Stimulation of Vascular Smooth Muscle Sodium, Potassium—Adenosinetriphosphatase by Vasodilators

A ouabain-sensitive, Mg2+-dependent, Na+, K+-stimulated adenosinetriphosphatase (ATPase) isolated from canine mesenteric arteries was activated by the following vasodilators: hydralazine, diazoxide, PGE1, PGE2, PGA2, and minoxidil. Epinephrine, norepinephrine, and isoproterenol also stimulated the ATPase, but PGF2α was ineffective. Since these vasodilators activate the adenylate cyclase of vascular smooth muscle, the effects of cyclic adenosine monophosphate (AMP) and theophylline on the Na+, K+-ATPase were studied; both substances caused a concentration-dependent increase in enzymatic activity. Propranolol blocked the catecholamine-induced stimulation of Na+, K+-ATPase, and polyphloretin phosphate antagonized the effects of the prostaglandins. It is concluded that vasodilatation in response to these substances is associated with the stimulation of the Na+, K+-ATPase of vascular smooth muscle probably mediated through cyclic AMP.

[1]  O. H. Lowry,et al.  The determination of inorganic phosphate in the presence of labile phosphate esters. , 1946, The Journal of biological chemistry.

[2]  L. Hurwitz,et al.  The link between agonist action and response in smooth muscle. , 1971, Annual review of pharmacology.

[3]  E. Landon,et al.  A Calcium Pump in Vascular Smooth Muscle , 1972, Science.

[4]  F. Sunahara,et al.  Inhibition of prostaglandin effects by ouabain in the canine vascular tissue. , 1969, Canadian journal of physiology and pharmacology.

[5]  A. Somlyo,et al.  Vascular smooth muscle. II. Pharmacology of normal and hypotensive vessels. , 1970, Pharmacological reviews.

[6]  A. Gilman.  A protein binding assay for adenosine 3':5'-cyclic monophosphate. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[7]  A. Hodgkin,et al.  The influence of calcium on sodium efflux in squid axons , 1969, The Journal of physiology.

[8]  G. Nahas,et al.  CYCLIC AMP AND SMOOTH MUSCLE FUNCTION * , 1971, Annals of the New York Academy of Sciences.

[9]  Somlyo Ap.  Excitation-contraction coupling in vertebrate smooth muscle: correlation of ultrastructure with function. , 1972 .

[10]  R. Andersson,et al.  Cyclic AMP and calcium in relaxation in intestinal smooth muscle. , 1972, Nature: New biology.

[11]  J. Lowenstein,et al.  Preparation and properties of 5'-nucleotidase from smooth muscle of small intestine. , 1970, The Journal of biological chemistry.

[12]  J. Charnock,et al.  The effect of potassium and sodium ions on rat-liver mitochondrial oxidative phosphorylation. , 1965, Biochimica et biophysica acta.

[13]  H. Reuter,et al.  The effect of the internal sodium concentration on calcium fluxes in isolated guinea‐pig auricles , 1970, The Journal of physiology.

[14]  D. Anderson,et al.  The Mechanism of the Vasodilator Action of Potassium 1 , 1972, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[15]  S. Fermandjian,et al.  Calcium Release induced by Interaction of Angiotensin with its Receptors in Smooth Muscle Cell Microsomes , 1972, Nature.

[16]  R. Andersson.  Role of cyclic AMP and Ca ++ in mechanical and metabolic events in isometrically contracting vascular smooth muscle. , 1973, Acta physiologica Scandinavica.

[17]  G. Sachs,et al.  Acetyl phosphatase in brain microsomes: A partial reaction of Na+ + K+ ATPase☆ , 1967 .

[18]  A. Somlyo,et al.  R SMOOTH MUSCLE , 1970 .

[19]  H. Matsui,et al.  Purification and properties of a highly active ouabain-sensitive Na+, K+-dependent adenosinetriphosphatase from cardiac tissue. , 1966, Biochimica et biophysica acta.

[20]  A. Gornall,et al.  Determination of serum proteins by means of the biuret reaction. , 1949, The Journal of biological chemistry.

[21]  A. Somlyo,et al.  Electromechanical and pharmacomechanical coupling in vascular smooth muscle. , 1968, The Journal of pharmacology and experimental therapeutics.

[22]  L. Volicer,et al.  Effect of catecholamines and angiotensin on cyclic AMP in rat aorta and tail artery. , 1971, European journal of pharmacology.

[23]  D. Anderson,et al.  The mechanism of the vasodilator action of potassium. , 1971, The Journal of laboratory and clinical medicine.

[24]  G. Langer,et al.  Effects of strophanthidin upon contraction and ionic exchange in rabbit ventricular myocardium: relation to control of active state. , 1970, Journal of molecular and cellular cardiology.

[25]  J. Miller,et al.  Antagonism of some smooth muscle actions of prostaglandins by polyphloretin phosphate , 1970, British journal of pharmacology.

[26]  D. Bohr.  ELECTROLYTES AND SMOOTH MUSCLE CONTRACTION. , 1964, Pharmacological reviews.

[27]  E. Daniel,et al.  Sodium-potassium stimulated adenosinetriphosphatase of vascular smooth muscle. , 1971, Canadian journal of biochemistry.

[28]  J. Sobieski,et al.  Possible role of sodium-calcium pumps in tension development of vascular smooth muscle. , 1969, Microvascular research.