Hydrophilic elastomeric biomaterials based on resilin-like polypeptides.

The production of complex, yet well defined materials offers many opportunities in regenerative medicine, in which the mechanical and biological properties of the matrix must meet stringent requirements. Here we report the recombinant production of modular polypeptidic materials, based on the highly resilient protein resilin, which are equipped with multiple biologically active domains. The recombinant materials exhibit useful mechanical and cell adhesion behavior.

[1]  T. M. Parker,et al.  Temperature of polypeptide inverse temperature transition depends on mean residue hydrophobicity , 1991 .

[2]  T. Weis-Fogh.  Molecular interpretation of the elasticity of resilin, a rubber-like protein , 1961 .

[3]  Henry H. Mantsch,et al.  Infrared spectroscopy of biomolecules , 1996 .

[4]  Ashutosh Chilkoti,et al.  In situ cross-linking of elastin-like polypeptide block copolymers for tissue repair. , 2008, Biomacromolecules.

[5]  J. M. Harris,et al.  Poly(Ethylene Glycol) Chemistry Biotechnical and Biomedical Applications , 1992 .

[6]  T. Weis-Fogh.  A Rubber-Like Protein in Insect Cuticle , 1960 .

[7]  Horst Kessler,et al.  RGD modified polymers: biomaterials for stimulated cell adhesion and beyond. , 2003, Biomaterials.

[8]  A. Chilkoti,et al.  Biomedical and Biotechnological Applications of Elastin-Like Polypeptides , 2007 .

[9]  Xiao Huang,et al.  Biodegradable thermoresponsive hydrogels for aqueous encapsulation and controlled release of hydrophilic model drugs. , 2005, Biomacromolecules.

[10]  T. Vuocolo,et al.  Synthesis and properties of crosslinked recombinant pro-resilin , 2005, Nature.

[11]  Ashutosh Chilkoti,et al.  Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair. , 2005, Tissue engineering.

[12]  Christian Franck,et al.  Mechanically Tunable Thin Films of Photosensitive Artificial Proteins: Preparation and Characterization by Nanoindentation , 2008 .

[13]  J. Hubbell,et al.  Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering , 2005, Nature Biotechnology.

[14]  G. Fields,et al.  Human matrix metalloproteinase specificity studies using collagen sequence-based synthetic peptides. , 1996, Biopolymers.

[15]  E. Blout,et al.  Conformational investigations on the polypeptide and oligopeptides with the repeating sequence L-alanyl-L-prolylglycine. , 1971, Biochemistry.

[16]  Ashutosh Chilkoti,et al.  Evaluation of an elastin-like polypeptide-doxorubicin conjugate for cancer therapy. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[17]  R. Lyons,et al.  High yield expression of recombinant pro-resilin: lactose-induced fermentation in E. coli and facile purification. , 2007, Protein expression and purification.

[18]  Liang Feng,et al.  Simple bioseparations using self-cleaving elastin-like polypeptide tags , 2005, Nature Methods.

[19]  Skals,et al.  Sound production by abdominal tymbal organs in two moth species: the green silver-line and the scarce silver-line (Noctuoidea: Nolidae: Chloephorinae). , 1999, The Journal of experimental biology.

[20]  T. M. Parker,et al.  Hydrophobicity scale for proteins based on inverse temperature transitions , 1992, Biopolymers.

[21]  Sarah C. Heilshorn,et al.  Independent tuning of multiple biomaterial properties using protein engineering , 2009 .

[22]  C. Chu,et al.  Biodegradable dextran-polylactide hydrogel network and its controlled release of albumin. , 2001, Journal of biomedical materials research.

[23]  J. Vincent,et al.  Design and mechanical properties of insect cuticle. , 2004, Arthropod structure & development.

[24]  T. Yamaoka,et al.  Design and Biosynthesis of Elastin-like Artificial Extracellular Matrix Proteins Containing Periodically Spaced Fibronectin CS5 Domains , 1999 .

[25]  P. Flory.  Principles of polymer chemistry , 1953 .

[26]  D. Tirrell,et al.  Engineering the extracellular matrix: a novel approach to polymeric biomaterials. I. Control of the physical properties of artificial protein matrices designed to support adhesion of vascular endothelial cells. , 2000, Biomacromolecules.

[27]  L. Setton,et al.  Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution. , 2007, Biomacromolecules.

[28]  G. Robillard,et al.  Structure characterization of the central repetitive domain of high molecular weight gluten proteins. I. Model studies using cyclic and linear peptides , 1997, Protein science : a publication of the Protein Society.

[29]  P. Janmey,et al.  Tissue Cells Feel and Respond to the Stiffness of Their Substrate , 2005, Science.

[30]  D. Carson,et al.  A heparin-binding synthetic peptide of heparin/heparan sulfate-interacting protein modulates blood coagulation activities. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[31]  V. Conticello,et al.  Self-assembly of block copolymers derived from elastin-mimetic polypeptide sequences. , 2002, Advanced drug delivery reviews.

[32]  A. Fertala,et al.  Testing the utility of rationally engineered recombinant collagen-like proteins for applications in tissue engineering. , 2006, Journal of biomedical materials research. Part A.

[33]  David A. Tirrell,et al.  Mechanical Properties of Artificial Protein Matrices Engineered for Control of Cell and Tissue Behavior , 2003 .

[34]  David A Tirrell,et al.  Cell-binding domain context affects cell behavior on engineered proteins. , 2005, Biomacromolecules.

[35]  Brigida Bochicchio,et al.  Investigating by CD the molecular mechanism of elasticity of elastomeric proteins. , 2008, Chirality.

[36]  Alyssa Panitch,et al.  Biologically engineered protein-graft-poly(ethylene glycol) hydrogels: a cell adhesive and plasmin-degradable biosynthetic material for tissue repair. , 2002, Biomacromolecules.