Embryonic development and growth performances of an endangered fish species Nandus nandus: effects of dietary polyunsaturated fatty acids supplementation

The present study explored the embryonic and larval development of an endangered fish species Nandus nandus and resolved larval growth performances with the dietary supplementation of different types of lipids. Fertilized eggs were collected from fiber glass tanks immediately after spontaneous spawning of N. nandus, which were fed with a 1% phospholipid (squid meal) supplemented diet for 3 months. Fertilized eggs were transparent, spherical, yellowish and sticky in nature. The first cleavages of eggs were observed 0.3±0.01 h post fertilization at 26 o C water temperature. Hatching started around 18 h post - fertilization and newly hatched larvae were found to be 1.2±0.1 mm in length. First feeding started 64.0±0.30 h post hatching. After rearing for 10 days, they were divided into 4 groups and separately fed with only dry tubificid worms, 1% docosahexaenoic (DHA) supplemented with dry tubificid worm, 1% phospholipid supplemented with dry tubificid worm and live tubificid worms as treatment I, treatment II, treatment III and treatment IV, respectively. After 50 days of the trial, larvae of treatment II showed significantly (p<0.01) higher growth performances in length: 3.18±0.13 cm, weight: 339.8 ± 36.94 mg and survival rate: 78±2 % which were comparable to that of treatment IV, which showed the highest (p<0.01) length of 3.4±0.1 cm, weight of 406.6±27.99 mg and survival rate of 97±1 %. Larvae in treatment I showed the lowest growth performances in length: 2.73 ± 0.16 cm, weight: 259.8±29.97 mg and survival rate of 63±3 %. As this is the first record for the determination of embryonic and larval development of N. nandus with different lipid supplemented diets, it might be possible to save this endangered fish species by adopting this technology at field level.

[1]  Z. Hossain,et al.  Performance of Different Dietary Lipids on Growth Indices and Survival of Striped Gourami, Colisa fasciatus (Perciformes: Osphronemidae) Fry , 2014 .

[2]  A. Roy,et al.  Growth Performance of Larvae Produced from Vitamin E Treated Female Clarias batrachus (Linnaeus) , 2013 .

[3]  Z. Hossain,et al.  Enhancement of reproductive performances of Gangetic leaffish, Nandus nandus through up regulation of serum Ca2+ concentration, improved morphological alteration of liver and ovary with dietary polyunsaturated fatty acids , 2013, Fish Physiology and Biochemistry.

[4]  C. Ngugi,et al.  The Effect of Three Different Feed Types on Growth Performance and Survival of African Catfish Fry (Clarias gariepinus) Reared in a Hatchery , 2012 .

[5]  C. Saad,et al.  Induced Breeding and Embryonic Development of Climbing Perch (Anabas testudineus, Bloch) , 2012 .

[6]  B. Dhar,et al.  Study of Bioenergetics, Proximate composition and Microbiological status of leaf fish Nandus nandus (Ham.1822) , 2012 .

[7]  K. Olurin,et al.  Larval rearing of African catfish, Clariasgariepinus fed decapsulated Artemia, wildcopepods or commercial starter diet , 2012 .

[8]  Yahya Bakhtiyar,et al.  Growth, survival and proximate body composition of Labeo rohita larvae fed artificial food and natural food organisms under laboratory condition , 2011 .

[9]  S. Steenfeldt,et al.  The effects of dietary long-chain essential fatty acids on growth and stress tolerance in pikeperch larvae (Sander lucioperca L.) , 2011 .

[10]  M. Rahman,et al.  Embryonic and larval development of guchibaim , Mastacembelus pancalus (Hamilton) , 2010 .

[11]  M. Gholami.  Effects of n-3 HUFA enriched Daphnia magna on growth, survival, stress resistance and fatty acid composition of white fish fry (Rutilus frisii kutum). , 2010 .

[12]  K. Olurin,et al.  Growth and Survival of African Catfish (Clarias gariepinus) Larvae Fed Decapsulated Artemia, Live Daphnia, or Commercial Starter Diet , 2010 .

[13]  R. Karuppasamy,et al.  Early embryonic and larval development of Indian catfish, Heteropneustes fossilis , 2009 .

[14]  S. Sadiku,et al.  Comparative Study of Artemia and Liqui-Fry in the Rearing of Clarias gariepinus Fry , 2009 .

[15]  M. Izquierdo,et al.  Modulation of ACTH-induced cortisol release by polyunsaturated fatty acids in interrenal cells from gilthead seabream, Sparus aurata. , 2006, The Journal of endocrinology.

[16]  J. Navarro,et al.  Dietary deficiency of docosahexaenoic acid impairs vision at low light intensities in juvenile herring (Clupea harengus L.) , 1995, Lipids.

[17]  D. Tocher,et al.  Specific accumulation of docosahexaenoic acid (22∶6n−3) in brain lipids during development of juvenile turbotScophthalmus maximus L. , 1991, Lipids.

[18]  J. Dick,et al.  Molecular species composition of the major diacyl glycerophospholipids from muscle, liver, retina and brain of cod (Gadus morhua) , 1991, Lipids.

[19]  A. Islam.  Embryonic and larval development of Thai Pangas (Pangasius sutchi Fowler, 1937) , 2005, Development, growth & differentiation.

[20]  A. Skalli,et al.  Requirement of n-3 long chain polyunsaturated fatty acids for European sea bass (Dicentrarchus labrax) juveniles: growth and fatty acid composition , 2004 .

[21]  Yoshimi Sato,et al.  Embriogenesis and larval ontogeny of the "piau-gordura", Leporinus piau (Fowler) (Pisces, Anostomidae) after induced spawning , 2004 .

[22]  M. T. Dinis,et al.  Digestive enzymes profile of Solea senegalensis post larvae fed Artemia and a compound diet , 2002, Fish Physiology and Biochemistry.

[23]  N. Roos,et al.  14 SMALL INDIGENOUS FISH SPECIES IN AQUACULTURE IN BANGLADESH : CONTRIBUTION TO VITAMIN A , CALCIUM , AND IRON INTAKES , 2004 .

[24]  .. H.Rashid,et al.  First Record of Artificial Spawning of Nandus nandus (Hamiltom) in Bangladesh Using Carp Pituitary Gland: An Endangered Species Bred in Captivity , 2003 .

[25]  N. Roos,et al.  Small indigenous fish species in bangladesh: contribution to vitamin A, calcium and iron intakes. , 2003, The Journal of nutrition.

[26]  Kyoung‐Duck Kim,et al.  Effect of dietary essential fatty acids on growth, body composition and blood chemistry of juvenile starry flounder (Platichthys stellatus) , 2003 .

[27]  D. Tocher.  Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish , 2003 .

[28]  M. Nagarajan,et al.  Embryonic and larval development of spotted murrel, Channa punctatus (Bloch) , 2003 .

[29]  C. Parrish,et al.  Effects of docosahexaenoic, eicosapentaenoic, and arachidonic acids on the early growth, survival, lipid composition and pigmentation of yellowtail flounder (Limanda ferruginea): a live food enrichment experiment , 2002 .

[30]  M. Kentouri,et al.  Osteological development of Dentex dentex (Osteichthyes: Sparidae): dorsal, anal, paired fins and squamation , 2001 .

[31]  T. Takeuchi,et al.  Effect of different levels of eicosapentaenoic acid and docosahexaenoic acid in Artemia nauplii on growth, survival and salinity tolerance of larvae of the Japanese flounder, Paralichthys olivaceus , 1999 .

[32]  I. Geurden,et al.  Histological changes induced by dietary phospholipids in intestine and liver of common carp (Cyprinus carpio L.) larvae , 1998 .

[33]  R. J. Henderson,et al.  The biosynthesis of docosahexaenoic acid [22:6(n-3)] from linolenic acid in primary hepatocytes isolated from wild northern pike. , 1997, Journal of fish biology.

[34]  T. Takeuchi.  Essential fatty acid requirements of aquatic animals with emphasis on fish larvae and fingerlings , 1997 .

[35]  J. Navarro,et al.  Deficit of didocosahexaenoyl phospholipid in the eyes of larval sea bass fed an essential fatty acid deficient diet , 1996 .

[36]  R. Henderson.  Fatty acid metabolism in freshwater fish with particular reference to polyunsaturated fatty acids. , 1996, Archiv fur Tierernahrung.

[37]  J. Navarro,et al.  Behavioural differences in starving herring Clupea harengus L. larvae correlate with body levels of essential fatty acids , 1992 .

[38]  Koretarō Takahashi,et al.  Application of Partition Chromatographic Theory for the Analysis of Marine Triglyceride Molecular Species , 1988 .

[39]  Af Prevot,et al.  Utilisation des colonnes capillaires de verre pour l’analyse des corps gras par chromatographie en phase gazeuse. , 1976 .

[40]  A. L. Bhuiyan.  Fishes of Dacca , 1964 .