Electrospun Polycaprolactone (PCL) Degradation: An In Vitro and In Vivo Study

Polycaprolactone (PCL) is widely used in tissue engineering due to its interesting properties, namely biocompatibility, biodegradability, elastic nature, availability, cost efficacy, and the approval of health authorities such as the American Food and Drug Administration (FDA). The PCL degradation rate is not the most adequate for specific applications such as skin regeneration due to the hydrophobic nature of bulk PCL. However, PCL electrospun fiber meshes, due to their low diameters resulting in high surface area, are expected to exhibit a fast degradation rate. In this work, in vitro and in vivo degradation studies were performed over 90 days to evaluate the potential of electrospun PCL as a wound dressing. Enzymatic and hydrolytic degradation studies in vitro, performed in a static medium, demonstrated the influence of lipase, which promoted a rate of degradation of 97% for PCL meshes. In an in vivo scenario, the degradation was slower, although the samples were not rejected, and were well-integrated in the surrounding tissues inside the subcutaneous pockets specifically created.

[1]  J. R. Dias,et al.  Multifunctional Gelatin/Chitosan Electrospun Wound Dressing Dopped with Undaria pinnatifida Phlorotannin-Enriched Extract for Skin Regeneration , 2021, Pharmaceutics.

[2]  M. Nourani,et al.  Effects of bilayer nanofibrillar scaffolds containing epidermal growth factor on full‐thickness wound healing , 2020 .

[3]  R. Siegel,et al.  In vitro and in vivo studies of biaxially electrospun poly(caprolactone)/gelatin nanofibers, reinforced with cellulose nanocrystals, for wound healing applications , 2020, Cellulose.

[4]  P. Bártolo,et al.  Biomechanical performance of hybrid electrospun structures for skin regeneration. , 2018, Materials science & engineering. C, Materials for biological applications.

[5]  Deng-Guang Yu,et al.  Fast dissolving drug delivery membrane based on the ultra‐thin shell of electrospun core‐shell nanofibers , 2018, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[6]  Xiangyu Jin,et al.  An electrospun poly(ε-caprolactone) nanocomposite fibrous mat with a high content of hydroxyapatite to promote cell infiltration , 2018, RSC advances.

[7]  X. Qian,et al.  Engineering anisotropic biphasic Janus-type polymer nanofiber scaffold networks via centrifugal jet spinning. , 2017, Journal of biomedical materials research. Part B, Applied biomaterials.

[8]  P. Bártolo,et al.  In situ crosslinked electrospun gelatin nanofibers for skin regeneration , 2017 .

[9]  P. Bártolo,et al.  Advances in electrospun skin substitutes , 2016 .

[10]  Daniel J. Modulevsky,et al.  Biocompatibility of Subcutaneously Implanted Plant-Derived Cellulose Biomaterials , 2016, bioRxiv.

[11]  P. Tengvall,et al.  Foreign Body Reaction to Biomaterials: On Mechanisms for Buildup and Breakdown of Osseointegration. , 2016, Clinical implant dentistry and related research.

[12]  Liqun Zhang,et al.  Fabrication and evaluation of a homogeneous electrospun PCL-gelatin hybrid membrane as an anti-adhesion barrier for craniectomy. , 2015, Journal of materials chemistry. B.

[13]  Ning-hua Liu,et al.  Preparation and Characterization of Electrospun PLCL/Poloxamer Nanofibers and Dextran/Gelatin Hydrogels for Skin Tissue Engineering , 2014, PloS one.

[14]  A. Crawford,et al.  Fabrication and evaluation of electrospun PCL-gelatin micro-/nanofiber membranes for anti-infective GTR implants. , 2014, Journal of materials chemistry. B.

[15]  Masami Okamoto,et al.  Synthetic biopolymer nanocomposites for tissue engineering scaffolds , 2013 .

[16]  A. Gnanamani,et al.  Electrospinning of type I collagen and PCL nanofibers using acetic acid , 2012 .

[17]  B. Marí,et al.  Hydrolytic and enzymatic degradation of a poly(ε-caprolactone) network , 2012 .

[18]  Tao Wang,et al.  Hydrogel sheets of chitosan, honey and gelatin as burn wound dressings , 2012 .

[19]  V. B. Konkimalla,et al.  Poly-є-caprolactone based formulations for drug delivery and tissue engineering: A review. , 2012, Journal of controlled release : official journal of the Controlled Release Society.

[20]  T. Maekawa,et al.  POLYMERIC SCAFFOLDS IN TISSUE ENGINEERING APPLICATION: A REVIEW , 2011 .

[21]  Dietmar W. Hutmacher,et al.  Design, fabrication and characterization of PCL electrospun scaffolds—a review , 2011 .

[22]  Cato T Laurencin,et al.  Biomedical Applications of Biodegradable Polymers. , 2011, Journal of polymer science. Part B, Polymer physics.

[23]  F. O'Brien Biomaterials & scaffolds for tissue engineering , 2011 .

[24]  S. Downes,et al.  Physicochemical characterisation of degrading polycaprolactone scaffolds , 2010 .

[25]  Donald V. Rosato,et al.  Plastics Technology Handbook , 2010 .

[26]  L. Mattoso,et al.  Structural, Electrical, Mechanical, and Thermal Properties of Electrospun Poly(lactic acid)/Polyaniline Blend Fibers , 2010 .

[27]  Shih-Jung Liu,et al.  Electrospun PLGA/collagen nanofibrous membrane as early-stage wound dressing , 2010 .

[28]  Hui Peng,et al.  Controlled enzymatic degradation of poly(ɛ-caprolactone)-based copolymers in the presence of porcine pancreatic lipase , 2010 .

[29]  R L Reis,et al.  Starch–poly(ε‐caprolactone) and starch–poly(lactic acid) fibre‐mesh scaffolds for bone tissue engineering applications: structure, mechanical properties and degradation behaviour , 2008, Journal of tissue engineering and regenerative medicine.

[30]  Andreas Greiner,et al.  Electrospinning: a fascinating method for the preparation of ultrathin fibers. , 2007, Angewandte Chemie.

[31]  B. Bay,et al.  Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. , 2007, Acta biomaterialia.

[32]  J. Sarasua,et al.  Crystallization, morphology, and mechanical behavior of polylactide/poly(ε‐caprolactone) blends , 2006 .

[33]  S. Ramakrishna,et al.  Fabrication of collagen-coated biodegradable polymer nanofiber mesh and its potential for endothelial cells growth. , 2005, Biomaterials.

[34]  N. Bölgen,et al.  In vitro and in vivo degradation of non-woven materials made of poly(ε-caprolactone) nanofibers prepared by electrospinning under different conditions , 2005 .

[35]  M. Kotaki,et al.  A review on polymer nanofibers by electrospinning and their applications in nanocomposites , 2003 .

[36]  Francisco M Gama,et al.  In vitro assessment of the enzymatic degradation of several starch based biomaterials. , 2003, Biomacromolecules.

[37]  K. Shakesheff,et al.  Polymeric systems for controlled drug release. , 1999, Chemical reviews.

[38]  C. L. Y. Leon,et al.  New perspectives in mercury porosimetry , 1998 .

[39]  N. Tietz,et al.  Lipase in serum--the elusive enzyme: an overview. , 1993, Clinical chemistry.

[40]  V. Crescenzi,et al.  Thermodynamics of fusion of poly-β-propiolactone and poly-ϵ-caprolactone. comparative analysis of the melting of aliphatic polylactone and polyester chains , 1972 .

[41]  L. H. Jansen,et al.  Some mechanical properties of human abdominal skin measured on excised strips: a study of their dependence on age and how they are influenced by the presence of striae. , 1958, Dermatologica.

[42]  J. Tao,et al.  The tissue response and degradation of electrospun poly( ε -caprolactone)/poly(trimethylene-carbonate) scaffold in subcutaneous space of mice , 2014 .

[43]  M. Natu,et al.  Influence of polymer processing technique on long term degradation of poly(ε-caprolactone) constructs , 2013 .

[44]  Paulo Jorge Da Silva bartolo,et al.  Characterisation of PCL and PCL/PLA Scaffolds for Tissue Engineering☆ , 2013 .

[45]  Tarun Garg,et al.  Scaffold: a novel carrier for cell and drug delivery. , 2012, Critical reviews in therapeutic drug carrier systems.

[46]  J. L. Escobar Ivirico,et al.  Alkaline degradation study of linear and network poly(ε-caprolactone) , 2011, Journal of materials science. Materials in medicine.

[47]  M. Coret,et al.  Methodology to determine failure characteristics of planar soft tissues using a dynamic tensile test. , 2007, Journal of biomechanics.

[48]  H. S. Azevedo,et al.  Understanding the enzymatic degradation of biodegradable polymers and strategies to control their degradation rate , 2005 .

[49]  Lorna J. Gibson,et al.  Cellular materials as porous scaffolds for tissue engineering , 2001 .

[50]  H. G. Vogel,et al.  Age dependence of mechanical and biochemical properties of human skin. I: Stress-strain experiments, skin thickness and biochemical analysis , 1987 .