1H NMR plasma metabolomic profiling of ovarian quiescence in energy balanced postpartum dairy cows

Abstract Background: As the milk production of dairy cows increases, the reproductive capacity gradually declines. Ovarian quiescence has become one of the concerns of the dairy industry. Objective: To explore the different plasma metabolite levels between estrus and anestrus in energy balanced (EB) dairy cows. Methods: Ten estrous and 10 anestrus EB Holstein cows in early lactation were selected for the study. 1H nuclear magnetic resonance technology was used to detect plasma metabolites and screen different plasma metabolites between anestrous and estrous cows at 60–90 days postpartum using multivariate statistical analysis. Results: Within an elective waiting period of 60−90 days postpartum mean plasma concentration of total estrogens was significantly higher in estrus cows as compared to anestrus cows (71.2 ± 26.0 and 42.4 ± 16.7 pg/mL, respectively). Seven plasma metabolites (isoleucine, leucine, valine, alanine, arginine, choline and phosphatecholine) demonstrated significant decreases in estrous dairy cows relative to anestrous subjects. The main pathway was leucine, isoleucine and valine biosynthesis. Conclusion: Anestrus in dairy cows is accompanied by alterations in amino acid, glucose and lipid metabolism based on 1H NMR analysis.

[1]  R. Vaughan,et al.  BCAA Metabolism and Insulin Sensitivity - Dysregulated by Metabolic Status? , 2018, Molecular nutrition & food research.

[2]  D. Mohanty,et al.  Certain hormonal profiles of postpartum anestrus jersey crossbred cows treated with controlled internal drug release and ovsynch protocol , 2016, Veterinary world.

[3]  S. Shu,et al.  Metabolic profiles using (1)H-nuclear magnetic resonance spectroscopy in postpartum dairy cows with ovarian inactivity. , 2016, Theriogenology.

[4]  Jiasan Zheng,et al.  1H-Nuclear Magnetic Resonance-Based Plasma Metabolic Profiling of Dairy Cows with Fatty Liver , 2015, Asian-Australasian journal of animal sciences.

[5]  J. Colca,et al.  Loss of Mitochondrial Pyruvate Carrier 2 in the Liver Leads to Defects in Gluconeogenesis and Compensation via Pyruvate-Alanine Cycling. , 2015, Cell metabolism.

[6]  Ming-hui Li,et al.  Insight into biological system responses in goldfish (Carassius auratus) to multiple doses of avermectin exposure by integrated 1H NMR-based metabolomics , 2015 .

[7]  David S. Wishart,et al.  MetaboAnalyst 2.0—a comprehensive server for metabolomic data analysis , 2012, Nucleic Acids Res..

[8]  A. Evans,et al.  A review of the causes of poor fertility in high milk producing dairy cows , 2010, Animal Reproduction Science.

[9]  A. Ramírez de Molina,et al.  Differential Role of Human Choline Kinase α and β Enzymes in Lipid Metabolism: Implications in Cancer Onset and Treatment , 2009, PloS one.

[10]  David S. Wishart,et al.  MetaboAnalyst: a web server for metabolomic data analysis and interpretation , 2009, Nucleic Acids Res..

[11]  W. Butler.  Relationships of Negative Energy Balance with Fertility , 2005 .

[12]  A. Ramírez de Molina,et al.  Phospholipase D and choline kinase: their role in cancer development and their potential as drug targets. , 2003, Progress in cell cycle research.

[13]  C. Knauf,et al.  Involvement of NPY Y2 receptor subtype in the control of the spontaneous NO/GnRH release at the rat median eminence , 2001, Neuroreport.

[14]  B. Veyret,et al.  l-Arginine Availability Modulates Local Nitric Oxide Production and Parasite Killing in Experimental Trypanosomiasis , 2000, Infection and Immunity.

[15]  D. J. Ambrose,et al.  Clinical and research applications of real-time ultrasonography in bovine reproduction: a review. , 1994, The Canadian veterinary journal = La revue veterinaire canadienne.