Expression and processing of the activin-A/erythroid differentiation factor precursor: a member of the transforming growth factor-beta superfamily.

The biosynthesis and intracellular processing of the polypeptide precursor of the beta A-chain of the fertility hormone inhibin were assessed by infecting a wide spectrum of cell types with a recombinant vaccinia virus. Most cell lines, including follicular granulosa cells, secrete both prohormone and mature hormone as homodimers (activin) composed of disulfide-linked subunits of 54 kDa (proactivin-A) and 14 kDa (activin-A), respectively, and a small amount of prohormone-mature hormone heterodimers. Mature activin is secreted from mouse pituitary cells (AtT-20), while pig kidney cells [PK(15)] secrete mostly proactivin. More prohormone is secreted in the presence of NH4Cl, suggesting that prohormone processing is facilitated by low pH. Proactivin-A is not a ligand for the mannose-6-phosphate/insulin growth factor-II receptor. The recombinant activin stimulates FSH release from pituitary cells and differentiates erythroleukemia cell lines in vitro.

[1]  A. Mason,et al.  Activin B: precursor sequences, genomic structure and in vitro activities. , 1989, Molecular endocrinology.

[2]  S. Kornfeld,et al.  Mannose 6-phosphate receptors and lysosomal enzyme targeting. , 1989, The Journal of biological chemistry.

[3]  A. Brunner,et al.  Transforming growth factor beta 1: importance of glycosylation and acidic proteases for processing and secretion. , 1989, Molecular endocrinology.

[4]  H. Shibai,et al.  Identification of the two types of specific receptor for activin/EDF expressed on Friend leukemia and embryonal carcinoma cells. , 1989, Biochemical and biophysical research communications.

[5]  E. Chen,et al.  Vgr-1, a mammalian gene related to Xenopus Vg-1, is a member of the transforming growth factor beta gene superfamily. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[6]  L. Madisen,et al.  Expression and Characterization of Recombinant TGF-β2 Proteins Produced in Mammalian Cells , 1989 .

[7]  V. Rosen,et al.  Novel regulators of bone formation: molecular clones and activities. , 1988, Science.

[8]  A. Mason,et al.  Selective and indirect modulation of human multipotential and erythroid hematopoietic progenitor cell proliferation by recombinant human activin and inhibin. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[9]  R. Derynck,et al.  A new type of transforming growth factor‐beta, TGF‐beta 3. , 1988, The EMBO journal.

[10]  N. Ling,et al.  A surface component on GH3 pituitary cells that recognizes transforming growth factor-beta, activin, and inhibin. , 1988, The Journal of biological chemistry.

[11]  K. Titani,et al.  Identification of a specific receptor for erythroid differentiation factor on follicular granulosa cell. , 1988, The Journal of biological chemistry.

[12]  A. Brunner,et al.  Identification of mannose 6-phosphate in two asparagine-linked sugar chains of recombinant transforming growth factor-beta 1 precursor. , 1988, The Journal of biological chemistry.

[13]  J. Vaughan,et al.  Inhibin β in central neural pathways involved in the control of oxytocin secretion , 1988, Nature.

[14]  P. ten Dijke,et al.  Identification of another member of the transforming growth factor type beta gene family. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[15]  W. Rutter,et al.  Processing and secretion of nerve growth factor: expression in mammalian cells with a vaccinia virus vector , 1988, Molecular and cellular biology.

[16]  S. Ying.  Inhibins, activins, and follistatins: gonadal proteins modulating the secretion of follicle-stimulating hormone. , 1988, Endocrine reviews.

[17]  Jonathan A. Cooper,et al.  Recombinant type 1 transforming growth factor beta precursor produced in Chinese hamster ovary cells is glycosylated and phosphorylated , 1988, Molecular and cellular biology.

[18]  H. Shibai,et al.  Erythroid differentiation factor is encoded by the same mRNA as that of the inhibin beta A chain. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[19]  H. Stunnenberg,et al.  High expression of functional adenovirus DNA polymerase and precursor terminal protein using recombinant vaccinia virus. , 1988, Nucleic acids research.

[20]  I. Mellman,et al.  The mannose 6-phosphate receptor and the biogenesis of lysosomes , 1988, Cell.

[21]  D. L. Weeks,et al.  A maternal mRNA localized to the vegetal hemisphere in xenopus eggs codes for a growth factor related to TGF-β , 1987, Cell.

[22]  B. Haendler,et al.  Complementary DNA for human glioblastoma‐derived T cell suppressor factor, a novel member of the transforming growth factor‐beta gene family. , 1987, The EMBO journal.

[23]  A. Brunner,et al.  Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells , 1987, Molecular and cellular biology.

[24]  M. Sporn,et al.  Some recent advances in the chemistry and biology of transforming growth factor-beta , 1987, The Journal of cell biology.

[25]  Teresa K. Woodruff,et al.  Rat Inhibin: Molecular Cloning of α- and β-Subunit Complementary Deoxyribonucleic Acids and Expression in the Ovary , 1987 .

[26]  J. Cazenave,et al.  Choosing a Host Cell for Active Recombinant Factor VIII Production Using Vaccinia Virus , 1987, Bio/Technology.

[27]  J. Graycar,et al.  Cartilage-inducing factor-B is a unique protein structurally and functionally related to transforming growth factor-beta. , 1987, The Journal of biological chemistry.

[28]  D. Mead,et al.  Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. , 1986, Protein engineering.

[29]  R. Evans,et al.  Inhibin A-subunit cDNAs from porcine ovary and human placenta. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[30]  M. Zerial,et al.  The transmembrane segment of the human transferrin receptor functions as a signal peptide. , 1986, The EMBO journal.

[31]  D. Hruby,et al.  Expression and cell type--specific processing of human preproenkephalin with a vaccinia recombinant. , 1986, Science.

[32]  Joan Vaughan,et al.  Purification and characterization of an FSH releasing protein from porcine ovarian follicular fluid , 1986, Nature.

[33]  Naoto Ueno,et al.  Pituitary FSH is released by a heterodimer of the β-subunits from the two forms of inhibin , 1986, Nature.

[34]  P. Donahoe,et al.  Isolation of the bovine and human genes for müllerian inhibiting substance and expression of the human gene in animal cells , 1986, Cell.

[35]  M T Hearn,et al.  Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[36]  P. Seeburg,et al.  Structure of two human ovarian inhibins. , 1986, Biochemical and biophysical research communications.

[37]  N. Ling,et al.  Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor-β , 1985, Nature.

[38]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[39]  A. Brunner,et al.  Site-directed Mutagenesis of Cysteine Residues in the Pro Region of the Transforming Growth Factor 81 Precursor EXPRESSION AND CHARACTERIZATION MUTANT PROTEINS* , 2001 .

[40]  I. Mellman,et al.  The biogenesis of lysosomes. , 1989, Annual review of cell biology.

[41]  W. Vale,et al.  Gonadal and extragonadal expression of inhibin alpha, beta A, and beta B subunits in various tissues predicts diverse functions. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[42]  W. Gelbart,et al.  A transcript from a Drosophila pattern gene predicts a protein homologous to the transforming growth factor-β family , 1987, Nature.

[43]  I Mellman,et al.  Acidification of the endocytic and exocytic pathways. , 1986, Annual review of biochemistry.

[44]  F. Takaku,et al.  Long-term cultivation and differentiation of human erythroleukemia cells in a protein-free chemically defined medium. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[45]  P. Franchimont,et al.  Identification in human seminal fluid of an inhibin-like factor which selectively regulates FSH secretion. , 1979, Journal of reproduction and fertility. Supplement.