Engineering mammalian cells for solid-state sensor applications.

A fundamental advance in the development and application of cell- and tissue-based biosensors would be the ability to achieve air-dry stabilization of mammalian (especially human) cells with subsequent recovery following rehydration. The would allow for the preparation of sensors with extended shelf lives, only requiring the addition of water for activation. By understanding and subsequently employing the tactics used by desiccation-tolerant extremophiles, it may be possible to design stabilized mammalian cell-based biosensors. The approaches required to realize this goal are discussed and illustrated with several examples.

[1]  A. Covarrubias,et al.  Highly Hydrophilic Proteins in Prokaryotes and Eukaryotes Are Common during Conditions of Water Deficit* , 2000, The Journal of Biological Chemistry.

[2]  R. Helm,et al.  Structural Characterization of the Released Polysaccharide of Desiccation-Tolerant Nostoc communeDRH-1 , 2000, Journal of bacteriology.

[3]  R. Doolittle,et al.  A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.

[4]  M. Potts.  Desiccation tolerance of prokaryotes , 1994, Microbiological reviews.

[5]  A. Tunnacliffe,et al.  Anhydrobiotic Engineering of Gram-Negative Bacteria , 2000, Applied and Environmental Microbiology.

[6]  V. Mattimore,et al.  Radioresistance of Deinococcus radiodurans: functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation , 1996, Journal of bacteriology.

[7]  Mehmet Toner,et al.  Intracellular trehalose improves the survival of cryopreserved mammalian cells , 2000, Nature Biotechnology.

[8]  F. Plum.  Handbook of Physiology. , 1960 .

[9]  Don Paul Kovarcik,et al.  Active Fe-Containing Superoxide Dismutase and Abundant sodF mRNA in Nostoc commune(Cyanobacteria) after Years of Desiccation , 2000, Journal of bacteriology.

[10]  M. Potts,et al.  Chapter 13 – Life Without Water: Responses of Prokaryotes to Desiccation , 2000 .

[11]  K. Storey,et al.  Environmental stressors and gene responses , 2000 .

[12]  K M Shaffer,et al.  Immobilization of neural cells in three-dimensional matrices for biosensor applications. , 2000, Biosensors & bioelectronics.

[13]  R. Helm,et al.  Engineering Desiccation Tolerance inEscherichia coli , 2000, Applied and Environmental Microbiology.

[14]  A. Tunnacliffe,et al.  Intracellular trehalose improves osmotolerance but not desiccation tolerance in mammalian cells , 2000, FEBS letters.

[15]  J. Carpenter,et al.  Anhydrobiosis: cellular adaptations to extreme dehydration. , 1997 .

[16]  F. Levine,et al.  Trehalose expression confers desiccation tolerance on human cells , 2000, Nature Biotechnology.

[17]  S. Hladun,et al.  Water stress proteins of Nostoc commune (Cyanobacteria) are secreted with UV-A/B-absorbing pigments and associate with 1,4-beta-D-xylanxylanohydrolase activity. , 1994, The Journal of biological chemistry.