Effect of Artificial Oocyte Activation by Calcium Ionophore on One-Day-Old Unfertilized Oocytes after ICSI

Abstract: At our clinic, a successfully fertilized human oocyte is defined as one with two polar bodies and two pronuclei (PN) 18 h after ICSI, but when some oocytes do not form PN. Recently, a successful pregnancy and live birth following artificial oocyte activation (AOA; using Ca2+ ionophore) of ICSI-treated one-day-old unfertilized oocytes was reported. In this study, we performed AOA on PN-lacking unfertilized oocytes, 18 h after ICSI (defined as Day 1-AOA, since ICSI=Day 0). The abilities of these oocytes to form PN and develop were compared with those of oocytes fertilized by ICSI (defined as No-AOA). Piezo-ICSI was performed on 168 oocytes, 50 of which failed to fertilize (0PN) but were subsequently artificially activated. 2PN formation rate was significantly lower in Day 1-AOA, and the rates of 1PN and ≥4PN were significantly higher than in No-AOA. In Day 1-AOA, 8 oocytes formed 2PN, 6 zygotes underwent cleavage and 2 of them developed to the morula stage, but none formed blastocysts. The rates of cleavage, morula, and blastocyst formation were significantly lower in Day 1-AOA than in No-AOA. These results suggest that the AOA protocol has room for improvement in the activation of unfertilized oocytes 18 h after ICSI.

[1]  M. Montag,et al.  The benefit of artificial oocyte activation is dependent on the fertilization rate in a previous treatment cycle. , 2012, Reproductive biomedicine online.

[2]  Huan Shen,et al.  A live birth of activated one-day-old unfertilized oocyte for a patient who experienced repeatedly near-total fertilization failure after intracytoplasmic sperm injection. , 2012, Chinese medical journal.

[3]  K. Yanagida.  Complete fertilization failure in ICSI , 2008, Human Cell.

[4]  T. Kawarabayashi,et al.  A normal uterus communicating with a double cervix and the vagina: a müllerian anomaly without any present classification. , 2009, Fertility and sterility.

[5]  N. Yaegashi,et al.  Successful pregnancy after oocyte activation by a calcium ionophore for a patient with recurrent intracytoplasmic sperm injection failure, with an assessment of oocyte activation and sperm centrosomal function using bovine eggs. , 2009, Fertility and sterility.

[6]  H. Katayose,et al.  The present status of artificial oocyte activation in assisted reproductive technology , 2008, Reproductive medicine and biology.

[7]  Z. Suludere,et al.  Clinical pregnancies and livebirths achieved by intracytoplasmic injection of round headed acrosomeless spermatozoa with and without oocyte activation in familial globozoospermia: case report. , 2008, Asian journal of andrology.

[8]  J. Zackowski,et al.  Second meiotic nondisjunction is not increased in postovulatory aged murine oocytes fertilized in vitro , 1988, In Vitro Cellular & Developmental Biology.

[9]  H. Katayose,et al.  Successful pregnancy after ICSI with strontium oocyte activation in low rates of fertilization. , 2006, Reproductive biomedicine online.

[10]  Jing-He Tan,et al.  Cumulus Cells Accelerate Aging of Mouse Oocytes1 , 2005, Biology of reproduction.

[11]  R. Dwek,et al.  Alkylated Imino Sugars, Reversible Male Infertility-Inducing Agents, Do Not Affect the Genetic Integrity of Male Mouse Germ Cells During Short-Term Treatment Despite Induction of Sperm Deformities1 , 2005, Biology of reproduction.

[12]  Y. Kamiguchi,et al.  How Long Do Parthenogenetically Activated Mouse Oocytes Maintain the Ability to Accept Sperm Nuclei as a Genetic Partner? , 2005, Journal of Assisted Reproduction and Genetics.

[13]  J. Luciani,et al.  The microtubular cytoskeleton and chromosomes of unfertilized human oocytes aged in vitro , 1988, Human Genetics.

[14]  S. Mizuno,et al.  Pregnancy following chemical activation of oocytes in a couple with repeated failure of fertilization using ICSI: case report. , 2004, Human reproduction.

[15]  K. Sperling,et al.  Prematurely condensed human sperm chromosomes after in vitro fertilization (IVF) , 1986, Human Genetics.

[16]  E. Margalioth,et al.  Successful pregnancy and delivery after calcium ionophore oocyte activation in a normozoospermic patient with previous repeated failed fertilization after intracytoplasmic sperm injection. , 2003, Fertility and sterility.

[17]  K. Nakagawa,et al.  Effect of activation with Ca ionophore A23187 and puromycin on the development of human oocytes that failed to fertilize after intracytoplasmic sperm injection. , 2001, Fertility and sterility.

[18]  T. Terada,et al.  Effect of Oocyte Aging on Parthenogenetic Activation with Cycloheximide and Alteration of the Activity of Maturation Promoting Factor during Aging of Bovine Oocytes , 2000 .

[19]  C. Hermenegildo,et al.  Long-term effects of postovulatory aging of mouse oocytes on offspring: a two-generational study. , 1999, Biology of reproduction.

[20]  H. Katayose,et al.  Successful fertilization and pregnancy following ICSI and electrical oocyte activation. , 1999, Human reproduction.

[21]  P. De Sutter,et al.  Effect of post-ovulatory age and calcium in the injection medium on the male pronucleus formation and metaphase entry following injection of human spermatozoa into golden hamster oocytes. , 1999, Molecular human reproduction.

[22]  R Janssens,et al.  Timing of oocyte activation, pronucleus formation and cleavage in humans after intracytoplasmic sperm injection (ICSI) with testicular spermatozoa and after ICSI or in-vitro fertilization on sibling oocytes with ejaculated spermatozoa. , 1998, Human reproduction.

[23]  S. London,et al.  Postovulatory ageing of mouse oocytes in vivo and premature centromere separation and aneuploidy. , 1998, Biology of reproduction.

[24]  D. Payne,et al.  Aetiology of failed and abnormal fertilization after intracytoplasmic sperm injection. , 1995, Human reproduction.

[25]  H. Katayose,et al.  Intracytoplasmic sperm injection using immobilized or motile human spermatozoon. , 1995, Fertility and sterility.

[26]  D. Payne,et al.  Assessment of fertilization failure and abnormal fertilization after intracytoplasmic sperm injection (ICSI). , 1995, Reproduction, fertility, and development.

[27]  J. Gerhart,et al.  Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs , 1984, The Journal of cell biology.