Evaluation of the possibility of hypothermic and subornorthomeric storage of placental mesenchymal stem cells in alginate carriers

Mesenchymal stem cells (MSCs) are actively used in regenerative medicine to treat neuropa -thies, diabetes mellitus, burns, ulcers, infertility, premature conditions are able to maintain metabolism at a level sufficient for survival. The morphological study of cells in alginate microspheres was not informative, because of the difficulty cells visualizing in a three-dimensional structure and the density of alginate. Rapid screening tests may include trypan blue staining and MTT test. Conclusions. Placental cells in alginate microsphers can be stored in subnormothermic (+20°C) conditions no more than 48 hours. Storage more than 48 hours in subnormothermic conditions, or 24 hours in hypothermic conditions leads to a sharp metabolic activity decreasing.

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