Analysis of electrophoretically heterogeneous lipopolysaccharides of Escherichia coli by immunoblotting

The purified lipopolysaccharide (LPS) from each of four Escherichia coli serogroups which was separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and visualized by periodic acid-silver staining, exhibited a different distribution of bands. The same serogroup-specific banding patterns were evident on immunoblots developed with homologous but not heterologous O-antisera. Thus, the electrophoretic heterogeneity of LPS was confirmed and the multiple bands seen on SDS-PAGE further shown to contain O-serogroup-specific antigenic determinants.

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