Green synthesis and fabrication of an electrochemical and colorimetric sensor based on self-assembled peptide-Au nanofibril architecture

Abstract Owing to the excellent self-assembly properties and chemical flexibility, amyloid peptide were employed to build multifunctional architectures. Herein, we demonstrated the self-assembly of gold nanoparticles (AuNPs) in assistant of a sequence-designed peptide. As both surface ligands and self-assembly scaffolds of AuNPs, the formation of amyloid peptide nanofibrils could drive the self-assembly of AuNPs to form a fibrous hybrid architecture, which exhibits excellent electrochemical and colorimetric sensing performances. As a result, the electrochemical sensing towards H 2 O 2 achieved a linear response range of from 0.1 to 25.85 mM (R 2  = 0.997), and the detection limit was calculated to be 3.43 μM (S/N = 3). This self-assembled nanofiber was also employed for Hg 2+ detection. The sensor produced a color change which can readout with naked eye and UV–visual spectroscopy, with a linear detection range of 10–70 μM of Hg 2+ ion sample (R 2  = 0.991).

[1]  E. Placidi,et al.  Self-assembly of a model peptide incorporating a hexa-histidine sequence attached to an oligo-alanine sequence, and binding to gold NTA/nickel nanoparticles. , 2014, Biomacromolecules.

[2]  C. Alemán,et al.  Self-Assembly of Tetraphenylalanine Peptides. , 2015, Chemistry.

[3]  Mi Zhou,et al.  Self-assembled peptide-based hydrogels as scaffolds for anchorage-dependent cells. , 2009, Biomaterials.

[4]  Yanlian Yang,et al.  Nanomaterials for Reducing Amyloid Cytotoxicity , 2013, Advanced materials.

[5]  Yuliang Zhao,et al.  Serial silver clusters biomineralized by one peptide. , 2011, ACS nano.

[6]  Sreenath Bolisetty,et al.  Amyloid-carbon hybrid membranes for universal water purification. , 2016, Nature nanotechnology.

[7]  Sarit S. Agasti,et al.  Gold nanoparticles in chemical and biological sensing. , 2012, Chemical reviews.

[8]  Longhua Guo,et al.  Oriented gold nanoparticle aggregation for colorimetric sensors with surprisingly high analytical figures of merit. , 2013, Journal of the American Chemical Society.

[9]  W. Li,et al.  Bifunctional peptides that precisely biomineralize Au clusters and specifically stain cell nuclei. , 2012, Chemical communications.

[10]  M. Zhang,et al.  Colorimetric assay for parallel detection of Cd2+, Ni2+ and Co2+ using peptide-modified gold nanoparticles. , 2012, The Analyst.

[11]  Z. Chai,et al.  Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunsorbant Assay of Integrin Expression Level on Cell Membrane. , 2015, ACS nano.

[12]  Zhiqiang Su,et al.  Self-assembling peptide and protein amyloids: from structure to tailored function in nanotechnology. , 2017, Chemical Society reviews.

[13]  R. Mezzenga,et al.  New biocompatible thermo-reversible hydrogels from PNiPAM-decorated amyloid fibrils. , 2011, Chemical communications.

[14]  Yucheng Ding,et al.  One-Dimensional Au-ZnO Heteronanostructures for Ultraviolet Light Detectors by a Two-Step Dielectrophoretic Assembly Method. , 2015, ACS applied materials & interfaces.

[15]  R. Mezzenga,et al.  Universal behavior in the mesoscale properties of amyloid fibrils. , 2014, Physical review letters.

[16]  Gang Wei,et al.  When biomolecules meet graphene: from molecular level interactions to material design and applications. , 2016, Nanoscale.

[17]  R. Jin,et al.  On the ligand's role in the fluorescence of gold nanoclusters. , 2010, Nano letters.

[18]  R. Jin,et al.  Emergence of hierarchical structural complexities in nanoparticles and their assembly , 2016, Science.

[19]  C. Mirkin,et al.  Colorimetric Cu(2+) detection using DNA-modified gold-nanoparticle aggregates as probes and click chemistry. , 2010, Small.

[20]  Sarah L. Sewell,et al.  Biomimetic Synthesis of Titanium Dioxide Utilizing the R5 Peptide Derived from Cylindrotheca fusiformis , 2006 .

[21]  George C Schatz,et al.  What controls the melting properties of DNA-linked gold nanoparticle assemblies? , 2000, Journal of the American Chemical Society.

[22]  Zhiqiang Su,et al.  Synthesis and sensor applications of MoS2-based nanocomposites. , 2015, Nanoscale.

[23]  Xiaodong Chen,et al.  Colorimetric detection of mercury ions based on plasmonic nanoparticles. , 2013, Small.

[24]  Nathaniel L Rosi,et al.  Peptide-based methods for the preparation of nanostructured inorganic materials. , 2010, Angewandte Chemie.

[25]  G. Nienhaus,et al.  Supramolecular Self-Assembly Bioinspired Synthesis of Luminescent Gold Nanocluster-Embedded Peptide Nanofibers for Temperature Sensing and Cellular Imaging. , 2017, Bioconjugate chemistry.

[26]  Gang Wei,et al.  Design, fabrication, and biomedical applications of bioinspired peptide-inorganic nanomaterial hybrids. , 2017, Journal of materials chemistry. B.

[27]  R. Mezzenga,et al.  Hybrid Nanocomposites of Gold Single‐Crystal Platelets and Amyloid Fibrils with Tunable Fluorescence, Conductivity, and Sensing Properties , 2013, Advanced materials.

[28]  B. Nilsson,et al.  Review self‐assembly of amphipathic β‐sheet peptides: Insights and applications , 2012, Biopolymers.

[29]  T. Huber,et al.  Acidic peptides acting as growth modifiers of calcite crystals. , 2004, Chemical communications.

[30]  G. Nienhaus,et al.  Motif‐Designed Peptide Nanofibers Decorated with Graphene Quantum Dots for Simultaneous Targeting and Imaging of Tumor Cells , 2015 .

[31]  M. Morris,et al.  PEGylated gold nanoparticles: polymer quantification as a function of PEG lengths and nanoparticle dimensions , 2013 .

[32]  A. Singh,et al.  Retention of Enzymatic Activity of α-Amylase in the Reductive Synthesis of Gold Nanoparticles , 2007 .

[33]  D. Astruc,et al.  Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. , 2004, Chemical reviews.

[34]  D. Haynie,et al.  Protein- and peptide-based electrospun nanofibers in medical biomaterials. , 2012, Nanomedicine : nanotechnology, biology, and medicine.

[35]  Jaesung Park,et al.  Graphene‐Encapsulated Nanoparticle‐Based Biosensor for the Selective Detection of Cancer Biomarkers , 2011, Advanced materials.

[36]  Hyungjun Kim,et al.  Chiral arrangement of achiral Au nanoparticles by supramolecular assembly of helical nanofiber templates. , 2014, Journal of the American Chemical Society.

[37]  Shen-ming Chen,et al.  One-Pot Green Synthesis of Graphene Nanosheets Encapsulated Gold Nanoparticles for Sensitive and Selective Detection of Dopamine , 2017, Scientific Reports.

[38]  Gang Wei,et al.  Sequence‐Designed Peptide Nanofibers Bridged Conjugation of Graphene Quantum Dots with Graphene Oxide for High Performance Electrochemical Hydrogen Peroxide Biosensor , 2017 .

[39]  M. Mazzotti,et al.  Amyloid Templated Gold Aerogels , 2016, Advanced materials.

[40]  A. Belcher,et al.  Probing the interface between biomolecules and inorganic materials using yeast surface display and genetic engineering. , 2005, Acta biomaterialia.

[41]  Weiwei Wang,et al.  Facile colorimetric detection of Hg2+ based on anti-aggregation of silver nanoparticles. , 2014, Biosensors & bioelectronics.

[42]  Xiao Kuang,et al.  Electrostatic Assembly of Peptide Nanofiber-Biomimetic Silver Nanowires onto Graphene for Electrochemical Sensors. , 2014, ACS macro letters.

[43]  Jianping Xie,et al.  Protein-directed synthesis of highly fluorescent gold nanoclusters. , 2009, Journal of the American Chemical Society.

[44]  Gang Wei,et al.  Self-assembled peptide nanofibers on graphene oxide as a novel nanohybrid for biomimetic mineralization of hydroxyapatite , 2015 .

[45]  Anne-Kathrin Born,et al.  Amyloid‐Hydroxyapatite Bone Biomimetic Composites , 2014, Advanced materials.

[46]  Fang‐Xing Xiao.  Layer-by-Layer Self-Assembly Construction of Highly Ordered Metal-TiO2 Nanotube Arrays Heterostructures (M/TNTs, M = Au, Ag, Pt) with Tunable Catalytic Activities , 2012 .

[47]  Zhiqiang Su,et al.  Fabrication technologies and sensing applications of graphene-based composite films: Advances and challenges. , 2017, Biosensors & bioelectronics.

[48]  Mingwu Shen,et al.  PEGylated dendrimer-entrapped gold nanoparticles for in vivo blood pool and tumor imaging by computed tomography. , 2012, Biomaterials.

[49]  Gang Wei,et al.  Bottom-Up Synthesis and Sensor Applications of Biomimetic Nanostructures , 2016, Materials.

[50]  K. Suslick,et al.  Gold nanoparticles encapsulated in porous carbon. , 2012, Chemical communications.

[51]  Shuyan Song,et al.  CeO2-encapsulated noble metal nanocatalysts: enhanced activity and stability for catalytic application , 2015 .

[52]  Jörg Reichert,et al.  Controlled assembly of protein-protected gold nanoparticles on noncovalent functionalized carbon nanotubes , 2010 .