Plasmonic Sensing of Biological Analytes Through Nanoholes

A transmission-based surface plasmon resonance (SPR) sensor for label-free detection of protein-carbohydrate and protein-protein binding proximate to a perforated gold surface is demonstrated. An SPR instrument makes real-time measurements of the resonant wavelength and/or the resonant angle of incidence of transmitted light; both are influenced by the presence of proteins at the gold surface-liquid interface. Ethylene glycol solutions with known refractive indexes were used to calibrate the instrument. A paired polarization-sensitive detector achieved an overall detection resolution of ~ 6.6 times 10-5 refractive index units (RIU). Proof of principle experiments was performed with concanavalin A (Con A) binding to gold-adsorbed ovomucoid and anti-bovine serum albumin (BSA) binding to gold-adsorbed BSA.

[1]  A. Mangili,et al.  Review Transmission , 2022 .

[2]  Y. Fainman,et al.  Excitation and direct imaging of surface plasmon polariton modes in a two-dimensional grating , 2005 .

[3]  G. Steiner,et al.  Surface plasmon resonance imaging , 2004, Analytical and bioanalytical chemistry.

[4]  D. Sinton,et al.  On-chip surface-based detection with nanohole arrays. , 2007, Analytical chemistry.

[5]  Yeshaiahu Fainman,et al.  Spectral sensitivity of two-dimensional nanohole array surface plasmon polariton resonance sensor , 2007 .

[6]  J. Homola.  Present and future of surface plasmon resonance biosensors , 2003, Analytical and bioanalytical chemistry.

[7]  H. Ho,et al.  Surface Plasmon Resonance Biosensor Incorporated in a Michelson Interferometer WithEnhanced Sensitivity , 2007, IEEE Sensors Journal.

[8]  Yeshaiahu Fainman,et al.  Observation of the splitting of degenerate surface plasmon polariton modes in a two-dimensional metallic nanohole array , 2007 .

[9]  Joshua LaBaer,et al.  Emerging tools for real‐time label‐free detection of interactions on functional protein microarrays , 2005, The FEBS journal.

[10]  D. Myszka,et al.  Survey of the year 2005 commercial optical biosensor literature , 2006, Journal of molecular recognition : JMR.

[11]  T. Ebbesen,et al.  Light in tiny holes , 2007, Nature.

[12]  Rajan P Kulkarni,et al.  Label-Free, Single-Molecule Detection with Optical Microcavities , 2007, Science.

[13]  Nicolle H. Packer,et al.  GlycoSuiteDB: a new curated relational database of glycoprotein glycan structures and their biological sources , 2001, Nucleic Acids Res..

[14]  Peter Kauffman,et al.  Portable 24-analyte surface plasmon resonance instruments for rapid, versatile biodetection. , 2007, Biosensors & bioelectronics.

[15]  J. Justin Gooding,et al.  Biosensor technology for detecting biological warfare agents : Recent progress and future trends , 2006 .

[16]  Yoshihiro Kawaoka,et al.  Avian flu: Influenza virus receptors in the human airway , 2006, Nature.

[17]  H. Raether.  Surface Plasmons on Smooth and Rough Surfaces and on Gratings , 1988 .

[18]  Peter Kauffman,et al.  Airborne analyte detection with an aircraft-adapted surface plasmon resonance sensor system , 2005 .

[19]  Nicholas O Fischer,et al.  Heightened sense for sensing: recent advances in pathogen immunoassay sensing platforms. , 2007, The Analyst.

[20]  Paul Yager,et al.  Compact, high performance surface plasmon resonance imaging system. , 2007, Biosensors & bioelectronics.

[21]  R. Rich,et al.  Survey of the year 2004 commercial optical biosensor literature , 2005, Journal of molecular recognition : JMR.

[22]  A. Srinivasan,et al.  Glycan topology determines human adaptation of avian H5N1 virus hemagglutinin , 2008, Nature Biotechnology.

[23]  Y. Fainman,et al.  High-resolution surface plasmon resonance sensor based on linewidth-optimized nanohole array transmittance. , 2006, Optics letters.

[24]  Ramaier Narayanaswamy,et al.  Analysis of the performance of interferometry, surface plasmon resonance and luminescence as biosensors and chemosensors , 2006 .

[25]  Hiren J. Joshi,et al.  GlycoSuiteDB: a curated relational database of glycoprotein glycan structures and their biological sources. 2003, update , 2003, Nucleic Acids Res..

[26]  Thomas W. Ebbesen,et al.  Surface plasmons enhance optical transmission through subwavelength holes , 1998 .

[27]  H. Lezec,et al.  Extraordinary optical transmission through sub-wavelength hole arrays , 1998, Nature.

[28]  Fredrik Höök,et al.  Improving the instrumental resolution of sensors based on localized surface plasmon resonance. , 2006, Analytical chemistry.

[29]  J. Rogers,et al.  Quantitative multispectral biosensing and 1D imaging using quasi-3D plasmonic crystals , 2006, Proceedings of the National Academy of Sciences.

[30]  K. Kavanagh,et al.  Surface plasmon sensor based on the enhanced light transmission through arrays of nanoholes in gold films. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[31]  Quan Cheng,et al.  Recent advances in surface plasmon resonance based techniques for bioanalysis , 2007, Analytical and bioanalytical chemistry.