Ascorbic Acid Oxidase Activity in Apple Buds: Relation to Thidiazuron- induced Lateral Budbreak

The activity of ascorbic acid oxidase (AAO) was studied in apple (Malus domestica Borkh.) buds during dormancy and thidiazuron-induced budbreak. In dor- mant buds, activity of AAO was low compared with buds that were treated with thidiazuron and had resumed growth. An increase in AAO activity began at the time of metabolic transition from dormancy to budbreak. The highest level of activity was reached 10 days after thidiazuron induction during the expansion growth phase. In vitro AAO activity of apple bud extract was increased by addition of Cu (CuSO 4) and inhibited by Cu-chelating agents, diethyldithiocarbamate (DDC), sodium azide (NaN 3), and 8-hydroxyquinoiine (8-OH-Q). In vivo treatment of apple buds with Cu-chelating agents inhibited AAO activity and bud growth but not budbreak. Chemical name used: N- phenyl -N' -1,2,3-thidiazol-5-ylurea (thidiazuron).

[1]  G. Lang,et al.  Self- and Cross-pollination Affect Stigmatic Pollen Saturation in Blueberry , 1992 .

[2]  S. Y. Wang,et al.  Changes in superoxide dismutase activity during thidiazuron-induced lateral budbreak of apple , 1991 .

[3]  M. Faust,et al.  Changes in ascorbate, glutathione, and related enzyme activities during thidiazuron‐induced bud break of apple , 1991 .

[4]  J. Varner,et al.  Expression of Ascorbic Acid Oxidase in Zucchini Squash (Cucurbita pepo L.). , 1991, Plant physiology.

[5]  Miklos Faust,et al.  Physiology of Temperate Zone Fruit Trees , 1989 .

[6]  A. Shinmyō,et al.  Primary structure of cucumber (Cucumis sativus) ascorbate oxidase deduced from cDNA sequence: homology with blue copper proteins and tissue-specific expression. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[7]  M. Esaka,et al.  Secretion of ascorbate oxidase by suspension-cultured Pumpkin cells , 1989 .

[8]  M. Esaka,et al.  Formation of ascorbate oxidase in cultured pumpkin cells , 1988 .

[9]  F. L. Crane,et al.  The Role of Ascorbate in Biomembrane Energetics a , 1987, Annals of the New York Academy of Sciences.

[10]  H. Evans,et al.  Enzymatic reactions of ascorbate and glutathione that prevent peroxide damage in soybean root nodules. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[11]  M. Faust,et al.  Breaking bud dormancy in apple with a plant bioregulator, thidiazuron. , 1986 .

[12]  S. Sagisaka,et al.  Increase in Enzyme Activities Related to Ascorbate Metabolism during Cold Acclimation in Poplar Twigs , 1984 .

[13]  B. Halliwell.  Ascorbic Acid and the Illuminated Chloroplast , 1982 .

[14]  T. Kurata,et al.  A rapid and sensitive method for the determination of ascorbic acid using 4,7-diphenyl-1,10-phenanthroline , 1981 .

[15]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[16]  D. Mertz.  DISTRIBUTION AND CELLULAR LOCALIZATION OF ASCORBIC ACID OXIDASE IN THE MAIZE ROOT TIP , 1961 .

[17]  W. Jensen,et al.  THE CYTOCHEMICAL LOCALIZATION OF ASCORBIC ACID IN ROOT TIP CELLS , 1956, The Journal of Biophysical and Biochemical Cytology.

[18]  S. I. Honda.  Ascorbic Acid Oxidase in Barley Roots. , 1955, Plant physiology.

[19]  E. Newcomb,et al.  Effect of Auxin on Ascorbic Oxidase Activity in Tobacco Pith Cells.∗ , 1951, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[20]  E. Waygood.  PHYSIOLOGICAL AND BIOCHEMICAL STUDIES IN PLANT METABOLISM: II. RESPIRATORY ENZYMES IN WHEAT , 1950 .

[21]  M. E. Reid.  RELATION OF VITAMIN C TO CELL SIZE IN THE GROWING REGION OF THE PRIMARY ROOT OF COWPEA SEEDLINGS , 1941 .