Improved lycopene extraction from tomato peels using cell-wall degrading enzymes

Four commercial enzyme preparations with pectinolytic, cellulolytic and hemicellulolytic activities were tested for their ability to enhance lycopene extraction from tomato peels. Screening experiments were performed at 25 °C by subjecting the peels to a 4-h enzyme incubation followed by 1-h hexane extraction. Peclyve EP and LI were the most efficient, with an almost 20-fold increase in extraction yield. Peclyve LI was used to evaluate the influence of solvent type and enzyme incubation time on lycopene recovery. Hexane, ethyl acetate and the mixture hexane/acetone/ethanol 50:25:25 (v/v) were used as solvents. Under the best extraction conditions (1-h enzyme incubation followed by a 3-h solvent extraction at 40 °C) up to 440 mg of lycopene per 100 g of dry tomato peels were obtained. The percentage recoveries were in the range of 3–30%, for the untreated peels, and 77–98% for the enzymatically treated material.

[1]  A. Spurr,et al.  CHROMOPLASTS OF TOMATO FRUITS. II. THE RED TOMATO , 1969 .

[2]  K. Gross.  Fractionation and partial characterization of cell walls from normal and non‐ripening mutant tomato fruit , 1984 .

[3]  P. Di Mascio,et al.  Lycopene as the most efficient biological carotenoid singlet oxygen quencher. , 1989, Archives of biochemistry and biophysics.

[4]  M. Balaban,et al.  Optimization of Carrot Juice Color and Cloud Stability , 1993 .

[5]  J. Lema,et al.  Enzymatic pretreatment to enhance oil extraction from fruits and oilseeds: a review , 1994 .

[6]  Roger M. Rowell,et al.  Swelling of compressed cellulose fiber webs in organic liquids , 1995, Cellulose.

[7]  M. Maguer,et al.  Lycopene in tomatoes and tomato pulp fractions , 1996 .

[8]  Susan Budavari,et al.  The Merck index , 1998 .

[9]  F. M. Gama,et al.  Selective enzyme-mediated extraction of capsaicinoids and caratenoids from chili guajillo puya (Capsicum annum L.) using ethanol as solvent. , 2000, Journal of agricultural and food chemistry.

[10]  K. Wakabayashi.  Changes in Cell Wall Polysaccharides During Fruit Ripening , 2000, Journal of Plant Research.

[11]  Penelope Perkins-Veazie,et al.  A Quantitative Assay for Lycopene That Utilizes Reduced Volumes of Organic Solvents , 2002 .

[12]  John Shi,et al.  Lycopene from Tomatoes , 2002 .

[13]  E. Bárzana,et al.  Enzyme-mediated solvent extraction of carotenoids from marigold flower (Tagetes erecta). , 2002, Journal of agricultural and food chemistry.

[14]  B. Watkins,et al.  Supercritical fluid extraction of lycopene from tomato processing byproducts. , 2002, Journal of agricultural and food chemistry.

[15]  E. Sabio,et al.  Lycopene and β-Carotene Extraction from Tomato Processing Waste Using Supercritical CO2 , 2003 .

[16]  V. Böhm,et al.  Cleavage products of lycopene produced by in vitro oxidations: characterization and mechanisms of formation. , 2003, Journal of agricultural and food chemistry.

[17]  J. Gril,et al.  Swelling of acetylated wood I. Swelling in organic liquids , 2005, Journal of Wood Science.

[18]  J. López-Roca,et al.  Improving colour extraction and stability in red wines: the use of maceration enzymes and enological tannins , 2005 .

[19]  E. Baldwin,et al.  Hydrolysis of orange peel with pectinase and cellulase enzymes , 1992, Biotechnology Letters.

[20]  R. Aluko,et al.  The anti-carcinogenic and anti-atherogenic effects of lycopene: a review , 2005 .

[21]  E. Giovannucci.  Tomato products, lycopene, and prostate cancer: a review of the epidemiological literature. , 2005, The Journal of nutrition.

[22]  G. Mittal,et al.  Synergistic Anti-Oxidative Effects of Lycopene with Other Bioactive Compounds , 2005 .

[23]  Y. Kakùda,et al.  Stability of lycopene during food processing and storage. , 2005, Journal of medicinal food.

[24]  İnci Çinar,et al.  Effects of cellulase and pectinase concentrations on the colour yield of enzyme extracted plant carotenoids , 2005 .

[25]  A. Barba,et al.  Analytical, Nutritional and Clinical Methods , 2022 .

[26]  Laxmi Ananthanarayan,et al.  Enzyme aided extraction of lycopene from tomato tissues , 2007 .

[27]  I. Arvanitoyannis,et al.  Vegetable Waste Treatment: Comparison and Critical Presentation of Methodologies , 2008, Critical reviews in food science and nutrition.

[28]  Cristina M. Oliveira,et al.  Cell wall modifications during fruit ripening: when a fruit is not the fruit , 2008 .