Protein interactions with the glucose transporter binding protein GLUT1CBP that provide a link between GLUT1 and the cytoskeleton.

Subcellular targeting and the activity of facilitative glucose transporters are likely to be regulated by interactions with cellular proteins. This report describes the identification and characterization of a protein, GLUT1 C-terminal binding protein (GLUT1CBP), that binds via a PDZ domain to the C terminus of GLUT1. The interaction requires the C-terminal four amino acids of GLUT1 and is isoform specific because GLUT1CBP does not interact with the C terminus of GLUT3 or GLUT4. Most rat tissues examined contain both GLUT1CBP and GLUT1 mRNA, whereas only small intestine lacked detectable GLUT1CBP protein. GLUT1CBP is also expressed in primary cultures of neurons and astrocytes, as well as in Chinese hamster ovary, 3T3-L1, Madin-Darby canine kidney, Caco-2, and pheochromocytoma-12 cell lines. GLUT1CBP is able to bind to native GLUT1 extracted from cell membranes, self-associate, or interact with the cytoskeletal proteins myosin VI, alpha-actinin-1, and the kinesin superfamily protein KIF-1B. The presence of a PDZ domain places GLUT1CBP among a growing family of structural and regulatory proteins, many of which are localized to areas of membrane specialization. This and its ability to interact with GLUT1 and cytoskeletal proteins implicate GLUT1CBP in cellular mechanisms for targeting GLUT1 to specific subcellular sites either by tethering the transporter to cytoskeletal motor proteins or by anchoring the transporter to the actin cytoskeleton.

[1]  P. Chavrier,et al.  Molecular Biology of the Cell , 1990, Color Atlas of Clinical Hematology.

[2]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[3]  F. Ismail-Beigi,et al.  Activation of Glut1 glucose transporter in human erythrocytes. , 1998, Archives of biochemistry and biophysics.

[4]  A. Bretscher,et al.  An Apical PDZ Protein Anchors the Cystic Fibrosis Transmembrane Conductance Regulator to the Cytoskeleton* , 1998, The Journal of Biological Chemistry.

[5]  R. Lefkowitz,et al.  GTPase Activating Specificity of RGS12 and Binding Specificity of an Alternatively Spliced PDZ (PSD-95/Dlg/ZO-1) Domain* , 1998, The Journal of Biological Chemistry.

[6]  C. Montell TRP trapped in fly signaling web , 1998, Current Opinion in Neurobiology.

[7]  S. Shenolikar,et al.  The β2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange , 1998, Nature.

[8]  R. Rousset,et al.  The C-terminus of the HTLV-1 Tax oncoprotein mediates interaction with the PDZ domain of cellular proteins , 1998, Oncogene.

[9]  S. Almo,et al.  The modular structure of actin-regulatory proteins. , 1998, Current opinion in cell biology.

[10]  E. Snapp,et al.  Cytoskeletal Association Is Important for Differential Targeting of Glucose Transporter Isoforms in Leishmania , 1997, The Journal of cell biology.

[11]  W. Kuo,et al.  Actinin-associated LIM Protein: Identification of a Domain Interaction between PDZ and Spectrin-like Repeat Motifs , 1997, The Journal of cell biology.

[12]  K. Makino,et al.  Cloning and characterization of NE-dlg: a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein , 1997, Oncogene.

[13]  L. Cantley,et al.  Recognition of Unique Carboxyl-Terminal Motifs by Distinct PDZ Domains , 1997, Science.

[14]  S. Baldwin,et al.  Streptozotocin diabetes and the expression of GLUT1 at the brush border and basolateral membranes of intestinal enterocytes , 1996, FEBS letters.

[15]  J. Slot,et al.  Differential targeting of facilitative glucose transporters in polarized epithelial cells. , 1996, The American journal of physiology.

[16]  M. Fisher,et al.  Translocation of GLUT1 Does Not Account for Elevated Glucose Transport in Glucose-deprived 3T3-L1 Adipocytes (*) , 1996, The Journal of Biological Chemistry.

[17]  R. C. Bunn,et al.  C-terminal Mutations That Alter the Turnover Number for 3-O-Methylglucose Transport by GLUT1 and GLUT4 (*) , 1996, The Journal of Biological Chemistry.

[18]  B. Shieh,et al.  Regulation of the TRP Ca2+ Channel by INAD in Drosophila Photoreceptors , 1996, Neuron.

[19]  Stuart K. Kim,et al.  LET-23 Receptor Localization by the Cell Junction Protein LIN-7 during C. elegans Vulval Induction , 1996, Cell.

[20]  Y. Jan,et al.  Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases , 1995, Nature.

[21]  F. Maher Immunolocalization of GLUT1 and GLUT3 glucose transporters in primary cultured neurons and glia , 1995, Journal of neuroscience research.

[22]  M. Kasuga,et al.  Analysis of the structural features of the C-terminus of GLUT1 that are required for transport catalytic activity. , 1995, The Biochemical journal.

[23]  H. Kurachi,et al.  Expression and localization of glucose transporter 1 (GLUT1) in the rat oviduct: a possible supplier of glucose to embryo during early embryonic development. , 1995, Biochemical and biophysical research communications.

[24]  F. Ismail-Beigi,et al.  Modulation of GLUT1 Intrinsic Activity in Clone 9 Cells by Inhibition of Oxidative Phosphorylation (*) , 1995, The Journal of Biological Chemistry.

[25]  Y. Shi,et al.  ATP-sensitive Binding of a 70-kDa Cytosolic Protein to the Glucose Transporter in Rat Adipocytes (*) , 1995, The Journal of Biological Chemistry.

[26]  I. Macara,et al.  Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A , 1995, Molecular and cellular biology.

[27]  N. Hirokawa,et al.  KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria , 1994, Cell.

[28]  K. Simons,et al.  Involvement of microtubule motors in basolateral and apical transport in kidney cells , 1994, Nature.

[29]  S. Kain,et al.  Human multiple tissue western blots: a new immunological tool for the analysis of tissue-specific protein expression. , 1994, BioTechniques.

[30]  T. Hasson,et al.  Porcine myosin-VI: characterization of a new mammalian unconventional myosin , 1994, The Journal of cell biology.

[31]  D. Goodenough,et al.  Molecular characterization and tissue distribution of ZO-2, a tight junction protein homologous to ZO-1 and the Drosophila discs-large tumor suppressor protein , 1994, The Journal of cell biology.

[32]  M. Mueckler Facilitative glucose transporters. , 1994, European journal of biochemistry.

[33]  M. Palacín,et al.  High and polarized expression of GLUT1 glucose transporters in epithelial cells from mammary gland: acute down-regulation of GLUT1 carriers by weaning. , 1994, Endocrinology.

[34]  W. Sivitz,et al.  Immunolocalization of GLUT-1 glucose transporter in rat skeletal muscle and in normal and hypoxic cardiac tissue. , 1993, The American journal of physiology.

[35]  F. Ismail-Beigi,et al.  Rapid activation of GLUT-1 glucose transporter following inhibition of oxidative phosphorylation in clone 9 cells. , 1993, The Journal of biological chemistry.

[36]  C. Jung,et al.  Interaction of facilitative glucose transporter with glucokinase and its modulation by ADP and glucose‐6‐phosphate , 1993, Journal of cellular physiology.

[37]  J. Uitto,et al.  Glucose Transporters of Rat Peripheral Nerve: Differential Expression of GLUT1 Gene by Schwann Cells and Perineurial Cells In Vivo and In Vitro , 1992, Diabetes.

[38]  E. Van Obberghen,et al.  Potential involvement of the carboxy-terminus of the Glut 1 transporter in glucose transport. , 1992, Endocrinology.

[39]  Y. Jan,et al.  Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel. , 1992, Science.

[40]  D. S. Harris,et al.  Polarized distribution of glucose transporter isoforms in Caco-2 cells. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[41]  K. Mostov,et al.  Plasma membrane protein sorting in polarized epithelial cells , 1992, The Journal of cell biology.

[42]  S. Harik,et al.  Defective glucose transport across the blood-brain barrier as a cause of persistent hypoglycorrhachia, seizures, and developmental delay. , 1991, The New England journal of medicine.

[43]  M. Czech,et al.  Protein synthesis inhibitors activate glucose transport without increasing plasma membrane glucose transporters in 3T3-L1 adipocytes. , 1991, The Journal of biological chemistry.

[44]  W. Pardridge,et al.  Brain-type glucose transporter (GLUT-1) is selectively localized to the blood-brain barrier. Studies with quantitative western blotting and in situ hybridization. , 1990, The Journal of biological chemistry.

[45]  C. Jung,et al.  An ATP-modulated specific association of glyceraldehyde-3-phosphate dehydrogenase with human erythrocyte glucose transporter. , 1990, The Journal of biological chemistry.

[46]  T. Asano,et al.  C-terminal truncated glucose transporter is locked into an inward-facing form without transport activity , 1990, Nature.

[47]  M. X. Zuber,et al.  Elevation of the number of cell-surface insulin receptors and the rate of 2-deoxyglucose uptake by exposure of 3T3-L1 adipocytes to tolbutamide. , 1985, The Journal of biological chemistry.

[48]  M. Saraste,et al.  FEBS Lett , 2000 .

[49]  S. K. Kim,et al.  The C. elegans vulval induction gene lin-2 encodes a member of the MAGUK family of cell junction proteins. , 1996, Development.

[50]  J. Stow,et al.  Protein trafficking and polarity in kidney epithelium: from cell biology to physiology. , 1996, Physiological reviews.

[51]  F. Brosius,et al.  Immunogold localization of high-affinity glucose transporter isoforms in normal rat kidney. , 1995, Laboratory investigation; a journal of technical methods and pathology.

[52]  H. Sambrook Molecular cloning : a laboratory manual. Cold Spring Harbor, NY , 1989 .