Characterization of lipoprotein IRP1 from Corynebacterium diphtheriae, which is regulated by the diphtheria toxin repressor (DtxR) and iron

The Corynebacterium diphtheriae irp1 gene is negatively regulated by DtxR and iron. The nucleotide sequence of irp1 revealed that it has homology with genes involved in iron acquisition. Expression of the irp1 gene showed that it encodes a lipoprotein (IRP1) with a predicted size of 38 kDa. Northern blot experiments indicated that transcription from the irp1 promoter is repressed in high-iron medium and suggested that irp1 is part of an iron-regulated operon.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  M. Schmitt,et al.  Identification and characterization of three new promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor (DtxR) and iron , 1997, Infection and immunity.

[3]  M. Schmitt.  Utilization of host iron sources by Corynebacterium diphtheriae: identification of a gene whose product is homologous to eukaryotic heme oxygenases and is required for acquisition of iron from heme and hemoglobin , 1997, Journal of bacteriology.

[4]  A. Neely,et al.  Pyoverdin is essential for virulence of Pseudomonas aeruginosa , 1996, Infection and immunity.

[5]  D. Ringe,et al.  Iron, DtxR, and the regulation of diphtheria toxin expression , 1994, Molecular microbiology.

[6]  F. Cabello,et al.  Salmonella typhi iron uptake mutants are attenuated in mice , 1994, Infection and immunity.

[7]  T. A. Krulwich,et al.  Isolation of Tn917 insertional mutants of Bacillus subtilis that are resistant to the protonophore carbonyl cyanide m-chlorophenylhydrazone. , 1994, Biochimica et biophysica acta.

[8]  M. Schmitt,et al.  Cloning, sequence, and footprint analysis of two promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor (DtxR) and iron , 1994, Journal of bacteriology.

[9]  M. Schmitt,et al.  Analysis of diphtheria toxin repressor‐operator interactions and characterization of a mutant repressor with decreased binding activity for divalent metals , 1993, Molecular microbiology.

[10]  K. Hantke,et al.  Iron‐hydroxamate uptake systems in Bacillus subtilis: identification of a lipoprotein as part of a binding protein‐dependent transport system , 1993, Molecular microbiology.

[11]  S. Calderwood,et al.  Role of iron in regulation of virulence genes , 1993, Clinical Microbiology Reviews.

[12]  M. Schmitt,et al.  Purification and characterization of the diphtheria toxin repressor. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[13]  M. Schmitt,et al.  Characterization of a defective diphtheria toxin repressor (dtxR) allele and analysis of dtxR transcription in wild-type and mutant strains of Corynebacterium diphtheriae , 1991, Infection and immunity.

[14]  M. Schmitt,et al.  Iron-dependent regulation of diphtheria toxin and siderophore expression by the cloned Corynebacterium diphtheriae repressor gene dtxR in C. diphtheriae C7 strains , 1991, Infection and immunity.

[15]  R. F. Wang,et al.  Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. , 1991, Gene.

[16]  R. Holmes,et al.  Coordinate regulation of siderophore and diphtheria toxin production by iron in Corynebacterium diphtheriae. , 1990, Microbial pathogenesis.

[17]  J. Murphy,et al.  Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[18]  J. Murphy,et al.  Characterization of the diphtheria tox transcript in Corynebacterium diphtheriae and Escherichia coli , 1985, Journal of bacteriology.

[19]  R. Holmes,et al.  Highly toxinogenic but avirulent Park-Williams 8 strain of Corynebacterium diphtheriae does not produce siderophore , 1985, Infection and immunity.

[20]  C. Richardson,et al.  A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[21]  R. Holmes,et al.  Genetic and biochemical evidence for a siderophore-dependent iron transport system in Corynebacterium diphtheriae , 1984, Infection and immunity.

[22]  R. Holmes,et al.  Regulation of toxinogenesis in Corynebacterium diphtheriae: mutations in the bacterial genome that alter the effects of iron on toxin production , 1983, Journal of bacteriology.

[23]  N. Groman,et al.  Plasmids in Corynebacterium diphtheriae and diphtheroids mediating erythromycin resistance , 1980, Antimicrobial Agents and Chemotherapy.

[24]  P. Williams.  Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli , 1979, Infection and immunity.

[25]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[26]  R. Holmes,et al.  Genetic Analysis of tox+ and tox− Bacteriophages of Corynebacterium diphtheriae , 1969, Journal of virology.

[27]  R. Holmes,et al.  Genetic Analysis of tox+ and tox− Bacteriophages of Corynebacterium diphtheriae , 1969 .

[28]  A. M. Pappenheimer.  Diphtheria toxin. , 1952, Annual review of biochemistry.