论文信息 - Statistical accuracy in fluorescence fluctuation experiments

Statistical accuracy in fluorescence fluctuation experiments

Abstract The mathematical expression of the signal to noise ratio in fluorescence fluctuation experiments is derived for arbitrary sample profiles and for any mechanism of translational motion, and experimentally verified. The signal to noise ratio depends on the mean count rate per particle per dwell time, the mean number of particles per sample volume, time characteristics of the correlation function, sample profile characteristics, and the data collection time. Statistical accuracy of the third order moment of fluorescence intensity fluctuations is also studied. The optimum concentration for the third order moment analysis is about one particle per sample volume.

[1] E. Elson,et al. Fluorescence correlation spectroscopy. I. Conceptual basis and theory , 1974 .

[2] W. Webb,et al. Fluorescence correlation spectroscopy. II. An experimental realization , 1974, Biopolymers.

[3] H. Qian,et al. Distribution of molecular aggregation by analysis of fluctuation moments. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[4] H. Qian,et al. On the statistics of fluorescence correlation spectroscopy. , 1990, Biophysical chemistry.

[5] H. Qian,et al. On the analysis of high order moments of fluorescence fluctuations. , 1990, Biophysical journal.

[6] Dennis E. Koppel,et al. Statistical accuracy in fluorescence correlation spectroscopy , 1974 .