tool for cell and molecular biology , 1995, Nature.

[22]  Vicki S. Thompson,et al.  Fiber-optic immunosensor for mycotoxins. , 1999, Natural toxins.

[23]  J B Shear,et al.  Determination of biological toxins using capillary electrokinetic chromatography with multiphoton-excited fluorescence. , 2000, Analytical chemistry.

[24]  R. Tsao,et al.  Micellar electrokinetic capillary electrophoresis for rapid analysis of patulin in apple cider. , 2000, Journal of agricultural and food chemistry.

[25]  D. Bhattacharya,et al.  A novel signal amplification technology for ELISA based on catalyzed reporter deposition. Demonstration of its applicability for measuring aflatoxin B(1). , 1999, Journal of immunological methods.

[26]  M. Dovis,et al.  Determination of aflatoxin B1 in agricultural commodities by time-resolved fluoroimmunoassay and immunoenzymometric assay. , 1994, The Analyst.

[27]  Rudolf Krska,et al.  Fourier transform mid-infrared spectroscopy with attenuated total reflection (FT-IR/ATR) as a tool for the detection of Fusarium fungi on maize , 2002 .

[28]  Christina G. Siontorou,et al.  Rapid methods for detection of Aflatoxin M1 based on electrochemical transduction by self-assembled metal-supported bilayer lipid membranes (s-BLMs) and on interferences with transduction of DNA hybridization , 1998 .

[29]  Richard O'Kennedy,et al.  Production and Characterization of Murine Single Chain Fv Antibodies to Aflatoxin B 1 Derived From a Pre-immunized Antibody Phage Display Library System , 2002 .

[30]  C. V. Van Peteghem,et al.  Dipstick enzyme immunoassay to detect Fusarium T-2 toxin in wheat , 1996, Applied and environmental microbiology.

[31]  Toshitsugu Tanaka,et al.  An application of liquid chromatography and mass spectrometry for determination of aflatoxins , 2002 .

[32]  Wolfgang Lindner,et al.  Towards ochratoxin A selective molecularly imprinted polymers for solid-phase extraction. , 2002, Journal of chromatography. A.

[33]  M. Morgan,et al.  Development of surface plasmon resonance-based immunoassay for aflatoxin B(1). , 2000, Journal of agricultural and food chemistry.

[34]  Frances S. Ligler,et al.  Chemistry and Technology of Evanescent Wave Biosensors , 1991 .

[35]  C. Maragos,et al.  Fluorescence polarization as a tool for the determination of deoxynivalenol in wheat , 2002, Food additives and contaminants.

[36]  Floyd E. Dowell,et al.  Reflectance and Transmittance Spectroscopy Applied to Detecting Fumonisin in Single Corn Kernels Infected with Fusarium verticillioides , 2002 .

[37]  A. Dobson,et al.  PCR-based detection and quantification of mycotoxigenic fungi , 2002 .

[38]  R. Dietrich,et al.  Multimycotoxin dipstick enzyme immunoassay applied to wheat. , 1995, Food additives and contaminants.

[39]  Achille Cappiello,et al.  Determination of aflatoxins in peanut meal by LC/MS with a particle beam interface , 1995 .

[40]  N. Magan,et al.  Detection and differentiation between mycotoxigenic and non‐mycotoxigenic strains of two Fusarium spp. using volatile production profiles and hydrolytic enzymes , 2000, Journal of applied microbiology.

[41]  Tom C. Pearson Machine Vision System for Automated Detection of Stained Pistachio Nuts , 1996 .

[42]  C. Maragos,et al.  Fluorescence polarization as a means for determination of fumonisins in maize. , 2001, Journal of agricultural and food chemistry.

[43]  J Stroka,et al.  Development of a simplified densitometer for the determination of aflatoxins by thin-layer chromatography. , 2000, Journal of chromatography. A.

[44]  E. Stigter,et al.  Rapid surface plasmon resonance-based inhibition assay of deoxynivalenol. , 2003, Journal of agricultural and food chemistry.

[45]  E. Lai,et al.  Immunoassay of fumonisins by a surface plasmon resonance biosensor. , 1998, Analytical biochemistry.

[46]  R. Vogel,et al.  A Biosensor-based Immunoassay for Rapid Screening of Deoxynivalenol Contamination in Wheat , 2002 .

[47]  Chris M Maragos,et al.  Determination of deoxynivalenol and nivalenol in corn and wheat by liquid chromatography with electrospray mass spectrometry. , 2003, Journal of AOAC International.

[48]  Heidi R. C. Dietrich,et al.  Biosensors and multiple mycotoxin analysis , 2003 .

[49]  Paul Chen,et al.  Estimation of Fusarium scab in wheat using machine vision and a neural network , 1998 .

[50]  W. F. McClure,et al.  Dual-wavelength fiber optic photometer measures fluorescence of aflatoxin contaminated pistachio nuts. , 1980 .

[51]  H. W. Ko,et al.  An automated, handheld biosensor for aflatoxin. , 2000, Biosensors & bioelectronics.

[52]  M Valcárcel,et al.  Screening of aflatoxins in feed samples using a flow system coupled to capillary electrophoresis. , 2002, Journal of chromatography. A.

[53]  F. Dowell,et al.  DETECTING AFLATOXIN IN SINGLE CORN KERNELS BY TRANSMITTANCE AND REFLECTANCE SPECTROSCOPY , 2001 .

[54]  Floyd E. Dowell,et al.  Predicting Scab, Vomitoxin, and Ergosterol in Single Wheat Kernels Using Near-Infrared Spectroscopy , 1999 .

[55]  D. Nikolelis,et al.  Flow injection monitoring of aflatoxin M1 in milk and milk preparations using filter-supported bilayer lipid membranes. , 1998, Analytical chemistry.

[56]  M. Hartl,et al.  Simultaneous determination of fumonisin B(1) and hydrolyzed fumonisin B(1) in corn products by liquid chromatography/electrospray ionization mass spectrometry. , 1999, Journal of agricultural and food chemistry.

[57]  J. Schnürer,et al.  Volatiles for mycological quality grading of barley grains: determinations using gas chromatography-mass spectrometry and electronic nose. , 2000, International journal of food microbiology.

[58]  J. Ho,et al.  Detection of fumonisin B1: comparison of flow-injection liposome immunoanalysis with high-performance liquid chromatography. , 2003, Analytical biochemistry.

[59]  C. Maragos,et al.  Fluorescence Polarization Immunoassay of Mycotoxins: A Review , 2009, Toxins.

[60]  James J. Pestka,et al.  Molecular Cloning and Expression of Recombinant Phage Antibody against Fumonisin B1. , 1996, Journal of food protection.

[61]  Michael E Jolley,et al.  Fluorescence polarization (FP) assays for the determination of grain mycotoxins (fumonisins, DON vomitoxin and aflatoxins). , 2003, Combinatorial chemistry & high throughput screening.

[62]  J Dickens,et al.  Modular separation-based fiber-optic sensors for remote in situ monitoring. , 2000, Journal of environmental monitoring : JEM.

[63]  J. Schnürer,et al.  Detection and quantification of ochratoxin A and deoxynivalenol in barley grains by GC-MS and electronic nose. , 2002, International journal of food microbiology.

[64]  P. Scott,et al.  Application of immunoaffinity columns to mycotoxin analysis. , 1997, Journal of AOAC International.

[65]  Chris A. Rowe-Taitt,et al.  Array biosensor for detection of biohazards. , 2000, Biosensors & bioelectronics.

[66]  J. Ho,et al.  A strip liposome immunoassay for aflatoxin B1. , 2002, Analytical chemistry.