In vivo detection of reduced scattering coefficient of C6 glioma in rat brain tissue by near-infrared spectroscopy.

The purpose of this study is to investigate the reduced scattering coefficient of C6 glioma by the near-infrared (NIR) technique. Light scattering properties of C6 glioma in brain tissue is measured by NIR spectroscopy within the wavelength range from 700 to 850 nm. C6 gliomas were implanted in rats' right brains. The scattering properties of the left and right target corresponding to the position of normal and tumor tissue were measured by a bifurcated needle probe on postoperative days 3, 10, and 17. The results show that there was no significant difference in reduced scattering coefficient between left and right brain tissue at postoperative day 3, but significant decreases were found between left and right brains at postoperative days 10 and 17. This study proved our initial hypothesis that the NIR technique may have a potential for clinical application in brain muglioma diagnosis.

[1]  A. Welch,et al.  A review of the optical properties of biological tissues , 1990 .

[2]  C Sato,et al.  Exchange transfusion with fluorocarbon for studying synaptically evoked optical signal in rat cortex. , 2000, Brain research. Brain research protocols.

[3]  S. Terakawa,et al.  Nuclear disintegration as a leading step of glutamate excitotoxicity in brain neurons , 1996, Journal of neuroscience research.

[4]  R. Gupta,et al.  Analysis of changes in energy and redox states in HepG2 hepatoma and C6 glioma cells upon exposure to cadmium. , 2004, Toxicology.

[5]  R. Andrew,et al.  Potential sources of intrinsic optical signals imaged in live brain slices. , 1999, Methods.

[6]  H. Sostman,et al.  Effects of therapy on the 1H NMR spectrum of a human glioma line. , 1994, Magnetic resonance imaging.

[7]  Francis A. Duck,et al.  Mechanical Properties of Tissue , 1990 .

[8]  P. Marquet,et al.  In vivo local determination of tissue optical properties: applications to human brain. , 1999, Applied optics.

[9]  J Beuthan,et al.  The spatial variation of the refractive index in biological cells. , 1996, Physics in medicine and biology.

[10]  Hanli Liu,et al.  Validation of a near-infrared probe for detection of thin intracranial white matter structures. , 2003, Journal of neurosurgery.

[11]  Gerhard J. Mueller,et al.  Correlation of intrahepatic light and temperature distribution in laser-induced thermotherapy of liver tumors and liver tissue , 1998, European Conference on Biomedical Optics.

[12]  H. Liu,et al.  Computational and in vivo investigation of optical reflectance from human brain to assist neurosurgery. , 1998, Journal of biomedical optics.

[13]  B. Kristal,et al.  Mitochondrial Permeability Transition in the Central Nervous System: Induction by Calcium Cycling‐Dependent and ‐Independent Pathways , 1997, Journal of neurochemistry.

[14]  M. Palkovits,et al.  A brain-tumor model utilizing stereotactic implantation of a permanent cannula. , 1993, Journal of Neurosurgery.

[15]  John R. Cameron,et al.  Physical Properties of Tissue. A Comprehensive Reference Book, edited by Francis A. Duck , 1991 .

[16]  B. Feuerstein,et al.  Molecular cytogenetic analysis of chromosomes 1 and 19 in glioma cell lines. , 2005, Cancer genetics and cytogenetics.

[17]  P. Saint-Pierre,et al.  Optimal times for constrained nonlinear control problems without local controllability , 1997 .

[18]  Francis A. Duck,et al.  Physical properties of tissue : a comprehensive reference book , 1990 .

[19]  I J Bigio,et al.  Spectroscopic diagnosis of bladder cancer with elastic light scattering , 1995, Lasers in surgery and medicine.

[20]  S C Gebhart,et al.  In vitro determination of normal and neoplastic human brain tissue optical properties using inverse adding-doubling , 2006, Physics in medicine and biology.

[21]  Mamoru Tamura,et al.  Anoxia induces matrix shrinkage accompanied by an increase in light scattering in isolated brain mitochondria , 2004, Brain Research.

[22]  Hanli Liu,et al.  Look-Ahead Distance of a fiber probe used to assist neurosurgery: Phantom and Monte Carlo study. , 2003, Optics express.

[23]  M. Moller,et al.  Analysis of time-domain optical mammograms recorded from more than 150 patients , 2005, SPIE BiOS.

[24]  P. Vrignaud,et al.  Assessment of the experimental model of transplanted C6 glioblastoma in wistar rats , 1989, Journal of Neuro-Oncology.

[25]  Frédéric Bevilacqua.  Local optical characterization of biological tissues in vitro and in vivo , 1998 .

[26]  J. Tonn,et al.  Spontaneous regression of experimental gliomas—An immunohistochemical and MRI study of the C6 glioma spheroid implantation model , 2004, Experimental Neurology.

[27]  T. Kitai,et al.  Contribution of the mitochondrial compartment to the optical properties of the rat liver: a theoretical and practical approach. , 1994, Biophysical journal.

[28]  B. Xiao,et al.  An experimental study of dendritic cells-mediated immunotherapy against intracranial gliomas in rats , 2005, Journal of Neuro-Oncology.

[29]  D. Richter,et al.  Intrinsic optical signals in respiratory brain stem regions of mice: neurotransmitters, neuromodulators, and metabolic stress. , 2001, Journal of neurophysiology.

[30]  R Richards-Kortum,et al.  Reflectance spectroscopy with polarized light: is it sensitive to cellular and nuclear morphology. , 1999, Optics express.

[31]  B. Beauvoit,et al.  Correlation between the light scattering and the mitochondrial content of normal tissues and transplantable rodent tumors. , 1995, Analytical biochemistry.

[32]  D A Turner,et al.  Use of intrinsic optical signals to monitor physiological changes in brain tissue slices. , 1999, Methods.

[33]  Nitish V Thakor,et al.  Optical light scatter imaging of cellular and sub-cellular morphology changes in stressed rat hippocampal slices , 2000, Journal of Neuroscience Methods.

[34]  H. Liu,et al.  Use of an intracranial near-infrared probe for localization during stereotactic surgery for movement disorders. , 2000, Journal of neurosurgery.

[35]  S Andersson-Engels,et al.  Changes in spectral shape of tissue optical properties in conjunction with laser-induced thermotherapy. , 1998, Applied optics.

[36]  Bruce J. Tromberg,et al.  Superficial tissue optical property determination using spatially resolved measurements close to the source: comparison with frequency-domain photon migration measurements , 1999, Photonics West - Biomedical Optics.

[37]  G. Zonios,et al.  Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo. , 1999, Applied optics.

[38]  M. Schweiger,et al.  Theoretical and experimental investigation of near-infrared light propagation in a model of the adult head. , 1997, Applied optics.

[39]  Harry L. Graber,et al.  Mapping of photon distribution and imaging of MR-derived anatomically accurate optical models of the female breast , 1995, Photonics West.

[40]  Andre Roggan,et al.  Optical properties of adenocarcinoma and squamous cell carcinoma of the gastroesophageal junction. , 2007, Journal of biomedical optics.

[41]  Henry Hirschberg,et al.  Determination of fluence rate and temperature distributions in the rat brain; implications for photodynamic therapy. , 2007, Journal of biomedical optics.

[42]  Bert Grobben,et al.  Rat C6 glioma as experimental model system for the study of glioblastoma growth and invasion , 2002, Cell and Tissue Research.

[43]  S L Jacques,et al.  In vivo determination of optical properties of normal and tumor tissue with white light reflectance and an empirical light transport model during endoscopy. , 2005, Journal of biomedical optics.