Ethanol production from pretreated wheat straw hydrolyzate by Saccharomyces cerevisiae via sequential statistical optimization.

Abstract Wheat straw is one of the most abundant lignocellulosic feedstocks, contains mixture of sugars, selected for ethanol production. Microwave alkali pretreated wheat straw was used as substrate for ethanol production from Saccharomyces cerevisiae . Optimization of ethanol production from wheat straw enzymatic hydrolyzate was studied using sequential statistical optimization process. First factors affecting ethanol production were screened out by Plackett–Burman design (PBD). Most significant factors pH (4–7), temperature (20–40 °C), initial total reducing sugar conc. (TRS) (1–10%) and inoculum level (1–5%) further studied by Box–Behnken design (BBD) for ethanol production. Maximum ethanol production obtained during BBD was 15.6 g/L with ethanol productivities 0.43 g/L/h. Under optimum conditions ethanol production studied at bioreactor level and obtained ethanol concentration 16.4 g/L with ethanol productivities 0.45 g/L/h obtained at pH 5.5, temperature 30 °C, inoculums level 3.3% and TRS conc. 6.5%. These results indicated that ethanol production can be enhanced by optimization of fermentation conditions.

[1]  J. N. Nigam.  Continuous ethanol production from pineapple cannery waste using immobilized yeast cells. , 2000, Journal of biotechnology.

[2]  Elmer Ccopa Rivera,et al.  Evaluation of optimization techniques for parameter estimation: Application to ethanol fermentation considering the effect of temperature , 2006 .

[3]  Tomas Brandberg,et al.  Comparison of vacuum and high pressure evaporated wood hydrolyzate for enthanol production by repeated fed-batch using flocculating Saccharomyces cerevisiae , 2009, BioResources.

[4]  N. Bishnoi,et al.  Optimization of ethanol production from microwave alkali pretreated rice straw using statistical experimental designs by Saccharomyces cerevisiae , 2012 .

[5]  J Olley,et al.  Predictive microbiology: towards the interface and beyond. , 2002, International journal of food microbiology.

[6]  M. Elibol,et al.  Optimization of medium composition for actinorhodin production by Streptomyces coelicolor A3(2) with response surface methodology , 2004 .

[7]  Paul Christakopoulos,et al.  An alternative approach to the bioconversion of sweet sorghum carbohydrates to ethanol , 1995 .

[8]  Qunhui Wang,et al.  ETHANOL PRODUCTION FROM KITCHEN GARBAGE USING RESPONSE SURFACE METHODOLOGY , 2008 .

[9]  A. Gómez-Puyou,et al.  Effect of the pH of the incubation medium on glycolysis and respiration in Saccharomyces cerevisiae. , 1972, Archives of biochemistry and biophysics.

[10]  Yanli Chen.  Development and application of co-culture for ethanol production by co-fermentation of glucose and xylose: a systematic review , 2011, Journal of Industrial Microbiology & Biotechnology.

[11]  J. Laukevics,et al.  The effect of osmo-induced stress on product formation by Zymomonas mobilis on sucrose. , 2000, International journal of food microbiology.

[12]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[13]  L. Xia,et al.  Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate , 2007 .

[14]  Douglas C. Montgomery,et al.  Response Surface Methodology: Process and Product Optimization Using Designed Experiments , 1995 .

[15]  M. Ueda,et al.  Spectrophotometric Determination of Ethanol in Wine , 1968, American Journal of Enology and Viticulture.

[16]  Anita Singh,et al.  Enzymatic hydrolysis optimization of microwave alkali pretreated wheat straw and ethanol production by yeast. , 2012, Bioresource technology.

[17]  F. Kargı,et al.  Effects of feed sugar concentration on continuous ethanol fermentation of cheese whey powder solution (CWP). , 2007 .

[18]  Wiwut Tanthapanichakoon,et al.  Mathematical modeling to investigate temperature effect on kinetic parameters of ethanol fermentation , 2006 .

[19]  Rajeev K Sukumaran,et al.  Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production , 2009 .

[20]  Namita Singh,et al.  Enhanced saccharification of rice straw and hull by microwave-alkali pretreatment and lignocellulolytic enzyme production. , 2011, Bioresource technology.

[21]  Irini Angelidaki,et al.  Production of bioethanol from wheat straw: An overview on pretreatment, hydrolysis and fermentation. , 2010, Bioresource technology.

[22]  Lisbeth Olsson,et al.  Fueling industrial biotechnology growth with bioethanol. , 2007, Advances in biochemical engineering/biotechnology.

[23]  Deniz Cekmecelioglu,et al.  Cost-effective approach to ethanol production and optimization by response surface methodology. , 2011, Waste management.

[24]  Inés Joekes,et al.  Ethanol fermentation of a diluted molasses medium by Saccharomyces cerevisiae immobilized on chrysotile , 2003 .

[25]  L. Olsson,et al.  Comparison of SHF and SSF processes from steam‐exploded wheat straw for ethanol production by xylose‐fermenting and robust glucose‐fermenting Saccharomyces cerevisiae strains , 2008, Biotechnology and bioengineering.

[26]  A. Tahir,et al.  EFFECT OF CULTURAL CONDITIONS ON ETHANOL PRODUCTION BY LOCALLY ISOLATED SACCHAROMYCES CEREVISIAE BIO-07 , 2010 .

[27]  Reeta Rani Singhania,et al.  Bio-ethanol from water hyacinth biomass: an evaluation of enzymatic saccharification strategy. , 2010, Bioresource technology.