Temporal and Tissue-Specific Regulation of a Brassica napus Stearoyl-Acyl Carrier Protein Desaturase Gene

The nucleotide sequence of a Brassica napus stearoyl-acyl carrier protein desaturase gene (Bn10) is presented. This gene is one member of a family of four closely related genes expressed in oilseed rape. The expression of the promoter of this gene in transgenic tobacco was found to be temporally regulated in the developing seed tissues. However, the promoter was also particularly active in other oleogenic tissues such as the tapetum and pollen grains. This raises the interesting question of whether seed-expressed lipid synthesis genes are regulated by separate tissue-specific determinants or by a single factor common to all oleogenic tissues. Parts of the plants undergoing rapid development such as the components of immature flowers and seedlings also exhibited high levels of promoter activity. These tissues are likely to have an elevated requirement for membrane lipid synthesis. Stearoyl-acyl carrier protein desaturase transcript levels have previously been shown to be temporally regulated in the B. napus embryo (S.P. Slocombe, I. Cummins, R.P. Jarvis, D.J. Murphy [1992] Plant Mol Biol 20: 151–155). Evidence is presented demonstrating the induction of desaturase mRNA by abscisic acid in the embryo.

[1]  R. Schilperoort,et al.  Plant Molecular Biology Manual , 1989, Springer Netherlands.

[2]  V. Knauf,et al.  Nucleotide Sequence of a Complementary DNA Clone Encoding Stearoyl-Acyl Carrier Protein Desaturase from Simmondsia chinensis. , 1992, Plant physiology.

[3]  G. Murphy,et al.  Speeding-up the sequencing of double-stranded DNA. , 1988, Nucleic acids research.

[4]  J. Abian,et al.  Effect of abscisic Acid on the linoleic Acid metabolism in developing maize embryos. , 1991, Plant physiology.

[5]  W. Schaffner,et al.  A purine-rich DNA sequence motif present in SV40 and lymphotropic papovavirus binds a lymphoid-specific factor and contributes to enhancer activity in lymphoid cells. , 1987, Genes & development.

[6]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[7]  H F Kern,et al.  Selection of AUG initiation codons differs in plants and animals. , 1987, The EMBO journal.

[8]  A. J. Delisle,et al.  Seed Storage Protein Transcription and mRNA Levels in Brassica napus during Development and in Response to Exogenous Abscisic Acid. , 1989, Plant physiology.

[9]  M. Tombs,et al.  Induction purification and characterization of NADH-specific enoyl acyl carrier protein reductase from developing seeds of oil seed rape (Brassica napus) , 1986 .

[10]  V. Knauf,et al.  Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[11]  H. Davies,et al.  The Primary Structure of a cDNA Clone of the Stearoyl-Acyl Carrier Protein Desaturase Gene from Potato (Solanum tuberosum L.). , 1992, Plant physiology.

[12]  R. Hull,et al.  Transcription of cauliflower mosaic virus DNA. Detection of transcripts, properties, and location of the gene encoding the virus inclusion body protein. , 1981, Virology.

[13]  P. Benfey,et al.  The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants , 1990, Science.

[14]  D. Shintani,et al.  Primary structures of the precursor and mature forms of stearoyl-acyl carrier protein desaturase from safflower embryos and requirement of ferredoxin for enzyme activity. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[15]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[16]  R. Cortese,et al.  Structure and expression of the human haptoglobin locus. , 1985, The EMBO journal.

[17]  C P Joshi,et al.  An inspection of the domain between putative TATA box and translation start site in 79 plant genes. , 1987, Nucleic acids research.

[18]  J. Schussler,et al.  Abscisic Acid and its relationship to seed filling in soybeans. , 1984, Plant physiology.

[19]  C. James,et al.  Plastid-localised seed acyl-carrier protein of Brassica napus is encoded by a distinct, nuclear multigene family. , 1988, European journal of biochemistry.

[20]  R. Foster,et al.  Sequences flanking the hexameric G-box core CACGTG affect the specificity of protein binding. , 1992, The Plant cell.

[21]  J. Ohlrogge,et al.  The genetics of plant lipids. , 1991, Biochimica et biophysica acta.

[22]  R. Weselake,et al.  Developmental Profile of Diacylglycerol Acyltransferase in Maturing Seeds of Oilseed Rape and Safflower and Microspore-Derived Cultures of Oilseed Rape , 1993, Plant physiology.

[23]  D. Taylor,et al.  Abscisic acid induction of elongase activity, biosynthesis and accumulation of very long chain monounsaturated fatty acids and oil body proteins in microspore-derived embryos of Brassica napus L. cv Reston☆ , 1992 .

[24]  R. Wing,et al.  Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements. , 1991, Genes & development.

[25]  D. Hardie,et al.  A new assay procedure to study the induction of β-ketoacyl-ACP synthase I and II, and the complete purification of β-ketoacyl-ACP synthase I from developing seeds of oilseed rape (Brassica napus) , 1989 .

[26]  C. Domoney,et al.  Measurement of gene number for seed storage proteins in Pisum. , 1985, Nucleic acids research.

[27]  D. Murphy,et al.  Biosynthesis of Seed Storage Products during Embryogenesis in Rapeseed, Brassica napus , 1989 .

[28]  W. Roesler,et al.  Cyclic AMP and the induction of eukaryotic gene transcription. , 1988, The Journal of biological chemistry.

[29]  T A McKeon,et al.  Purification and characterization of the stearoyl-acyl carrier protein desaturase and the acyl-acyl carrier protein thioesterase from maturing seeds of safflower. , 1982, The Journal of biological chemistry.