In: The Arabidopsis Book

Fresh leaf tissue and seed were lysed and extracted according to the P-PER Kit (Product # 89803) protocol, a competitor’s protocol and a literature-based (home brew) protocol. Samples were normalized (weight tissue/volume extract), resolved on a 10% Bis-Tris gel and stained with ImperialTM Protein Stain† (Product # 24615). Samples were also quantified using the BCATM Protein Assay Kit, Reducing Agent Compatible (Product # 23250).