Automated microfluidic compact disc (CD) cultivation system of Caenorhabditis elegans

Microfabricated platforms for automated cultivation and behavioral observation of animals would be a valuable tool in physiological and genetic studies. We report an automated microfluidic compact disc (CD) system specialized for cultivating and monitoring Caenorhabditis elegans, proficient in automated feeding, waste removal and live-animal microscopy. The automated CD cultivation platform contains cultivation, nutrient, and waste chamber, channels connecting the chambers, and venting holes. The feeding and waste removal processes are achieved automatically using centrifugal force driven fluidics. Compared to other microfluidic technologies for moving small amounts of fluidic or suspended particles from site to site, this centrifuge-based system is well suited for functions such as flow sequencing, mixing, capillary measuring, and switching. Those functions can be implemented by exploiting centrifugal, coriolis and capillary forces combined with specific microfluidic network. Thus, the CD cultivating system has the capacity to incorporate diverse molecular, biochemical, and pharmacological assays for real-time physiological and behavioral tests of live-animals. This compact and fully automated microfabricated platform is designed to address the unique requirements of space biology due to the stringent limitations on power, size, weight, and crew intervention.

[1]  Norman F. Sheppard,et al.  Centrifugal Microfluidics: Applications , 2000 .

[2]  Beibei Zhao,et al.  Reversal Frequency in Caenorhabditis elegans Represents an Integrated Response to the State of the Animal and Its Environment , 2003, The Journal of Neuroscience.

[3]  H. Horvitz,et al.  The GABAergic nervous system of Caenorhabditis elegans , 1993, Nature.

[4]  R. Wassersug,et al.  Amphibian development in the virtual absence of gravity. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[5]  É. Le Bourg.  A review of the effects of microgravity and of hypergravity on aging and longevity. , 1999, Experimental gerontology.

[6]  G M Whitesides,et al.  Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping. , 2000, Analytical chemistry.

[7]  J. P. Schroeter,et al.  The effects of increased gravity and microgravity on cardiac morphology. , 1998, Aviation, space, and environmental medicine.

[8]  S. Rattan.  Aging intervention, prevention, and therapy through hormesis. , 2004, The journals of gerontology. Series A, Biological sciences and medical sciences.

[9]  S. Brenner.  The genetics of Caenorhabditis elegans. , 1974, Genetics.

[10]  Hongkai Wu,et al.  Fabrication of complex three-dimensional microchannel systems in PDMS. , 2003, Journal of the American Chemical Society.

[11]  Siegfried Hekimi,et al.  Genetics and the Specificity of the Aging Process , 2003, Science.

[12]  Rajesh Ranganathan,et al.  C. elegans Locomotory Rate Is Modulated by the Environment through a Dopaminergic Pathway and by Experience through a Serotonergic Pathway , 2000, Neuron.

[13]  A. Alfonso,et al.  Alternative splicing leads to two cholinergic proteins in Caenorhabditis elegans. , 1994, Journal of molecular biology.

[14]  W. Wood.  The Nematode Caenorhabditis elegans , 1988 .

[15]  L. Qiu,et al.  Energy metabolism and its relation to survival and infectivity of infective juveniles of Steinernema carpocapsae under aerobic conditions , 2000 .

[16]  D. Hall,et al.  Stochastic and genetic factors influence tissue-specific decline in ageing C. elegans , 2002, Nature.

[17]  G. Whitesides,et al.  Soft lithography in biology and biochemistry. , 2001, Annual review of biomedical engineering.

[18]  A. V. Maricq,et al.  Dopamine and Glutamate Control Area-Restricted Search Behavior in Caenorhabditis elegans , 2004, The Journal of Neuroscience.

[19]  M. Tatar,et al.  The Endocrine Regulation of Aging by Insulin-like Signals , 2003, Science.

[20]  Nathaniel J Szewczyk,et al.  Caenorhabditis elegans survives atmospheric breakup of STS-107, space shuttle Columbia. , 2005, Astrobiology.

[21]  B. Merz.  The body pays a penalty for defying the law of gravity. , 1986, Journal of the American Medical Association (JAMA).

[22]  S. Brenner,et al.  The structure of the nervous system of the nematode Caenorhabditis elegans. , 1986, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[23]  A. V. Maricq,et al.  Neuronal Control of Locomotion in C. elegans Is Modified by a Dominant Mutation in the GLR-1 Ionotropic Glutamate Receptor , 1999, Neuron.

[24]  D. Tomko,et al.  Effect of gravity on vestibular neural development , 1998, Brain Research Reviews.

[25]  Marc J. Madou,et al.  Centrifuge-based fluidic platforms , 2004, Proceedings of the IEEE.

[26]  Gregory T. A. Kovacs,et al.  A microfluidic shadow imaging system for the study of the nematode Caenorhabditis elegans in space , 2005 .

[27]  Nathaniel J Szewczyk,et al.  Chemically defined medium and Caenorhabditis elegans , 2003, BMC biotechnology.