Hemagglutinin, urease, and hemolysin production by Proteus mirabilis from clinical sources.

Proteus mirabilis, a common cause of urinary tract infection, can lead to serious complications including pyelonephritis. Adherence factors, urease, and hemolysin may be virulence determinants. These factors were compared for bacteria cultured from 16 patients with acute pyelonephritis and 35 with catheter-associated bacteriuria and for 20 fecal isolates. Pyelonephritis isolates were more likely (P less than .05) to express the mannose-resistant/Proteus-like (MR/P) hemagglutinin in the absence of mannose-resistant/Klebsiella-like (MR/K) hemagglutinin than were catheter-associated or fecal isolates. Pyelonephritis isolates produced urease activity of 63 +/- 27 (mean +/- SD) mumol of NH3/min/mg of protein, not significantly different from catheter-associated or fecal isolates. Hybridization of Southern blots of P. mirabilis chromosomal DNA with two urease gene probes demonstrated that urease gene sequences were conserved in all isolates. Geometric mean of reciprocal hemolytic titers for pyelonephritis isolates was 27.9; for urinary catheter isolates, 18.0; and for fecal isolates, 55.7 (not significantly different, P greater than .1). Although in vivo expression of urease and hemolysin may not be reliable indexes of virulence, MR/P hemagglutination in the absence of MR/K hemagglutination may be necessary for development of pyelonephritis.

[1]  R. Hausinger,et al.  Microbial ureases: significance, regulation, and molecular characterization. , 1989, Microbiological reviews.

[2]  H. Mobley,et al.  Proteus mirabilis urease: genetic organization, regulation, and expression of structural genes , 1988, Journal of bacteriology.

[3]  J. Tenney,et al.  MR/K hemagglutination of Providencia stuartii correlates with adherence to catheters and with persistence in catheter-associated bacteriuria. , 1988, The Journal of infectious diseases.

[4]  C. Hughes,et al.  Production and properties of haemolysins from clinical isolates of the Proteeae. , 1988, Journal of medical microbiology.

[5]  H. Mobley,et al.  Urease-positive bacteriuria and obstruction of long-term urinary catheters , 1987, Journal of clinical microbiology.

[6]  R. Welch.  Identification of two different hemolysin determinants in uropathogenic Proteus isolates , 1987, Infection and immunity.

[7]  M. Kerr,et al.  Proteus mirabilis strains of diverse type have IgA protease activity. , 1987, Journal of medical microbiology.

[8]  H. Mobley,et al.  Genetic and biochemical diversity of ureases of Proteus, Providencia, and Morganella species isolated from urinary tract infection , 1987, Infection and immunity.

[9]  V. Koronakis,et al.  The secreted hemolysins of Proteus mirabilis, Proteus vulgaris, and Morganella morganii are genetically related to each other and to the alpha-hemolysin of Escherichia coli , 1987, Journal of Bacteriology.

[10]  R. Cook,et al.  Identification and characterization of a uroepithelial cell adhesin from a uropathogenic isolate of Proteus mirabilis , 1986, Infection and Immunity.

[11]  H. Mobley,et al.  Cloning of urease gene sequences from Providencia stuartii , 1986, Infection and immunity.

[12]  C. Bauer,et al.  The combination of pivampicillin and pivmecillinam versus pivampicillin alone in the treatment of acute pyelonephritis. , 1986, Scandinavian journal of infectious diseases.

[13]  T. File,et al.  Timentin versus piperacillin in the therapy of serious urinary tract infections. , 1985, The American journal of medicine.

[14]  S Falkow,et al.  Gal-Gal binding and hemolysin phenotypes and genotypes associated with uropathogenic Escherichia coli. , 1985, The New England journal of medicine.

[15]  R. Adegbola,et al.  Antigenic relationships among type-3 fimbriae of Enterobacteriaceae revealed by immunoelectronmicroscopy. , 1985, Journal of medical microbiology.

[16]  R. Gleckman,et al.  Therapy of symptomatic pyelonephritis in women. , 1985, The Journal of urology.

[17]  U. Setia,et al.  Bacteremia in a long-term care facility. Spectrum and mortality. , 1984, Archives of internal medicine.

[18]  D. MacLaren,et al.  Vero cell invasiveness of Proteus mirabilis , 1984, Infection and immunity.

[19]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[20]  R. Adegbola,et al.  The adhesins and fimbriae of Proteus mirabilis strains associated with high and low affinity for the urinary tract. , 1983, Journal of medical microbiology.

[21]  A. Feinberg,et al.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. , 1983, Analytical biochemistry.

[22]  B. Jacobsson,et al.  Correlation of P blood group, vesicoureteral reflux, and bacterial attachment in patients with recurrent pyelonephritis. , 1983, The New England journal of medicine.

[23]  W. Anthony,et al.  A prospective microbiologic study of bacteriuria in patients with chronic indwelling urethral catheters. , 1982, The Journal of infectious diseases.

[24]  R. Adegbola,et al.  Haemagglutinins and fimbriae of Morganella, Proteus and Providencia. , 1982, Journal of medical microbiology.

[25]  R. Gleckman,et al.  Symptomatic Pyelonephritis in Elderly Men , 1982, Journal of the American Geriatrics Society.

[26]  L. Gentry,et al.  Cefamandole alone and combined with gentamicin or tobramycin in the treatment of acute pyelonephritis. , 1980, Scandinavian Journal of Infectious Diseases. Supplementum.

[27]  J. Hamilton-miller,et al.  Rapid screening for urease inhibitors. , 1979, Investigative urology.

[28]  I. Ofek,et al.  Influence of pili on the virulence of Proteus mirabilis in experimental hematogenous pyelonephritis. , 1978, The Journal of infectious diseases.

[29]  T. Stamey,et al.  Studies of introital colonization in women with recurrent urinary infections. X. Adhesive properties of Escherichia coli and Proteus mirabilis: lack of correlation with urinary pathogenicity. , 1978, The Journal of urology.

[30]  F. Silverblatt.  HOST-PARASITE INTERACTION IN THE RAT RENAL PELVIS , 1974, The Journal of experimental medicine.

[31]  Pazin Gj,et al.  Immobilizing antibodies in urine. II. Prevention of ascending spread of Proteus mirabilis. , 1974 .

[32]  P. Gower,et al.  The site of infection in 133 patients with bacteriuria. , 1973, Clinical nephrology.

[33]  D. MacLaren,et al.  The significance of urease in proteus pyelonephritis: a bacteriological study. , 1968, The Journal of pathology and bacteriology.

[34]  K. Fairley,et al.  Simple test to determine the site of urinary-tract infection. , 1967, Lancet.

[35]  J. Marmur.  A procedure for the isolation of deoxyribonucleic acid from micro-organisms , 1961 .

[36]  A. Braude,et al.  ROLE OF BACTERIAL UREASE IN EXPERIMENTAL PYELONEPHRITIS , 1960, Journal of bacteriology.