Fabrication of a bio‐inspired beta‐Tricalcium phosphate/collagen scaffold based on ceramic stereolithography and gel casting for osteochondral tissue engineering

Purpose – The purpose of this paper is to fabricate and characterize osteochondral beta‐tricalcium phosphate/collagen scaffold with bio‐inspired design by ceramic stereolithography (CSL) and gel casting.Design/methodology/approach – Histological analysis was applied to explore the morphological characteristics of the transitional structure between the bone and the cartilage. The acquired data were used to design biomimetic biphasic scaffolds, which include the bone phase, cartilage phase, and their transitional structure. The engineered scaffolds were fabricated from β‐TCP‐collagen by CSL and gel casting. The cartilage phase was added to the ceramic phase by gel‐casting and freeze drying.Findings – The resulting ceramic scaffolds were composed of a bone phase with the following properties: 700‐900 μm pore size, 200‐500 μm interconnected pores size, 50‐65 percent porosity, fully interconnected, ∼12 Mpa compressive strength. A suitable binding force between cartilage phase and ceramic phase was achieved by ...

[1]  S M Giannitelli,et al.  Current trends in the design of scaffolds for computer-aided tissue engineering. , 2014, Acta biomaterialia.

[2]  Roger J. Narayan,et al.  Stereolithography in tissue engineering , 2014, Journal of Materials Science: Materials in Medicine.

[3]  Jinlong Yang,et al.  Recent developments in gelcasting of ceramics , 2011 .

[4]  F. Melchels,et al.  A review on stereolithography and its applications in biomedical engineering. , 2010, Biomaterials.

[5]  C. Seebach,et al.  Comparison of six bone-graft substitutes regarding to cell seeding efficiency, metabolism and growth behaviour of human mesenchymal stem cells (MSC) in vitro. , 2010, Injury.

[6]  Zhangwei Chen,et al.  Curing characteristics of ceramic stereolithography for an aqueous-based silica suspension , 2010 .

[7]  Dichen Li,et al.  A novel aqueous ceramic suspension for ceramic stereolithography , 2010 .

[8]  Dichen Li,et al.  Rapid casting of turbine blades with abnormal film cooling holes using integral ceramic casting molds , 2010 .

[9]  Dichen Li,et al.  Rapid fabrication of alumina-based ceramic cores for gas turbine blades by stereolithography and gelcasting , 2009 .

[10]  F. Melchels,et al.  A poly(D,L-lactide) resin for the preparation of tissueengineering scaffolds by stereolithography , 2009 .

[11]  Jan Feijen,et al.  A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography. , 2009, Biomaterials.

[12]  Dichen Li,et al.  Fabrication of integral ceramic mold for investment casting of hollow turbine blade based on stereolithography , 2009 .

[13]  Robert L Sah,et al.  Tissue engineering of articular cartilage with biomimetic zones. , 2009, Tissue engineering. Part B, Reviews.

[14]  Dong-Woo Cho,et al.  Development of nano- and microscale composite 3D scaffolds using PPF/DEF-HA and micro-stereolithography , 2009 .

[15]  Jan Feijen,et al.  Fumaric acid monoethyl ester-functionalized poly(D,L-lactide)/N-vinyl-2-pyrrolidone resins for the preparation of tissue engineering scaffolds by stereolithography. , 2009, Biomacromolecules.

[16]  S. Mc,et al.  Direct fabrication process of ceramics based on stereolithography , 2009 .

[17]  Liu Yaxiong,et al.  Preparation of chitosan-gelatin hybrid scaffolds with well-organized microstructures for hepatic tissue engineering. , 2009, Acta biomaterialia.

[18]  Ivan Martin,et al.  Design of graded biomimetic osteochondral composite scaffolds. , 2008, Biomaterials.

[19]  Dong-Woo Cho,et al.  Application of microstereolithography in the development of three-dimensional cartilage regeneration scaffolds , 2008, Biomedical microdevices.

[20]  W. Yeong,et al.  Engineering functionally graded tissue engineering scaffolds. , 2008, Journal of the mechanical behavior of biomedical materials.

[21]  Dichen Li,et al.  Fabrication of a calcium phosphate scaffold with a three dimensional channel network and its application to perfusion culture of stem cells , 2007 .

[22]  Michael J Yaszemski,et al.  Poly(propylene fumarate) bone tissue engineering scaffold fabrication using stereolithography: effects of resin formulations and laser parameters. , 2007, Biomacromolecules.

[23]  Eduardo Saiz,et al.  Freeze casting of hydroxyapatite scaffolds for bone tissue engineering. , 2006, Biomaterials.

[24]  Baojin Fu,et al.  Accumulated Chromosomal Instability in Murine Bone Marrow Mesenchymal Stem Cells Leads to Malignant Transformation , 2006, Stem cells.

[25]  A. Ogose,et al.  Histological assessment in grafts of highly purified beta-tricalcium phosphate (OSferion) in human bones. , 2006, Biomaterials.

[26]  Dichen Li,et al.  Design and fabrication of CAP scaffolds by indirect solid free form fabrication , 2005 .

[27]  G H van Lenthe,et al.  Synthesis and characterization of porous beta-tricalcium phosphate blocks. , 2005, Biomaterials.

[28]  John W. Halloran,et al.  Freeform Fabrication of Ceramics via Stereolithography , 2005 .

[29]  S. Hollister.  Porous scaffold design for tissue engineering , 2005, Nature materials.

[30]  C. E. Corcione,et al.  Laser stereolithography of ZrO 2 toughened Al 2 O 3 , 2004 .

[31]  C. E. Corcione,et al.  Laser stereolithography of ZrO2 toughened Al2O3 , 2004 .

[32]  R. Sah.  Interface and bulk regions in the repair, regeneration, and replacement of articular cartilage. , 2004, Journal of musculoskeletal & neuronal interactions.

[33]  M Bohner,et al.  Theoretical model to determine the effects of geometrical factors on the resorption of calcium phosphate bone substitutes. , 2004, Biomaterials.

[34]  Buddy D Ratner,et al.  Biomaterials: where we have been and where we are going. , 2004, Annual review of biomedical engineering.

[35]  Dietmar W Hutmacher,et al.  Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems. , 2004, Trends in biotechnology.

[36]  G. Ateshian,et al.  Anatomically shaped osteochondral constructs for articular cartilage repair. , 2003, Journal of biomechanics.

[37]  Malcolm N. Cooke,et al.  Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth. , 2003, Journal of biomedical materials research. Part B, Applied biomaterials.

[38]  S. Hollister,et al.  Optimal design and fabrication of scaffolds to mimic tissue properties and satisfy biological constraints. , 2002, Biomaterials.

[39]  Arnold I Caplan,et al.  Repair of osteochondral defect with tissue-engineered two-phase composite material of injectable calcium phosphate and hyaluronan sponge. , 2002, Tissue engineering.

[40]  Thierry Chartier,et al.  Stereolithography of structural complex ceramic parts , 2002 .

[41]  D. Hutmacher,et al.  Scaffold development using 3D printing with a starch-based polymer , 2002 .

[42]  Scott J Hollister,et al.  Mechanical and in vivo performance of hydroxyapatite implants with controlled architectures. , 2002, Biomaterials.

[43]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. , 2002, Tissue engineering.

[44]  Wei Sun,et al.  Recent development on computer aided tissue engineering - a review , 2002, Comput. Methods Programs Biomed..

[45]  H. Yoshikawa,et al.  Novel hydroxyapatite ceramics with an interconnective porous structure exhibit superior osteoconduction in vivo. , 2002, Journal of biomedical materials research.

[46]  R. Kandel,et al.  Porous calcium polyphosphate scaffolds for bone substitute applications -- in vitro characterization. , 2001, Biomaterials.

[47]  Thierry Chartier,et al.  Stereolithography for manufacturing ceramic parts , 2000 .

[48]  K. Hong,et al.  Osteoconduction at porous hydroxyapatite with various pore configurations. , 2000, Biomaterials.

[49]  F. Korkusuz,et al.  A novel osteochondral implant. , 1999, Biomaterials.

[50]  P. Ma,et al.  Porous poly(L-lactic acid)/apatite composites created by biomimetic process. , 1999, Journal of biomedical materials research.

[51]  J. Lu,et al.  Role of interconnections in porous bioceramics on bone recolonization in vitro and in vivo , 1999, Journal of materials science. Materials in medicine.

[52]  T. Chartier,et al.  Stereolithography for the fabrication of ceramic three‐ dimensional parts , 1998 .

[53]  S Z Zhong,et al.  Biomechanical characteristics of human trabecular bone. , 1997, Clinical biomechanics.

[54]  John W. Halloran,et al.  Stereolithography of ceramic suspensions , 1997 .

[55]  S A Goldstein,et al.  The relationship between the structural and orthogonal compressive properties of trabecular bone. , 1994, Journal of biomechanics.

[56]  P. Eggli,et al.  Porous hydroxyapatite and tricalcium phosphate cylinders with two different pore size ranges implanted in the cancellous bone of rabbits. A comparative histomorphometric and histologic study of bony ingrowth and implant substitution. , 1988, Clinical orthopaedics and related research.

[57]  T. Oegema,et al.  The effects of indirect blunt trauma on adult canine articular cartilage. , 1983, The Journal of bone and joint surgery. American volume.

[58]  P. Bullough,et al.  The vascularity and remodelling of subchondrial bone and calcified cartilage in adult human femoral and humeral heads. An age- and stress-related phenomenon. , 1977, Journal of Bone and Joint Surgery-british Volume.