Defined adhesion and growth of neurones on artificial structured substrates

Patterned adhesion of neurones and directed growth of neurites is the pre-requisite to establish meaningful in vitro models for the study of interactions and signalling between neurones. We have therefore studied two model systems for structured substrates. Silicon samples were microstructured by etching. Neurones adhered preferentially onto the bottom of the resulting grooves and wells. Outgrowth of neurites could be observed on adsorptive coating with polylysine and laminin. Most of the neurites did not cross the border between the plain surfaces of the silicon sample and the bottom of the grooves and wells. The second approach was to use interdigitating platinum comb electrodes. One of them was coated with laminin by electrochemical polymerisation. A strong outgrowth of axons was found, and such a surface modification appears to be suitable for the creation of neuronal patterns with effective growth of axons in vitro.

[1]  K. Sugioka,et al.  Micropatterning of neurons using organic substrates in culture , 1997 .

[2]  Mieko Matsuzawa,et al.  Chemically modifying glass surfaces to study substratum-guided neurite outgrowth in culture , 1996, Journal of Neuroscience Methods.

[3]  S. Thanos,et al.  A study in developing visual systems with a new method of staining neurones and their processes in fixed tissue. , 1987, Development.

[4]  K. Gotō,et al.  Cell-attachment activities of surface immobilized oligopeptides RGD, RGDS, RGDV, RGDT, and YIGSR toward five cell lines. , 1993, Journal of biomaterials science. Polymer edition.

[5]  G. Laurie,et al.  Biological activities of laminin , 1985, Journal of cellular biochemistry.

[6]  K. Ogomori,et al.  A synthetic peptide deduced from the sequence in the cross-region of laminin A chain mediates neurite outgrowth, cell attachment and heparin binding. , 1994, The Biochemical journal.

[7]  K. Sugioka,et al.  A Biocompatible Interface for the Geometrical Guidance of Central Neurons in Vitro , 1998 .

[8]  Yu-Chong Tai,et al.  Silicon cultured-neuron prosthetic devices for in vivo and in vitro studies , 1997 .

[9]  A. Vaheri,et al.  Neurons cultured from developing rat brain attach and spread preferentially to laminin , 1984, Journal of neuroscience research.

[10]  Y. Tai,et al.  The neurochip: a new multielectrode device for stimulating and recording from cultured neurons , 1999, Journal of Neuroscience Methods.

[11]  H. Kleinman,et al.  Identification of a second active site in laminin for promotion of cell adhesion and migration and inhibition of in vivo melanoma lung colonization. , 1989, Archives of biochemistry and biophysics.

[12]  A. Utani,et al.  Identification of Cell Binding Sites in the Laminin α1 Chain Carboxyl-terminal Globular Domain by Systematic Screening of Synthetic Peptides (*) , 1995, The Journal of Biological Chemistry.

[13]  A. Mercurio,et al.  Integrin signaling in leukocytes: lessons from the α6β1 integrin , 1997, Journal of leukocyte biology.

[14]  P. Clark.  Cell behaviour on micropatterned surfaces , 1994 .

[15]  G. McKhann,et al.  Selective growth of rat Schwann cells in neuron- and serum-free primary culture , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[16]  G J Brewer,et al.  Microcontact printing for precise control of nerve cell growth in culture. , 1999, Journal of biomechanical engineering.

[17]  P C Letourneau,et al.  Neurite extension by peripheral and central nervous system neurons in response to substratum-bound fibronectin and laminin. , 1983, Developmental biology.

[18]  D. Gullberg,et al.  Extracellular matrix and its receptors during development. , 1995, The International journal of developmental biology.

[19]  S. Thanos,et al.  Survival and Axonal Elongation of Adult Rat Retinal Ganglion Cells , 1989, The European journal of neuroscience.

[20]  A. Skubitz,et al.  A synthetic peptide derived from the carboxy terminus of the laminin A chain represents a binding site for the alpha 3 beta 1 integrin , 1992, The Journal of cell biology.

[21]  G. R. Martin,et al.  The RGD containing site of the mouse laminin A chain is active for cell attachment, spreading, migration and neurite outgrowth , 1991, Journal of cellular physiology.

[22]  D. Nicolau,et al.  Patterning neuronal and glia cells on light-assisted functionalised photoresists. , 1999, Biosensors & bioelectronics.

[23]  Friedrich Bonhoeffer,et al.  Position-dependent properties of retinal axons and their growth cones , 1985, Nature.

[24]  P. Aebischer,et al.  Neuronal cell attachment to fluorinated ethylene propylene films with covalently immobilized laminin oligopeptides YIGSR and IKVAV. II. , 1995, Journal of biomedical materials research.

[25]  J. Hubbell,et al.  Polymer networks with grafted cell adhesion peptides for highly biospecific cell adhesive substrates. , 1994, Analytical biochemistry.

[26]  A. Maelicke,et al.  Neuronal networks in vitro: formation and organization on biofunctionalized surfaces , 1999, Journal of materials science. Materials in medicine.

[27]  Andreas Offenhäusser,et al.  Electrophysiological development of embryonic hippocampal neurons from the rat grown on synthetic thin films , 1997, Neuroscience Letters.

[28]  H. Kleinman,et al.  Laminin A chain synthetic peptide which supports neurite outgrowth. , 1989, Biochemical and biophysical research communications.

[29]  R. Timpl,et al.  Basement membrane proteins: molecular structure and function. , 1988, Advances in protein chemistry.

[30]  D E Ingber,et al.  Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[31]  R. Murray,et al.  Optical, electrical, and electrochemical characteristics of ultrathin poly(phenylene oxide) films: organic dielectrics less than 10 nm thick , 1990 .

[32]  H. Kleinman,et al.  A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth. , 1989, The Journal of biological chemistry.

[33]  Sergio Martinoia,et al.  A simple microfluidic system for patterning populations of neurons on silicon micromachined substrates , 1999, Journal of Neuroscience Methods.

[34]  W. Göpel,et al.  Surface Attachment of Functional Peptides by Electrochemical Polymerisation , 1996 .

[35]  V. Castronovo,et al.  Laminin receptors and laminin-binding proteins during tumor invasion and metastasis. , 1993, Invasion & metastasis.

[36]  J. Hubbell,et al.  Covalent surface immobilization of Arg-Gly-Asp- and Tyr-Ile-Gly-Ser-Arg-containing peptides to obtain well-defined cell-adhesive substrates. , 1990, Analytical biochemistry.

[37]  Yukihide Iwamoto,et al.  Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding , 1987, Cell.

[38]  A. Mercurio.  Laminin receptors: achieving specificity through cooperation. , 1995, Trends in cell biology.

[39]  D. Castner,et al.  Surface Modification of Polymeric Biomaterials , 1997 .

[40]  S. Thanos,et al.  Modification of glassy carbon surfaces with synthetic laminin-derived peptides for nerve cell attachment and neurite growth. , 1998, Journal of biomedical materials research.

[41]  W. Göpel,et al.  Microstructured Peptide‐Functionalised Surfaces by Electrochemical Polymerisation , 1996 .

[42]  J Engel,et al.  Structure and function of laminin: anatomy of a multidomain glycoprotein , 1990, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[43]  M. Kano,et al.  Formation of hippocampal synapses on patterned substrates of a laminin‐derived synthetic peptide , 2000, The European journal of neuroscience.

[44]  R. Timpl,et al.  Laminin and other basement membrane components. , 1987, Annual review of cell biology.

[45]  J. Hubbell,et al.  Human endothelial cell interactions with surface-coupled adhesion peptides on a nonadhesive glass substrate and two polymeric biomaterials. , 1991, Journal of biomedical materials research.

[46]  G. Snounou,et al.  Identification of a neurite outgrowth‐promoting domain of laminin using synthetic peptides , 1989, FEBS letters.

[47]  A. Skubitz,et al.  A novel synthetic peptide from the B1 chain of laminin with heparin- binding and cell adhesion-promoting activities , 1988, The Journal of cell biology.