Detecting Interactions between the Renal Autoregulation Mechanisms in Time and Space

<italic>Objective:</italic> Our objective is to identify localized interactions between the renal autoregulation mechanisms over time. <italic>Methods:</italic> A time-varying phase-randomized wavelet bicoherence detector for quadratic phase coupling between tubuloglomerular feedback and the myogenic response is presented. Through simulations we show its ability to interrogate quadratic phase coupling. The method is applied to kidney blood flow and laser speckle imaging sequences of cortical perfusion from anesthetized rats before and after nonselective inhibition of nitric-oxide synthase. <italic>Results:</italic> Quadratic phase coupling in kidney blood flow data was present in four out of nine animals during the control period for 13.0 ± 5.6% (mean ± SD) of time and in five out of nine animals during inhibition of nitric-oxide synthase for 15.8 ± 8.2% of time. Approximately 60% of time-series extracted from laser speckle imaging pixels of the renal cortex showed significant quadratic phase coupling. Pixels with significant coupling had a median coupling length of 10.8 ± 2.2% and 12.1 ± 3.1% of time with the 95th percentile of pixels being coupled for 25.5 ± 4.4% and 30.9 ± 6.4% of time during control and inhibition of nitric-oxide synthase, respectively. <italic>Conclusion:</italic> These results indicate quadratic phase coupling exists in short time intervals between tubuloglomerular feedback and the myogenic response and is detected more often in local renal perfusion signals than whole kidney blood flow in anesthetized rats. <italic>Significance:</italic> Combining the detector and laser speckle imaging provides identification of coordination between renal autoregulation mechanisms that is localized in time and space.