论文信息 - Some Properties of Polyacrylamide Gels

Some Properties of Polyacrylamide Gels

GELS composed of cross-linked Polyacrylamide have been used extensively in gel permeation chromatography and as a stabilizing medium in the zone electrophoresis of proteins and nucleic acids. Both types of experiment rely on a molecular sieve mechanism which depends on the total gel concentration as well as the proportion of cross links. These parameters also effect other physical properties of the gel such as the elasticity, turbidity and tendency to swell in excess solvent.

E. G. Richards | C. J. Temple | C. Temple | E. Richards

[1] J. S. Fawcett,et al. Molecular-Sieve Chromatography of Proteins on Granulated Polyacrylamide Gels , 1966 .

[2] T. Laurent,et al. A theory of gel filtration and its exeperimental verification , 1964 .

[3] P. Flory. Principles of polymer chemistry , 1953 .

[4] M. L. White,et al. Diffusion in a crosslinked acrylamide polymer gel , 1961 .

[5] S. Hjertén,et al. Apparatus for large-scale preparative polyacrylamide gel electrophoresis. , 1969, Analytical biochemistry.

[6] A. G. Ogston,et al. The spaces in a uniform random suspension of fibres , 1958 .

[7] Charles Tanford,et al. Physical Chemistry of Macromolecules , 1961 .

[8] D Rodbard,et al. Unified theory for gel electrophoresis and gel filtration. , 1970, Proceedings of the National Academy of Sciences of the United States of America.