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The analytical determination of pharmaceutical, food and environmental samples are a necessary strategy for study of quality of biological samples, food product, ground and wastewaters samples. Various analytical methods, such as chromatography and spectroscopy, have long been used for this purpose. With the advancement of technology and the growth of electrochemical methods, today the analytical techniques based on the use of electrochemical methods have grown significantly. On the other hand, the possibility of modifying electrochemical sensors to increase selectivity in food, pharmaceutical or environmental compounds analysis has led to the attention of many scientists to these techniques. This special issue focused for introduction new electrochemical modified sensors as analytical approach in determination of biological, pharmaceutical, environmental and food samples.