Fluorescence immunoassay of anti-cyclic citrulinated peptide (CCP) autoantibodies by using parylene-H film

A fluorescence immunoassay for rheumatoid arthritis was presented by immobilizing a short peptide cyclic citrullinated peptide (CCP) through parylene-H film. The parylene-H film is a polymer of pxylene modified with formyl groups which can react with primary amine groups of proteins or peptides. In this work, the covalent coupling of the peptide can be produced by only one step of incubation without additional coupling reagent, and the immobilization efficiency to the parylene-H film was compared with the immobilization of CCP through conventional physical adsorption. The applicability of this immobilization method for short peptides is demonstrated by detecting autoantibodies in rheumatoid arthritis patient serum.

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