Heat shock proteins and thermotolerance; a comparison of induction kinetics.

The hypothesis that the expression of heat shock proteins following a preliminary hyperthermic treatment is responsible for subsequent thermotolerance to a second heat treatment is examined. CHO cells were given a 12 min, 45 degrees C pretreatment and then incubated for varying intervals at 37 degrees C. The synthesis of certain intracellular proteins was monitored as a function of time post-incubation by using 35S-methionine incorporation as determined in SDS polyacrylamide gel electrophoresis. Cell survival was concurrently measured by challenging the cells with a second heat treatment (45 degrees C/27 min). Major heat shock proteins were observed at 68 000, 89 000 and 110 000 daltons. The synthesis of these proteins was significantly reduced in the presence of cyclohexamide. The total 35S-methionine incorporation into these proteins correlated well with the induction of survival resistance (thermotolerance). An approximate exponential relationship between survival and the amount of each of these proteins may occur. These and other heat shock proteins were also present, in a significantly reduced degree, in control (non-heat shocked) cells maintained under normal culture conditions at 37 degrees C. It is possible that heat shock proteins are responsible for the phenomenon of thermotolerance.

[1]  S. Matsui,et al.  Isopeptidase: a novel eukaryotic enzyme that cleaves isopeptide bonds. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[2]  R. Johnson,et al.  Heat shock proteins and biological response to hyperthermia. , 1982, The British journal of cancer. Supplement.

[3]  S. Elliott,et al.  Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae , 1982, Molecular and cellular biology.

[4]  W. Pledger,et al.  Platelet-derived growth factor-modulated proteins: constitutive synthesis by a transformed cell line. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[5]  R. Cousins,et al.  Hormonal Regulation of Liver Metallothionein Zinc: Independent and Synergistic Action of Glucagon and Glucocorticoids 1 , 1981, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[6]  R. Gomer,et al.  Heat shock proteins are methylated in avian and mammalian cells. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[7]  M. Webb,et al.  Metabolism of zinc and copper in the neonate. (Zinc, copper)-thionein in the developing rat kidney and testis. , 1981, The Journal of biological chemistry.

[8]  Amy S. Lee,et al.  The accumulation of three specific proteins related to glucose‐regulated proteins in a temperature‐sensitive hamster mutant cell line K12 , 1981, Journal of cellular physiology.

[9]  J. Jackson,et al.  Amino acid deprivation induces synthesis of four proteins in chick embryo cells , 1980 .

[10]  M. Scott,et al.  Translational control of protein synthesis in response to heat shock in D. melanogaster cells , 1980, Cell.

[11]  G. Hahn,et al.  A proposed operational model of thermotolerance based on effects of nutrients and the initial treatment temperature. , 1980, Cancer research.

[12]  M. Karin,et al.  Metallothionein mRNA induction in HeLa cells in response to zinc or dexamethasone is a primary induction response , 1980, Nature.

[13]  J. Celis,et al.  A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells , 1980, The Journal of cell biology.

[14]  H. Oppermann,et al.  Transition series metals and sulfhydryl reagents induce the synthesis of four proteins in eukaryotic cells. , 1980, Biochimica et biophysica acta.

[15]  F. Amalric,et al.  Effects of heat shock on gene expression and subcellular protein distribution in Chinese hamster ovary cells. , 1979, Nucleic acids research.

[16]  J. Subjeck,et al.  Radiation and hyperthermia. , 1979, Bulletin of the New York Academy of Medicine.

[17]  K. Marotti,et al.  A candidate rat-specific gene product of the Kirsten murine sarcoma virus. , 1979, Virology.

[18]  M. Ashburner,et al.  The induction of gene activity in drosophila by heat shock , 1979, Cell.

[19]  K. Henle,et al.  Heat fractionation and thermotolerance: a review. , 1978, Cancer research.

[20]  K. Henle,et al.  Interaction of hyperthermia and radiation in CHO cells: recovery kinetics. , 1976, Radiation research.

[21]  E. Rosenberg,et al.  Regulation of cyanide-insensitive respiration in Neurospora. , 1976, European journal of biochemistry.

[22]  E. Gerner,et al.  Induced thermal resistance in HeLa cells , 1975, Nature.

[23]  D. Bray,et al.  Peptide mapping of proteins from acrylamide gels. , 1973, Analytical biochemistry.

[24]  C. Heidelberger,et al.  Studies on the quantitative biology of hyperthermic killing of HeLa cells. , 1973, Cancer research.

[25]  M. Harris.  Growth and survival of mammalian cells under continuous thermal stress. , 1969, Experimental cell research.

[26]  B. Hille,et al.  ANALYSIS OF SOME TEMPERATURE EFFECTS ON DROSOPHILA PUPAE , 1966 .

[27]  T. Puck,et al.  Life cycle analysis of mammalian cells. 3. The inhibition of division in Chinese hamster cells by puromycin and actinomycin. , 1963, Biophysical journal.

[28]  R. Milkman.  Temperature Effects on Day Old Drosophila Pupae , 1962, The Journal of general physiology.

[29]  O. Selawry,et al.  Hyperthermia in tissue-cultured cells of malignant origin. , 1957, Cancer research.

[30]  H. K. Mitchell,et al.  Specific protection from phenocopy induction by heat shock , 1979 .

[31]  W. Dewey,et al.  Variation in sensitivity to heat shock during the cell-cycle of Chinese hamster cells in vitro. , 1971, International journal of radiation biology and related studies in physics, chemistry, and medicine.