Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin–AcGFP in Z discs

Nanoscale imaging of cultured cardiomyocytes allows the quantitative assessment of changes in the length of single sarcomeres during contractile events.

[1]  E. A. Lima,et al.  Differential effects of phospholamban and Ca2+/calmodulin-dependent kinase II on [Ca2+]i transients in cardiac myocytes at physiological stimulation frequencies. , 2008, American journal of physiology. Heart and circulatory physiology.

[2]  Peter Kohl,et al.  Fast Measurement of Sarcomere Length and Cell Orientation in Langendorff-Perfused Hearts Using Remote Focusing Microscopy , 2013, Circulation research.

[3]  W. Welch,et al.  The pharmacology of ryanodine and related compounds. , 1997, Pharmacological reviews.

[4]  Shin'ichi Ishiwata,et al.  Mechanochemical coupling of two substeps in a single myosin V motor , 2004, Nature Structural &Molecular Biology.

[5]  Toshio Yanagida,et al.  Four-dimensional spatial nanometry of single particles in living cells using polarized quantum rods. , 2013, Biophysical journal.

[6]  T. Pozzan,et al.  Mitochondrial Ca2+ uptake contributes to buffering cytoplasmic Ca2+ peaks in cardiomyocytes , 2012, Proceedings of the National Academy of Sciences.

[7]  S. Ishiwata,et al.  Length Dependence of Tension Generation in Rat Skinned Cardiac Muscle: Role of Titin in the Frank-Starling Mechanism of the Heart , 2001, Circulation.

[8]  Michael Regnier,et al.  Substrate stiffness increases twitch power of neonatal cardiomyocytes in correlation with changes in myofibril structure and intracellular calcium. , 2011, Biophysical journal.

[9]  S. Ishiwata,et al.  A theory on auto-oscillation and contraction in striated muscle. , 2011, Progress in biophysics and molecular biology.

[10]  T. Pozzan,et al.  Ca2+ oscillation frequency decoding in cardiac cell hypertrophy: Role of calcineurin/NFAT as Ca2+ signal integrators , 2008, Proceedings of the National Academy of Sciences.

[11]  D. Cox,et al.  Cardiac Myosin Activation: A Potential Therapeutic Approach for Systolic Heart Failure , 2011, Science.

[12]  S. Ishiwata,et al.  Contractile system of muscle as an auto-oscillator. , 2011, Progress in biophysics and molecular biology.

[13]  W. P. Smotherman,et al.  Heart rate response of the rat fetus and neonate to a chemosensory stimulus , 1991, Physiology & Behavior.

[14]  Y. Kuramoto,et al.  Locally and globally coupled oscillators in muscle. , 2013, Physical review letters.

[15]  Andrew D McCulloch,et al.  Substrate stiffness affects the functional maturation of neonatal rat ventricular myocytes. , 2008, Biophysical journal.

[16]  Noriaki Ohuchi,et al.  In Vivo Nano-imaging of Membrane Dynamics in Metastatic Tumor Cells Using Quantum Dots* , 2009, The Journal of Biological Chemistry.

[17]  D. Bers Cardiac excitation–contraction coupling , 2002, Nature.

[18]  R. Varhol,et al.  Kinetics of Thapsigargin- Ca-ATPase (Sarcoplasmic Reticulum) Interaction Reveals a Two-step Binding Mechanism and Picomolar Inhibition (*) , 1995, The Journal of Biological Chemistry.

[19]  D. Allen,et al.  The cellular basis of the length-tension relation in cardiac muscle. , 1985, Journal of molecular and cellular cardiology.

[20]  S. Christensen,et al.  A tool coming of age: thapsigargin as an inhibitor of sarco-endoplasmic reticulum Ca(2+)-ATPases. , 1998, Trends in pharmacological sciences.

[21]  J. Sanger,et al.  Myofibrillogenesis visualized in living embryonic cardiomyocytes. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[22]  S. Ishiwata,et al.  Myocardial sarcomeres spontaneously oscillate with the period of heartbeat under physiological conditions. , 2006, Biochemical and biophysical research communications.

[23]  S. Ishiwata,et al.  Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients. , 2012, Biochemical and biophysical research communications.

[24]  G. Dreissen,et al.  The constant beat: cardiomyocytes adapt their forces by equal contraction upon environmental stiffening , 2013, Biology Open.

[25]  I. Ohtsuki,et al.  Depressed Frank-Starling mechanism in the left ventricular muscle of the knock-in mouse model of dilated cardiomyopathy with troponin T deletion mutation ΔK210. , 2013, Journal of molecular and cellular cardiology.

[26]  Kerry S McDonald,et al.  Cardiac function and modulation of sarcomeric function by length. , 2008, Cardiovascular research.

[27]  William A Mohler,et al.  Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres. , 2006, Biophysical journal.

[28]  R. Moss,et al.  Understanding the Organisation and Role of Myosin Binding Protein C in Normal Striated Muscle by Comparison with MyBP-C Knockout Cardiac Muscle , 2008, Journal of molecular biology.

[29]  Yiming Wu,et al.  Developmental Control of Titin Isoform Expression and Passive Stiffness in Fetal and Neonatal Myocardium , 2004, Circulation research.

[30]  A. McCulloch,et al.  Cardiac myocyte force development during differentiation and maturation , 2010, Annals of the New York Academy of Sciences.

[31]  Marko Vendelin,et al.  Real-time determination of sarcomere length of a single cardiomyocyte during contraction , 2012, American journal of physiology. Cell physiology.

[32]  C. Gregorio,et al.  Muscle assembly: a titanic achievement? , 1999, Current opinion in cell biology.

[33]  Thierry Boulesteix,et al.  Second-harmonic microscopy of unstained living cardiac myocytes: measurements of sarcomere length with 20-nm accuracy. , 2004, Optics letters.

[34]  Hiroyuki Moriguchi,et al.  Two-dimensional network formation of cardiac myocytes in agar microculture chip with 1480 nm infrared laser photo-thermal etching. , 2003, Lab on a chip.

[35]  Shin'ichi Ishiwata,et al.  Real-time measurement of the length of a single sarcomere in rat ventricular myocytes: a novel analysis with quantum dots. , 2011, American journal of physiology. Cell physiology.

[36]  Scott L. Delp,et al.  Minimally invasive high-speed imaging of sarcomere contractile dynamics in mice and humans , 2008, Nature.

[37]  Noriaki Ohuchi,et al.  In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice. , 2007, Cancer research.

[38]  C Bollensdorff,et al.  Measurement and analysis of sarcomere length in rat cardiomyocytes in situ and in vitro. , 2010, American journal of physiology. Heart and circulatory physiology.

[39]  H. Higuchi,et al.  Intracellular imaging of targeted proteins labeled with quantum dots. , 2008, Experimental cell research.

[40]  A. Katz,et al.  Ernest Henry Starling, His Predecessors, and the “Law of the Heart” , 2002, Circulation.

[41]  Vladimir Benes,et al.  Developmentally Regulated Switching of Titin Size Alters Myofibrillar Stiffness in the Perinatal Heart , 2004, Circulation research.

[42]  M. Kaya,et al.  Nonlinear Elasticity and an 8-nm Working Stroke of Single Myosin Molecules in Myofilaments , 2010, Science.

[43]  S. Ishiwata,et al.  Disuse-induced Preferential Loss of the Giant Protein Titin Depresses Muscle Performance via Abnormal Sarcomeric Organization , 2008, The Journal of general physiology.

[44]  H. Granzier,et al.  Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells , 2011, The Journal of general physiology.

[45]  B. Iorga,et al.  Mechanical properties of sarcomeres during cardiac myofibrillar relaxation: stretch-induced cross-bridge detachment contributes to early diastolic filling , 2006, Journal of Muscle Research & Cell Motility.

[46]  T. Yanagida,et al.  Simultaneous measurement of nucleotide occupancy and mechanical displacement in myosin-V, a processive molecular motor. , 2009, Biophysical journal.

[47]  A. Morgan,et al.  Ionomycin enhances Ca2+ influx by stimulating store-regulated cation entry and not by a direct action at the plasma membrane. , 1994, The Biochemical journal.

[48]  R. W. Taylor,et al.  Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria. , 1980, The Journal of biological chemistry.

[49]  L. Wilkins Late-Breaking Clinical Trial Abstracts , 2002 .