Tetraspanin CD151 regulates α6β1 integrin adhesion strengthening

The tetraspanin CD151 molecule associates specifically with laminin-binding integrins, including α6β1. To probe strength of α6β1-dependent adhesion to laminin-1, defined forces (0–1.5 nN) were applied to magnetic laminin-coated microbeads bound to NIH 3T3 cells. For NIH 3T3 cells bearing wild-type CD151, adhesion strengthening was observed, as bead detachment became more difficult over time. In contrast, mutant CD151 (with the C-terminal region replaced) showed impaired adhesion strengthening. Static cell adhesion to laminin-1, and detachment of beads coated with fibronectin or anti-α6 antibody were all unaffected by CD151 mutation. Hence, CD151 plays a key role in selectively strengthening α6β1 integrin-mediated adhesion to laminin-1.

[1]  M. Hemler,et al.  An extracellular site on tetraspanin CD151 determines α3 and α6 integrin–dependent cellular morphology , 2002, The Journal of cell biology.

[2]  A. Bausch,et al.  Dynamic force spectroscopy to probe adhesion strength of living cells. , 2002, Physical review letters.

[3]  J. Weisel,et al.  Binding strength and activation state of single fibrinogen-integrin pairs on living cells , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[4]  A. Sonnenberg,et al.  Association of the tetraspanin CD151 with the laminin-binding integrinsα 3β1, α6β1, α6β4 and α7β1 in cells in culture and in vivo , 2002 .

[5]  M. Hemler,et al.  Specific tetraspanin functions , 2001, The Journal of cell biology.

[6]  F. Berditchevski.  Complexes of tetraspanins with integrins: more than meets the eye. , 2001, Journal of cell science.

[7]  S. Fitter,et al.  Analysis of the CD151·α3β1 Integrin and CD151·Tetraspanin Interactions by Mutagenesis* , 2001, The Journal of Biological Chemistry.

[8]  R D Kamm,et al.  Receptor-Based Differences in Human Aortic Smooth Muscle Cell Membrane Stiffness , 2001, Hypertension.

[9]  L. Gibson,et al.  Fibroblast contraction of a collagen-GAG matrix. , 2001, Biomaterials.

[10]  E. Zamir,et al.  Molecular complexity and dynamics of cell-matrix adhesions. , 2001, Journal of cell science.

[11]  R. Marchant,et al.  Force measurements on the molecular interactions between ligand (RGD) and human platelet αIIbβ3 receptor system , 2001 .

[12]  K. Sekiguchi,et al.  Laminin-10/11 and Fibronectin Differentially Regulate Integrin- dependent Rho and Rac Activation via p130Cas-CrkII-DOCK180 Pathway* , 2001, The Journal of Biological Chemistry.

[13]  M Essler,et al.  Rapid stiffening of integrin receptor-actin linkages in endothelial cells stimulated with thrombin: a magnetic bead microrheology study. , 2001, Biophysical journal.

[14]  M. Yáñez-Mó,et al.  Tetraspanins and Intercellular Interactions , 2001, Microcirculation.

[15]  V. Koteliansky,et al.  Global expression analysis of extracellular matrix-integrin interactions in monocytes. , 2000, Immunity.

[16]  D E Ingber,et al.  Analysis of cell mechanics in single vinculin-deficient cells using a magnetic tweezer. , 2000, Biochemical and biophysical research communications.

[17]  Ben Fabry,et al.  Mechanical control of cyclic AMP signalling and gene transcription through integrins , 2000, Nature Cell Biology.

[18]  M. Hemler,et al.  Transmembrane-4-superfamily proteins CD151 and CD81 associate with alpha 3 beta 1 integrin, and selectively contribute to alpha 3 beta 1-dependent neurite outgrowth. , 2000, Journal of cell science.

[19]  A. Sonnenberg,et al.  The Tetraspan Molecule Cd151, a Novel Constituent of Hemidesmosomes, Associates with the Integrin α6β4 and May Regulate the Spatial Organization of Hemidesmosomes , 2000, The Journal of cell biology.

[20]  Richard T. Lee,et al.  Direct Extracellular Contact between Integrin α3β1 and TM4SF Protein CD151* , 2000, The Journal of Biological Chemistry.

[21]  S. Abramson,et al.  Functional Genomic Analysis in Arthritis-Affected Cartilage: Yin-Yang Regulation of Inflammatory Mediators by α5β1 and αVβ3 Integrins1 , 2000, The Journal of Immunology.

[22]  Michael P. Sheetz,et al.  Keratocytes Pull with Similar Forces on Their Dorsal and Ventral Surfaces , 1999, The Journal of cell biology.

[23]  R M Hochmuth,et al.  Mechanical anchoring strength of L-selectin, beta2 integrins, and CD45 to neutrophil cytoskeleton and membrane. , 1999, Biophysical journal.

[24]  M A Horton,et al.  Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy. , 1999, Biochemical and biophysical research communications.

[25]  Robert E. Buxbaum,et al.  Direct Observations of the Mechanical Behaviors of the Cytoskeleton in Living Fibroblasts , 1999, The Journal of cell biology.

[26]  S. Fitter,et al.  PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function. , 1999, Journal of cell science.

[27]  M. Hemler,et al.  Interaction of the Integrin β1 Cytoplasmic Domain with ICAP-1 Protein* , 1999, The Journal of Biological Chemistry.

[28]  R. Yauch,et al.  Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration. , 1998, Molecular biology of the cell.

[29]  K. Jacobson,et al.  Local measurements of viscoelastic parameters of adherent cell surfaces by magnetic bead microrheometry. , 1998, Biophysical journal.

[30]  M. Hemler.  Integrin associated proteins. , 1998, Current opinion in cell biology.

[31]  L. Ashman,et al.  Regulation of Endothelial Cell Motility by Complexes of Tetraspan Molecules CD81/TAPA-1 and CD151/PETA-3 with α3β1 Integrin Localized at Endothelial Lateral Junctions , 1998, The Journal of cell biology.

[32]  Andrés J. García,et al.  Force Required to Break α5β1Integrin-Fibronectin Bonds in Intact Adherent Cells Is Sensitive to Integrin Activation State* , 1998, Journal of Biological Chemistry.

[33]  B. Nebe,et al.  ANALYSIS OF SPATIAL DISTRIBUTIONS OF CELLULAR MOLECULES DURING MECHANICAL STRESSING OF CELL SURFACE RECEPTORS USING CONFOCAL MICROSCOPY , 1998, Cell biology international.

[34]  Daniel Choquet,et al.  Extracellular Matrix Rigidity Causes Strengthening of Integrin–Cytoskeleton Linkages , 1997, Cell.

[35]  K. Yamada,et al.  Integrin function: molecular hierarchies of cytoskeletal and signaling molecules , 1995, The Journal of cell biology.

[36]  G. Bazzoni,et al.  Monoclonal Antibody 9EG7 Defines a Novel β1 Integrin Epitope Induced by Soluble Ligand and Manganese, but Inhibited by Calcium (*) , 1995, The Journal of Biological Chemistry.

[37]  E. Sage,et al.  Between molecules and morphology. Extracellular matrix and creation of vascular form. , 1995, The American journal of pathology.

[38]  D. Ingber,et al.  Probing transmembrane mechanical coupling and cytomechanics using magnetic twisting cytometry. , 1995, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[39]  D. Ingber,et al.  Mechanotransduction across the cell surface and through the cytoskeleton , 1993 .

[40]  D. Ingber,et al.  Integrins as mechanochemical transducers. , 1991, Current opinion in cell biology.

[41]  D. McClay,et al.  Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response , 1989, The Journal of cell biology.

[42]  Richard O. Hynes,et al.  Integrins: A family of cell surface receptors , 1987, Cell.

[43]  M. Hemler,et al.  Function of the Tetraspanin CD151–α6β1 Integrin Complex during Cellular Morphogenesis , 2002 .

[44]  E. Sackmann,et al.  Measurement of local viscoelasticity and forces in living cells by magnetic tweezers. , 1999, Biophysical journal.

[45]  K. Burridge,et al.  Focal adhesions, contractility, and signaling. , 1996, Annual review of cell and developmental biology.

[46]  Kenneth M. Yamada,et al.  Integrin transmembrane signaling and cytoskeletal control. , 1995, Current opinion in cell biology.

[47]  G. Davis,et al.  Regulation of endothelial cell morphogenesis by integrins, mechanical forces, and matrix guidance pathways. , 1995, Experimental cell research.