An economical method of simple sequence repeat(SSR) detection was used in studies on identification of 26 apple cultivars in which fluorescent labeling of SSR-PCR products was used,with tailed primer M13(TP-M13-SSR).5 SSR mark-ers primers amplified 52 alleles,with an average of 10.4 alleles per locus.The primer CH04h02 could distinguish all of the apple cultivars.The ranges of gene diversity,PIC and locus heterozygosity on 5 SSR locuses were 0.6620～0.9149,0.6326～ 0.9086 and 0.6538～1.0000,respectively.The method has the advantages of high-throughput,sensitiveness,cost-effective-ness and high accuracy.And it have been used in studies on genetic diversity analysis of apple successfully.