A facile technique to prepare biodegradable coaxial electrospun nanofibers for controlled release of bioactive agents.

A one-step, mild procedure based on coaxial electrospinning was developed for incorporation and controlled release of two model proteins, BSA and lysozyme, from biodegradable core-shell nanofibers with PCL as shell and protein-containing PEG as core. The thickness of the core and shell could be adjusted by the feed rate of the inner dope, which in turn affected the release profiles of the incorporated proteins. It was revealed that the released lysozyme maintained its structure and bioactivity. The current method may find wide applications for controlled release of proteins and tissue engineering.

[1]  Wenlei Jiang,et al.  Stabilization and Controlled Release of Bovine Serum Albumin Encapsulated in Poly(D, L-lactide) and Poly(ethylene glycol) Microsphere Blends , 2001, Pharmaceutical Research.

[2]  K E Healy,et al.  A biodegradable polymer scaffold for delivery of osteotropic factors. , 2000, Biomaterials.

[3]  Younan Xia,et al.  Direct Fabrication of Composite and Ceramic Hollow Nanofibers by Electrospinning , 2004 .

[4]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[5]  Benjamin Chu,et al.  Structure and morphology changes during in vitro degradation of electrospun poly(glycolide-co-lactide) nanofiber membrane. , 2003, Biomacromolecules.

[6]  Darrell H. Reneker,et al.  Beaded nanofibers formed during electrospinning , 1999 .

[7]  J. Vacanti,et al.  Contractile cardiac grafts using a novel nanofibrous mesh. , 2004, Biomaterials.

[8]  A. Klibanov,et al.  FTIR characterization of the secondary structure of proteins encapsulated within PLGA microspheres. , 1999, Journal of controlled release : official journal of the Controlled Release Society.

[9]  R. Tuan,et al.  A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells. , 2005, Biomaterials.

[10]  Kwangsok Kim,et al.  Incorporation and controlled release of a hydrophilic antibiotic using poly(lactide-co-glycolide)-based electrospun nanofibrous scaffolds. , 2004, Journal of controlled release : official journal of the Controlled Release Society.

[11]  Seeram Ramakrishna,et al.  Preparation of Core−Shell Structured PCL-r-Gelatin Bi-Component Nanofibers by Coaxial Electrospinning , 2004 .

[12]  Lixin Yang,et al.  Biodegradable electrospun fibers for drug delivery. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[13]  Cato T Laurencin,et al.  Bioresorbable nanofiber-based systems for wound healing and drug delivery: optimization of fabrication parameters. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[14]  John Layman,et al.  Release of tetracycline hydrochloride from electrospun poly(ethylene-co-vinylacetate), poly(lactic acid), and a blend. , 2002, Journal of controlled release : official journal of the Controlled Release Society.

[15]  W Wang,et al.  Instability, stabilization, and formulation of liquid protein pharmaceuticals. , 1999, International journal of pharmaceutics.

[16]  M. Brenner,et al.  Experimental characterization of electrospinning: the electrically forced jet and instabilities , 2001 .

[17]  B. Hsiao,et al.  Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA-PEG block copolymers. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[18]  Andreas Greiner,et al.  Compound Core–Shell Polymer Nanofibers by Co‐Electrospinning , 2003 .

[19]  Miss A.O. Penney.  (b) , 1974, The New Yale Book of Quotations.

[20]  J. Sohier,et al.  A novel method to obtain protein release from porous polymer scaffolds: emulsion coating. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[21]  G. Wnek,et al.  Two-Phase Electrospinning from a Single Electrified Jet: Microencapsulation of Aqueous Reservoirs in Poly(ethylene-co-vinyl acetate) Fibers , 2003 .

[22]  Lonnie D Shea,et al.  Controlled release systems for DNA delivery. , 2004, Molecular therapy : the journal of the American Society of Gene Therapy.

[23]  John B. Shoven,et al.  I , Edinburgh Medical and Surgical Journal.

[24]  Darrell H. Reneker,et al.  Bending instability of electrically charged liquid jets of polymer solutions in electrospinning , 2000 .

[25]  David J. Mooney,et al.  Polymeric Growth Factor Delivery Strategies for Tissue Engineering , 2003, Pharmaceutical Research.