Effect of pectin methyl esterase (PME) and CaCl2 infusion on the cell integrity of fresh-cut and frozen-thawed mangoes: An NMR relaxometry study

Abstract This study describes the use of Nuclear Magnetic Resonance (NMR) relaxometry to understand the effect of pectin methyl esterase (PME) + CaCl2 infusion under different conditions on cell integrity of fresh-cut and frozen–thawed mangoes. Infusion experiments were performed at: atmospheric pressure, vacuum conditions of 50 kPa and 10 kPa. For NMR relaxometry experiments T 2 (spin–spin relaxation time), T 1 (spin–lattice-relaxation times) and 2D T 1 –T 2 experiments were performed. Results showed that, as the severity of the vacuum treatment increased, the relaxation times changed significantly (p  1 –T 2 relaxation maps showed that compartmentalization was retained after vacuum treatment even for frozen–thawed samples. The study showed that NMR relaxometry is a useful tool to analyze the cell integrity of mangoes exposed to different treatments.

[1]  Nicole Fassbinder.  Magnetic Resonance Imaging In Food Science , 2016 .

[2]  Alper Sönmez.  An Analysis of the World Market for Tobacco Production and International Trade and Its Importance for Turkey , 2014 .

[3]  B. Hills,et al.  Spatially resolved multidimensional cross-correlation relaxometry. , 2010, Magnetic resonance imaging.

[4]  B. Hills,et al.  Magnetic resonance imaging in food science , 1998 .

[5]  Kirk D. Dolan,et al.  Antioxidant properties, total phenolics, and quality of fresh-cut 'Tommy Atkins' mangoes as affected by different pre-treatments , 2013 .

[6]  M. Hendrickx,et al.  Enzyme infusion and thermal processing of strawberries: Pectin conversions related to firmness evolution , 2009 .

[7]  Brian P. Hills,et al.  NMR studies of changes in subcellular water compartmentation in parenchyma apple tissue during drying and freezing , 1997 .

[8]  M. McCarthy,et al.  Effect of controlled atmosphere storage on pomegranate quality investigated by two dimensional NMR correlation spectroscopy , 2013 .

[9]  Moshe Rosenberg,et al.  Magnetic resonance imaging (MRI) and relaxation spectrum analysis as methods to investigate swelling in whey protein gels. , 2010, Journal of food science.

[10]  Henk Van As,et al.  Intact plant MRI for the study of cell water relations, membrane permeability, cell-to-cell and long distance water transport. , 2006, Journal of experimental botany.

[11]  Maja Musse,et al.  An investigation of the structural aspects of the tomato fruit by means of quantitative nuclear magnetic resonance imaging. , 2009, Magnetic resonance imaging.

[12]  Henk Van As,et al.  Intact plant MRI for the study of cell water relations, membrane permeability, cell-to-cell and long distance water transport. , 2007, Journal of experimental botany.

[13]  F. Capozzi,et al.  Magnetic resonance in food science – meeting the challenge , 2011, Magnetic resonance in chemistry : MRC.

[14]  W. Boonsupthip,et al.  Utilization of partially ripe mangoes for freezing preservation by impregnation of mango juice and sugars , 2011 .

[15]  C. Fyfe,et al.  Investigation of Hydrogel Formation from Hydroxypropylmethylcellulose (HPMC) by NMR Spectroscopy and NMR Imaging Techniques , 1997 .

[16]  W. Bradley,et al.  MRI: The Basics , 1997 .

[17]  Lu Zhang,et al.  Black heart characterization and detection in pomegranate using NMR relaxometry and MR imaging , 2012 .

[18]  Brian P. Hills,et al.  Two-dimensional NMR relaxation studies of apple quality , 2008 .

[19]  S. Jurga,et al.  1 H NMR and Rheological Studies of the Calcium Induced Gelation Process in Aqueous Low Methoxyl Pectin Solutions , 2005 .

[20]  P. Van Cutsem,et al.  Pectin methylesterases induce an abrupt increase of acidic pectin during strawberry fruit ripening. , 2008, Journal of plant physiology.

[21]  E. Morris,et al.  Biological interactions between polysaccharides and divalent cations: The egg‐box model , 1973 .

[22]  Guylaine Collewet,et al.  Quantitative MRI in Food Science & Food Engineering , 2012 .

[23]  K. Arndt,et al.  Investigation of swelling and diffusion in polymers by 1H NMR imaging: LCP networks and hydrogels , 2000 .

[24]  Keith W. Waldron,et al.  Characterisation of Ripening and Pressure-Induced Changes in Tomato Pericarp Using NMR Relaxometry , 2009 .

[25]  M. Hendrickx,et al.  Enzyme infusion prior to thermal/high pressure processing of strawberries: Mechanistic insight into firmness evolution , 2010 .

[26]  M. Devaux,et al.  Changes in texture, cellular structure and cell wall composition in apple tissue as a result of freezing , 2009 .

[27]  Paul J. Kramer,et al.  Water Relations of Plants , 1983 .

[28]  B. Hills,et al.  NMR relaxation study of avocado quality , 2005 .

[29]  N. Hernández-Sánchez,et al.  An NMR study on internal browning in pears , 2007 .

[30]  U. Lüttge.  PLANT CELL MEMBRANES AND SALINITY: STRUCTURAL, BIOCHEMICAL AND BIOPHYSICAL CHANGES , 1993 .

[31]  Pietro Rocculi,et al.  Modification of Transverse NMR Relaxation Times and Water Diffusion Coefficients of Kiwifruit Pericarp Tissue Subjected to Osmotic Dehydration , 2013, Food and Bioprocess Technology.

[32]  Antonio Raffo,et al.  Ripening of banana fruit monitored by water relaxation and diffusion 1H-NMR measurements , 2005 .

[33]  B. Hills,et al.  T1−T2 NMR correlation studies of high-pressure-processed starch and potato tissue , 2005 .

[34]  W. Kerr,et al.  NMR proton relaxation measurements of water associated with high methoxy and low methoxy pectins , 2000 .

[35]  Aaron R. Uesugi.  Pulsed Light Treatment , 2010 .

[36]  D. Black,et al.  Desiccation and Survival in Plants : Drying without Dying , 2005 .

[37]  M. Devaux,et al.  Firming of fruit tissues by vacuum-infusion of pectin methylesterase: Visualisation of enzyme action. , 2008, Food chemistry.

[38]  Mecit Halil Oztop,et al.  Monitoring changes in feta cheese during brining by magnetic resonance imaging and NMR relaxometry , 2011 .

[39]  Florence Charles,et al.  Pulsed light treatment as new method to maintain physical and nutritional quality of fresh-cut mangoes , 2013 .

[40]  M. Oppen,et al.  An Analysis of the World Market for Mangos and its Importance for Developing Countries 1 , 2003 .

[41]  C. J. Clark,et al.  Quality Assessment of Horticultural Products by NMR , 2003 .

[42]  I. Lara,et al.  Cell wall-modifying enzymes and firmness loss in ripening ‘Golden Reinders’ apples: A comparison between calcium dips and ULO storage , 2011 .

[43]  S. Pinheiro,et al.  Modulation of tomato pericarp firmness through pH and calcium: Implications for the texture of fresh-cut fruit , 2008 .

[44]  M. McCarthy,et al.  PhysicalProperties Disintegration Efficiency of Pulsed Electric Field Induced Effects on Onion ( Allium cepa L . ) Tissues as a Function of Pulse Protocol and Determination of Cell Integrity by 1 H-NMR Relaxometry , 2010 .

[45]  D. Barrett,et al.  Texture improvement of fresh and frozen mangoes with pectin methylesterase and calcium infusion. , 2012, Journal of the science of food and agriculture.

[46]  B. Hills,et al.  T1-T2 correlation analysis of complex foods , 2004 .

[47]  H. Van As,et al.  Probing water compartments and membrane permeability in plant cells by H NMR relaxation measurements. , 1992, Biophysical journal.

[48]  Maja Musse,et al.  Monitoring the postharvest ripening of tomato fruit using quantitative MRI and NMR relaxometry. , 2009 .