Family 17 and 28 Carbohydrate-Binding Modules Discriminated Different Cell-Wall Sites in Sweet Potato Roots

Endoglucanase Cel5A from Clostridium josui contains a family 17 carbohydrate-binding module (CBM) (CjCBM17) and a family 28 CBM (CjCBM28) in tandem. These two CBMs bound to non-crystalline cellulose and β-1,3-1,4-glucan. Our results indicate that the CBMs recognized different components on the cell wall of a sweet potato root. The root was cut into longitudinal sections. We used CjCBM17 and CjCBM28 fused to two different fluorescent proteins to visualize differential recognition of the plant cell wall. When they were microscopically observed, CjCBM28-fused cyan fluorescent protein (CFP) differentially bound to the root cap, but CjCBM17-fused blue fluorescent protein (BFP) did not. CjCBM17-BFP bound to the central part or the root apical meristem. These results suggest that CjCBM17 and CjCBM28 recognize different sites of the cell wall and that the cell-wall components and the polysaccharides configuration in the cell wall differ between tissues.

[1]  S. Karita,et al.  Characterization of Family 17 and Family 28 Carbohydrate-Binding Modules from Clostridium josui Cel5A , 2009, Bioscience, biotechnology, and biochemistry.

[2]  M. Pauly,et al.  Inducible expression of Pisum sativum xyloglucan fucosyltransferase in the pea root cap meristem, and effects of antisense mRNA expression on root cap cell wall structural integrity , 2008, Plant Cell Reports.

[3]  K. Keegstra,et al.  Biosynthesis of plant cell wall polysaccharides - a complex process. , 2006, Current opinion in plant biology.

[4]  D. Bolam,et al.  Understanding the Biological Rationale for the Diversity of Cellulose-directed Carbohydrate-binding Modules in Prokaryotic Enzymes* , 2006, Journal of Biological Chemistry.

[5]  D. Bolam,et al.  Differential recognition of plant cell walls by microbial xylan-specific carbohydrate-binding modules. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[6]  D. Bolam,et al.  Glycoside hydrolase carbohydrate-binding modules as molecular probes for the analysis of plant cell wall polymers. , 2004, Analytical biochemistry.

[7]  G. Daniel,et al.  Use of a fluorescence labelled, carbohydrate-binding module from Phanerochaete chrysosporium Cel7D for studying wood cell wall ultrastructure , 2003, Biotechnology Letters.

[8]  D. Kilburn,et al.  Recognition and Hydrolysis of Noncrystalline Cellulose* , 2003, The Journal of Biological Chemistry.

[9]  Clare G. Steele-King,et al.  Cell wall pectic (1-->4)-beta-d-galactan marks the acceleration of cell elongation in the Arabidopsis seedling root meristem. , 2003, The Plant journal : for cell and molecular biology.

[10]  P. Simpson,et al.  Pseudomonas cellulose-binding domains mediate their effects by increasing enzyme substrate proximity. , 1998, The Biochemical journal.

[11]  S. Kitoh,et al.  Events associated with the rapid separation of cells from detached roots of Azolla filiculoides depend on pH , 1994 .

[12]  B Henrissat,et al.  A classification of glycosyl hydrolases based on amino acid sequence similarities. , 1991, The Biochemical journal.

[13]  D. Northcote,et al.  The relationship of root-cap slimes to proteins , 1974 .

[14]  D. Kilburn,et al.  Identification and glucan-binding properties of a new carbohydrate-binding module family. , 2002, The Biochemical journal.

[15]  E. Karlsson,et al.  Carbohydrate-binding modules from a thermostable Rhodothermus marinus xylanase: cloning, expression and binding studies. , 2000, The Biochemical journal.

[16]  K. Keegstra,et al.  The Structure of Plant Cell Walls: III. A Model of the Walls of Suspension-cultured Sycamore Cells Based on the Interconnections of the Macromolecular Components. , 1973, Plant physiology.