A discussion of enzyme reference materials: applications and specifications.

Clinical laboratories estimating enzyme activity in serum are using commercial lyophilized sera for four major purposes. These uses—as a standard and for intermethod, intramethod, and precision control—are segregated, and specifications for each deployment are examined in terms of requirements for the enzyme material: freedom from interfering or indicator enzymes and chromogens; high specific activity; inclusion of optimal cofactor concentrations; commutability, human properties and source; the presence of a single isoenzyme; and stability. The effects of serum matrix and variable assay conditions on the utility of enzyme materials are analyzed. Specifications differ for each enzyme material application. The compatibility of commercial lyophilized sera containing aspartate aminotransferase activity with several cited specifications is assessed

[1]  R. Rej,et al.  An L-aspartate: 2-oxoglutarate aminotransferase reference material from human erythrocytes: preparation and characterization. , 1972, Clinical Chemistry.

[2]  C. Salvatore,et al.  Serum glutamic oxalacetic transaminase activity: revised manual and automated methods using diazonium dyes. , 1971, American journal of clinical pathology.

[3]  J. Kelly,et al.  Clinical laboratory performance. Experience with New York State clinical laboratory program. , 1969, New York state journal of medicine.

[4]  A. Babson.  Standardization of Enzyme Analyses , 1969 .

[5]  R. Mccomb,et al.  Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity. , 1968, Clinical chemistry.

[6]  M. Kelley,et al.  Precision estimates in clinical chemistry. I. Variability of analytic results in a survey reference sample related to the use of a nonhuman serum alkaline phosphatase. , 1967, Clinical chemistry.

[7]  P. Williams,et al.  The use of a diazonium salt for the determination of glutamic-oxalacetic transaminase in serum. , 1962, Clinica chimica acta; international journal of clinical chemistry.

[8]  R. J. Henry,et al.  Revised spectrophotometric methods for the determination of glutamic-oxalacetic transaminase, glutamic-pyruvic transaminase, and lactic acid dehydrogenase. , 1960, Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists.

[9]  S REITMAN,et al.  A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. , 1957, American journal of clinical pathology.

[10]  G. Bucolo.  Testing commercial enzyme reference sera. , 1971, American journal of clinical pathology.

[11]  E. Amador,et al.  The accuracy of commercial enzyme reference sera. , 1970, American journal of clinical pathology.

[12]  A. Hamfelt.  The effect of pyridoxal phosphate on the aminotransferase assay in blood. , 1966, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[13]  A. Karmen.  A note on the spectrometric assay of glutamic-oxalacetic transaminase in human blood serum. , 1955, The Journal of clinical investigation.