PCR‐linked in vitro expression: a novel system for high‐throughput construction and screening of protein libraries

A novel entirely in vitro strategy for generation and screening of a combinatorial protein library in an array format has been developed and is experimentally demonstrated. The strategy exploits virtues of PCR and in vitro coupled transcription/translation. Our new approach provides high‐throughput construction and screening of the in vitro protein library, and compatibility with various selection methods.

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