Analysis of cytokines in the peritoneal fluid of endometriosis patients as a function of the menstrual cycle stage using the Bio-Plex® platform

Objective: Endometriosis is a painful disease affecting 10–15% of reproductive-age women. Concentrations of several cytokines and angiogenic factors in peritoneal fluid (PF) have been found to correlate with the severity of the disease. However, levels of some analytes vary across the menstrual cycle, and an ideal biomarker of endometriosis has not yet been identified. We have compared the PF concentrations of different cytokines in proliferative and secretory phases in women with and without the disease using the Bio-Plex platform. Methods: PF was aspirated during laparoscopy (N = 133) and the PF concentrations of 18 cytokines from Bio-Plex panels I and II determined with the serum protocol. Results: Increased PF concentrations of IL-6, IL-18, eotaxin, and MCP-1 were found in endometriosis with no changes with menstrual cycle. Levels of IL-12(p70), ICAM-1, and GRO-α were higher in the secretory phase, while eotaxin concentrations were lower. Conclusion: Of the 18 cytokines tested, IL-6, IL-18 and MCP-1 were the best PF markers of endometriosis.

[1]  M. Nishida,et al.  Role of chemokines in the pathogenesis of endometriosis. , 2011, Frontiers in bioscience.

[2]  C. Hernández-Guerrero,et al.  Quantitative and qualitative peritoneal immune profiles, T‐cell apoptosis and oxidative stress‐associated characteristics in women with minimal and mild endometriosis , 2011, BJOG : an international journal of obstetrics and gynaecology.

[3]  B. McKinnon,et al.  Pain Symptoms and Peritoneal Fluid Cytokine and Marker Concentrations in Women with and without Endometriosis , 2009 .

[4]  P. Luciw,et al.  A comparison of multiplex suspension array large‐panel kits for profiling cytokines and chemokines in rheumatoid arthritis patients , 2009, Cytometry. Part B, Clinical cytometry.

[5]  A. Agarwal,et al.  Serum and peritoneal abnormalities in endometriosis: potential use as diagnostic markers. , 2006, Minerva ginecologica.

[6]  E. Salamalekis,et al.  Immunological factors and their role in the genesis and development of endometriosis 
ARTICLE HAS BEEN RETRACTED , 2006, The journal of obstetrics and gynaecology research.

[7]  Carole Foy,et al.  A comparability study of the emerging protein array platforms with established ELISA procedures. , 2005, Journal of immunological methods.

[8]  Robert N. Taylor,et al.  Epithelial neutrophil-activating peptide 78 concentrations are elevated in the peritoneal fluid of women with endometriosis. , 2003, Fertility and sterility.

[9]  C. Calhaz-Jorge,et al.  Peritoneal fluid concentrations of interleukin-8 in patients with endometriosis depend on the severity of the disorder and are higher in the luteal phase. , 2003, Human reproduction.

[10]  Howard M Shapiro,et al.  Report from a workshop on multianalyte microsphere assays. , 2002, Cytometry.

[11]  Y. Taketani,et al.  Concentrations of interferon-gamma-induced protein-10 (IP-10), an antiangiogenic substance, are decreased in peritoneal fluid of women with advanced endometriosis. , 2002, American journal of reproductive immunology.

[12]  A. Agarwal,et al.  Prediction of endometriosis with serum and peritoneal fluid markers: a prospective controlled trial. , 2002, Human reproduction.

[13]  Gruppo italiano per lo studio dell'endometriosi.  Relationship between stage, site and morphological characteristics of pelvic endometriosis and pain. , 2001, Human reproduction.

[14]  A. Rapkin,et al.  Peritoneal fluid interleukin-6 in women with chronic pelvic pain. , 2000, Fertility and sterility.

[15]  D. Wallwiener,et al.  Localization in tissues and secretion of eotaxin by cells from normal endometrium and endometriosis. , 2000, The Journal of clinical endocrinology and metabolism.

[16]  N. Terakawa,et al.  Role of Cytokines in Progression of Endometriosis , 1999, Gynecologic and Obstetric Investigation.

[17]  M. Nisolle,et al.  Vascular endothelial growth factor (VEGF) in endometriosis. , 1998, Human reproduction.

[18]  M. Nisolle,et al.  Peritoneal endometriosis, ovarian endometriosis, and adenomyotic nodules of the rectovaginal septum are three different entities. , 1997, Fertility and sterility.

[19]  M. Canis,et al.  Revised American Society for Reproductive Medicine classification of endometriosis: 1996. , 1997, Fertility and sterility.

[20]  S. McColl,et al.  Elevated concentration and biologic activity of monocyte chemotactic protein-1 in the peritoneal fluid of patients with endometriosis. , 1996, Fertility and sterility.

[21]  J. Punnonen,et al.  Increased levels of interleukin-6 and interleukin-10 in the peritoneal fluid of patients with endometriosis. , 1996, American journal of obstetrics and gynecology.

[22]  J. Tseng,et al.  Interleukin-8 concentrations are elevated in peritoneal fluid of women with endometriosis. , 1995, Fertility and sterility.

[23]  M. Nisolle,et al.  Rectovaginal septum, endometriosis or adenomyosis: laparoscopic management in a series of 231 patients. , 1995, Human reproduction.

[24]  R. Pardi,et al.  Expression of Intercellular Adhesion Molecule‐1 (ICAM‐1) on Cultured Human Endometrial Stromal Cells and Its Role in the Interaction With Natural Killers , 1994, American journal of reproductive immunology.

[25]  T. Schall,et al.  Peritoneal fluid concentrations of the cytokine RANTES correlate with the severity of endometriosis. , 1993, American journal of obstetrics and gynecology.

[26]  M. Nisolle,et al.  Morphometric study of the stromal vascularization in peritoneal endometriosis. , 1993, Fertility and sterility.

[27]  P. Jap,et al.  Endometrial epithelial cells in peritoneal fluid during the early follicular phase. , 1991, Fertility and sterility.

[28]  J. Lee,et al.  Interleukin-1: a possible role in the infertility associated with endometriosis. , 1987, Fertility and sterility.

[29]  M G Hammond,et al.  Retrograde menstruation in healthy women and in patients with endometriosis. , 1984, Obstetrics and gynecology.

[30]  J. Sampson.  Metastatic or Embolic Endometriosis, due to the Menstrual Dissemination of Endometrial Tissue into the Venous Circulation. , 1927, The American journal of pathology.

[31]  R. Albulescu,et al.  Correlation of XMAP and ELISA cytokine profiles; development and validation for immunotoxicological studies in vitro. , 2010, Roumanian archives of microbiology and immunology.

[32]  P. Vercellini,et al.  Association between endometriosis stage, lesion type, patient characteristics and severity of pelvic pain symptoms: a multivariate analysis of over 1000 patients. , 2007, Human reproduction.

[33]  T. Tamaya,et al.  [Angiogenic factors in endometriosis]. , 2001, Nihon rinsho. Japanese journal of clinical medicine.

[34]  M. Mueller,et al.  Immunobiology of endometriosis. , 2001, Fertility and sterility.

[35]  M. Franchi,et al.  Estroprogestin vs. gonadotrophin agonists plus estroprogestin in the treatment of endometriosis-related pelvic pain: a randomized trial. Gruppo Italiano per lo Studio dell'Endometriosi. , 2000, European journal of obstetrics, gynecology, and reproductive biology.

[36]  R. Schenken,et al.  Whole explants of peritoneum and endometrium: a novel model of the early endometriosis lesion. , 1999, Fertility and sterility.

[37]  S. Tazuke,et al.  Interleukin-8 concentration in peritoneal fluid of patients with endometriosis and modulation of interleukin-8 expression in human mesothelial cells. , 1996, Molecular human reproduction.