Rapid Detection of Infestation of Apple Fruits by the Peach Fruit Moth, Carposina sasakii Matsumura, Larvae Using a 0.2-T Dedicated Magnetic Resonance Imaging Apparatus

Infestation of harvested apple fruits by the peach fruit moth (Carposina sasakii Matsumura) was studied using a dedicated magnetic resonance imaging (MRI) apparatus equipped with a 0.2-T permanent magnet. Infested holes on the three-dimensional (3-D) images tracked ecological movements of peach fruit moth larvae within the food fruits, and thus in their natural habitat. Sensitive short solenoid coil and surface coil detectors were devised to shorten measurement times. The short solenoid coil detected infestation holes at a rate of 6.4 s per image by the single-slice 2-D measurement. The multi-slice 2-D measurement provided six slice images of a fruit within 2 min taken by the two detectors. These results indicate that the 0.2-T MRI apparatus allows one to distinguish sound fruits from infested ones, and also as a means for plant protection and the preservation of natural ecological systems in foreign trade.

[1]  Axel Haase,et al.  Simultaneous measurement of water flow velocity and solute transport in xylem and phloem of adult plants of Ricinus communis over a daily time course by nuclear magnetic resonance spectrometry , 2001 .

[2]  G. Stutte,et al.  Bound versus Free Water in Dormant Apple Buds—A Theory for Endodormancy , 1991 .

[3]  Frank J Vergeldt,et al.  MRI of long-distance water transport: a comparison of the phloem and xylem flow characteristics and dynamics in poplar, castor bean, tomato and tobacco. , 2006, Plant, cell & environment.

[4]  M. McCarthy,et al.  Diamagnetic susceptibility changes in apple tissue after bruising , 1995 .

[5]  Shin Utsuzawa,et al.  Correlation between acoustic emission, water status and xylem embolism in pine wilt disease. , 2007, Tree physiology.

[6]  N. Ishida,et al.  Thawing of frozen vegetables observed by a small dedicated MRI for food research. , 2006, Magnetic resonance imaging.

[7]  M. Faust,et al.  Changes in water status in peach buds on induction, development and release from dormancy , 1998 .

[8]  Spatially resolved magnetic resonance : methods, materials, medicine, biology, rheology, geology, ecology, hardware , 1998 .

[9]  史雄 井原,et al.  小型磁気共鳴イメージング装置（MRI）によるリンゴ幼果に食入したモモシンクイガ幼虫の検出 , 2008 .

[10]  P. Barreiro,et al.  Prospects for the rapid detection of mealiness in apples by nondestructive NMR relaxometry , 2002 .

[11]  J. A. Chudek,et al.  Nuclear magnetic resonance microscopy as a non-invasive tool to study the development of lepidopteran pupae , 1995 .

[12]  S. Y. Wang,et al.  Non-destructive detection of watercore in apple with nuclear magnetic resonance imaging , 1988 .

[13]  J. Labavitch,et al.  Xylella fastidiosa Infection and Ethylene Exposure Result in Xylem and Water Movement Disruption in Grapevine Shoots1[OA] , 2006, Plant Physiology.

[14]  H. Pfeifer.  Principles of Nuclear Magnetic Resonance Microscopy , 1992 .

[15]  T. Haishi,et al.  Examination of the tissue water in cucumber fruit by small dedicated magnetic resonance imaging with a 1-T permanent magnet. , 2009 .

[16]  T Haishi,et al.  Development of a 1.0 T MR microscope using a Nd-Fe-B permanent magnet. , 2001, Magnetic resonance imaging.

[17]  G. Johnson,et al.  Observation of the Oxygen Diffusion Barrier in Soybean (Glycine max) Nodules with Magnetic Resonance Microscopy. , 1992, Plant physiology.

[18]  G. Gassner,et al.  Combined proton NMR imaging and spectral analysis of locust embryonic development. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[19]  A. Haase,et al.  Rapid NMR Imaging Using Low Flip-Angle Pulses , 2004 .

[20]  Mathias Hoehn,et al.  Investigation of insect morphology by MRI: assessment of spatial and temporal resolution. , 2002, Magnetic resonance imaging.

[21]  J. Sprent,et al.  An application of NMR microimaging to investigate nitrogen fixing root nodules. , 1997, Magnetic resonance imaging.

[22]  Y. Xia,et al.  A non-invasive measurement of phloem and xylem water flow in castor bean seedlings by nuclear magnetic resonance microimaging , 1997, Planta.

[23]  Lee T.-A.,et al.  Nippon Shokuhin Kagaku Kogaku Kaishi , 2012 .

[24]  C. J. Clark,et al.  Loss of watercore from `Fuji' apple observed by magnetic resonance imaging , 1998 .

[25]  C. J. Clark,et al.  In vivo magnetic resonance imaging of xylem vessel contents in woody lianas , 2003 .