Impact of pre‐analytical parameters on the measurement of circulating microparticles: towards standardization of protocol

Summary.  Background:  Microparticles (MP) are small vesicles of 0.1–1 μm, released in response to activation or apoptosis. Over the past decade, they received an increasing interest both as biomarkers and biovectors in coagulation, inflammation and cancer. Clinical studies were conducted to assess their contribution to the identification of patients at cardiovascular risk. However, among the limitation of such studies, pre‐analytical steps remains an important source of variability and artifacts in MP analysis.

[1]  B. Ferry,et al.  Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the assay. , 2011, Thrombosis research.

[2]  R. Bertina,et al.  Pre-analytical and analytical issues in the analysis of blood microparticles , 2010, Thrombosis and Haemostasis.

[3]  J. Freyssinet,et al.  Mechanisms of microparticle generation: on the trail of the mitochondrion! , 2010, Seminars in thrombosis and hemostasis.

[4]  J. Inal,et al.  Red cell PMVs, plasma membrane-derived vesicles calling out for standards. , 2010, Biochemical and biophysical research communications.

[5]  N. Lion,et al.  Pre-analytical and methodological challenges in red blood cell microparticle proteomics. , 2010, Talanta.

[6]  C. Vrints,et al.  Flow cytometric detection of endothelial microparticles (EMP): effects of centrifugation and storage alter with the phenotype studied. , 2010, Thrombosis research.

[7]  G. Lippi,et al.  Right or wrong sample received for coagulation testing? Tentative algorithms for detection of an incorrect type of sample , 2010, International journal of laboratory hematology.

[8]  D. Neuberg,et al.  Tumor-Derived Tissue FactorBearing Microparticles Are Associated With Venous Thromboembolic Events in Malignancy , 2009, Clinical Cancer Research.

[9]  José A López,et al.  Microparticles and thrombotic disease , 2009, Current opinion in hematology.

[10]  G. Yong,et al.  A rapid flow cytometric technique for the detection of platelet‐monocyte complexes, activated platelets and platelet‐derived microparticles , 2009, International journal of laboratory hematology.

[11]  J. Rak,et al.  Microvesicles: Messengers and mediators of tumor progression , 2009, Cell cycle.

[12]  P. Nuchnoi,et al.  The absolute counting of red cell‐derived microparticles with red cell bead by flow rate based assay , 2009, Cytometry. Part B, Clinical cytometry.

[13]  L. Arnaud,et al.  Standardization of platelet‐derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies? , 2009, Journal of thrombosis and haemostasis : JTH.

[14]  A. Ganser,et al.  Recovery and composition of microparticles after snap-freezing depends on thawing temperature , 2009, Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis.

[15]  F. Dignat-George,et al.  Circulating endothelial cells, microparticles and progenitors: key players towards the definition of vascular competence , 2008, Journal of cellular and molecular medicine.

[16]  N. Lion,et al.  Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools , 2008, Vox sanguinis.

[17]  A. Shet.  Characterizing blood microparticles: Technical aspects and challenges , 2008, Vascular health and risk management.

[18]  A. Enjeti,et al.  Detection and Measurement of Microparticles: An Evolving Research Tool for Vascular Biology , 2007, Seminars in thrombosis and hemostasis.

[19]  D. Pisetsky,et al.  The Role of Microparticles in Inflammation and Thrombosis , 2007, Scandinavian journal of immunology.

[20]  A. Piccin,et al.  Circulating microparticles: pathophysiology and clinical implications. , 2007, Blood reviews.

[21]  J. Freyssinet,et al.  Procoagulant Microparticles: Disrupting the Vascular Homeostasis Equation? , 2006, Arteriosclerosis, thrombosis, and vascular biology.

[22]  Giuseppe Lippi,et al.  Influence of the needle bore size on platelet count and routine coagulation testing , 2006, Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis.

[23]  G. Lippi,et al.  Quality and reliability of routine coagulation testing: can we trust that sample? , 2006, Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis.

[24]  Joyce Curvers,et al.  Effects of storage‐induced platelet microparticles on the initiation and propagation phase of blood coagulation , 2006, British journal of haematology.

[25]  A. Tedgui,et al.  Circulating microparticles: a potential prognostic marker for atherosclerotic vascular disease. , 2006, Hypertension.

[26]  G. Lippi,et al.  Preanalytical variability: the dark side of the moon in laboratory testing , 2006, Clinical chemistry and laboratory medicine.

[27]  M. Gelderman,et al.  Cell membrane microparticles in blood and blood products: potentially pathogenic agents and diagnostic markers. , 2006, Transfusion medicine reviews.

[28]  C. Schalkwijk,et al.  Citrate anticoagulation abolishes degranulation of polymorphonuclear cells and platelets and reduces oxidative stress during haemodialysis. , 2006, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.

[29]  J. Freyssinet,et al.  More on: measuring circulating cell‐derived microparticles , 2005, Journal of thrombosis and haemostasis : JTH.

[30]  G. Lippi,et al.  Preanalytical variability in laboratory testing: influence of the blood drawing technique , 2005, Clinical chemistry and laboratory medicine.

[31]  R. Nieuwland,et al.  Measuring circulating cell‐derived microparticles , 2004, Journal of thrombosis and haemostasis : JTH.

[32]  L. Hefler,et al.  To butterfly or to needle: the pilot phase. , 2004, Annals of internal medicine.

[33]  R. Nieuwland,et al.  Cellular microparticles: new players in the field of vascular disease? , 2004, European journal of clinical investigation.

[34]  N. Young,et al.  Elevated circulating endothelial membrane microparticles in paroxysmal nocturnal haemoglobinuria , 2004, British journal of haematology.

[35]  J. Philippé,et al.  Comparison of five different citrated tubes and their in vitro effects on platelet activation. , 2004, Clinical chemistry.

[36]  C. Hack,et al.  Human cell‐derived microparticles promote thrombus formation in vivo in a tissue factor‐dependent manner , 2003, Journal of thrombosis and haemostasis : JTH.

[37]  R. Hebbel,et al.  Sickle blood contains tissue factor-positive microparticles derived from endothelial cells and monocytes. , 2003, Blood.

[38]  Y. J. Lee,et al.  Optimized flow cytometric assay for the measurement of platelet microparticles in plasma: pre-analytic and analytic considerations , 2002, Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis.

[39]  B. Polack,et al.  Preanalytical Recommendations of the ‘Groupe d’Etude sur l’Hémostase et la Thrombose’ (GEHT) for Venous Blood Testing in Hemostasis Laboratories , 2001, Pathophysiology of Haemostasis and Thrombosis.

[40]  Semple,et al.  Preanalytical requirements for flow cytometric evaluation of platelet activation: choice of anticoagulant , 1999, Transfusion medicine.