Production of planktonic bacteria in Lake Michigan

Bacterial production rates were estimated for the surface waters of a station 100-m deep in southeastern Lake Michigan during 1984. Production was calculated from incorporation of C3Hmethyllthymidine and from empirical conversion factors determined from dilution experiments performed throughout the study. The conversion factors (with typical C.V. ~40%) varied between 4.7 and 18.3 x lo9 cells produced per nanomole of thymidine incorporated into ice-cold trichloroacetic acid extracts. Our estimates yielded bacteria exponential growth rates between 0.05 and 0.24 h-l (C.V. typically ~50%) based on the empirical conversion factors. The growth estimates are much lower (0.004-0.020 h-l) when based on measured 47% thymidine incorporation into DNA and a theoretical conversion factor. The higher growth estimates appear more consistent with estimated grazing losses. Carbon flux estimates are less certain, due to the possible range of bacterial carbon content and growth efficiencies, but most of the higher growth estimates imply a bacterial carbon demand higher than concurrent 14C-based primary production measurements. This may mean that a source other than recent primary production is needed to meet this demand.

[1]  M. Klug,et al.  Current Perspectives in Microbial Ecology , 1985 .

[2]  G. Bratbak,et al.  Bacterial Biovolume and Biomass Estimations , 1985, Applied and environmental microbiology.

[3]  F. Azam,et al.  Bacterial 5-Nucleotidase in Aquatic Ecosystems: A Novel Mechanism of Phosphorus Regeneration , 1985, Science.

[4]  J. C. Goldman,et al.  Susceptibility of some marine phytoplankton species to cell breakage during filtration and post-filtration rinsing , 1985 .

[5]  H. Ducklow,et al.  Tritiated thymidine incorporation and the growth of heterotrophic bacteria in warm core rings , 1985 .

[6]  A. Konopka,et al.  Primary and Bacterial Production in Two Dimictic Indiana Lakes , 1985, Applied and environmental microbiology.

[7]  D. Caron,et al.  CHROOCOCCOID CYANOBACTERIA IN LAKE ONTARIO: VERTICAL AND SEASONAL DISTRIBUTIONS DURING 1982 1 , 1985 .

[8]  H. Ducklow,et al.  The growth of heterotrophic bacteria in the surface waters of warm core rings , 1985 .

[9]  Allan Konopka,et al.  Seasonal Bacterial Production in a Dimictic Lake as Measured by Increases in Cell Numbers and Thymidine Incorporation , 1985, Applied and environmental microbiology.

[10]  J. Kuparinen,et al.  Assessing Phytoplankton and Bacterioplankton Production During Early Spring in Lake Erken, Sweden , 1984, Applied and environmental microbiology.

[11]  G. Bratbak,et al.  Bacterial dry matter content and biomass estimations , 1984, Applied and environmental microbiology.

[12]  G. Billen,et al.  Activity of heterotrophic bacteria and its coupling to primary production during the spring phytoplankton bloom in the southern bight of the North Sea , 1984 .

[13]  J. Fuhrman,et al.  Do Bacteria-Sized Marine Eukaryotes Consume Significant Bacterial Production? , 1984, Science.

[14]  T. D. Brock,et al.  Significance of Algal Excretory Products for Growth of Epilimnetic Bacteria , 1984, Applied and environmental microbiology.

[15]  M. Søndergaard,et al.  Measurements of Diel Rates of Bacterial Secondary Production in Aquatic Environments , 1984, Applied and environmental microbiology.

[16]  M. Fasham.  Flows of Energy and Materials in Marine Ecosystems: Theory and Practice , 1984 .

[17]  G. Likens,et al.  Decomposition of planktonic algae in an oligotrophic lake , 1984 .

[18]  B. Riemann.  Biomass and production of phyto‐ and bacterio‐plankton in eutrophic Lake Tystrup, Denmark , 1983 .

[19]  G. Ahlgren,et al.  Estimating Bacterioplankton Production by Measuring [3H]thymidine Incorporation in a Eutrophic Swedish Lake , 1983, Applied and environmental microbiology.

[20]  D. Kirchman,et al.  Estimates of bacterial growth from changes in uptake rates and biomass , 1982, Applied and environmental microbiology.

[21]  D. Strayer,et al.  Photosynthetically produced dissolved organic carbon: An important carbon source for planktonic bacteria1 , 1982 .

[22]  C. Pedrós-Alió,et al.  Assessing Biomass and Production of Bacteria in Eutrophic Lake Mendota, Wisconsin , 1982, Applied and environmental microbiology.

[23]  S. Y. Newell,et al.  Frequency of Dividing Cells as an Estimator of Bacterial Productivity , 1981, Applied and environmental microbiology.

[24]  R. Chambers,et al.  Nepheloid and suspended particulate matter in south-eastern Lake Michigan* , 1981 .

[25]  Wayne S Gardner,et al.  Intracellular Composition and Net Release Rates of Free Amino Acids in Daphnia magna , 1981 .

[26]  J. Fuhrman,et al.  Bacterioplankton Secondary Production Estimates for Coastal Waters of British Columbia, Antarctica, and California , 1980, Applied and environmental microbiology.

[27]  G. Harris.  Photosynthesis, productivity and growth , 1978 .

[28]  J. Hobbie,et al.  Daphnia grazing on natural bacteria1 , 1978 .

[29]  P. Calow,et al.  CONVERSION EFFICIENCIES IN HETEROTROPHIC ORGANISMS , 1977 .

[30]  J. Hobbie,et al.  Use of nuclepore filters for counting bacteria by fluorescence microscopy , 1977, Applied and environmental microbiology.

[31]  Woods,et al.  Determination of bacterial number and biomass in the marine environment , 1977, Applied and environmental microbiology.

[32]  E. Fee.  A Numerical Model for Determining Integral Primary Production and its Application to Lake Michigan , 1973 .

[33]  M. L. Watson.  Staining of Tissue Sections for Electron Microscopy with Heavy Metals , 1958, The Journal of biophysical and biochemical cytology.

[34]  G. Fahnenstiel,et al.  Algal Organic Carbon Excretion in Lake Michigan , 1986 .

[35]  E. Stoermer,et al.  Importance of Picoplankton in Lake Superior , 1986 .

[36]  T. Fenchel.  The ecology of heterotrophic microflagellates , 1986 .

[37]  G. Fahnenstiel,et al.  Influence of Salmonine Predation and Weather on Long-Term Water Quality Trends in Lake Michigan , 1986 .

[38]  J. Fuhrman,et al.  Biological considerations in the measurement of dissolved free amino acids in seawater and implications for chemical and microbiological studies , 1985 .

[39]  R. Newell,et al.  The Biological Role of Detritus in the Marine Environment , 1984 .

[40]  Å. Hagström.  Aquatic bacteria: Measurements and significance of growth , 1984 .

[41]  Wayne S Gardner,et al.  Sediment Trap Studies in Lake Michigan: Resuspension and Chemical Fluxes in the Southern Basin , 1984 .

[42]  R. T. Wright,et al.  Dynamics of Pools of Dissolved Organic Carbon , 1984 .

[43]  T. Fenchel.  Suspended Marine Bacteria as a Food Source , 1984 .

[44]  J. Hobbie,et al.  Heterotrophic activity in the sea , 1984 .

[45]  J. Sieburth.  Protozoan Bacterivory in Pelagic Marine Waters , 1984 .

[46]  J. Sharp.  Inputs into Microbial Food Chains , 1984 .

[47]  J. G. Field,et al.  The Ecological Role of Water-Column Microbes in the Sea* , 1983 .

[48]  Simmons,et al.  Physical behavior of PCBs in the Great Lakes , 1983 .

[49]  T. Fenchel.  Ecology of heterotrophic microflagellates. II Bioenergetics and growth , 1982 .

[50]  R. Chambers,et al.  Nearshore chemistry in the vicinity of the Grand River, Michigan , 1980 .

[51]  D. Lazinsky,et al.  PARAFORMALDEHYDE -GLUTARALDEHYDE AS A ROUTINE PHYTOPLANKTON FIXATIVE , 1979 .

[52]  R. Chróst.  The estimation of extracellular release by phytoplankton and heterotrophic activity of aquatic bacteria. , 1978, Acta microbiologica Polonica.

[53]  M. Jordán,et al.  Improved filtration procedures for freshwater [35S]SO4 uptake studies 1 , 1978 .

[54]  Chróst Rj.  The estimation of extracellular release by phytoplankton and heterotrophic activity of aquatic bacteria. , 1978 .

[55]  Graham P. Harris,et al.  Photosynthesis, productivity and growth, the physiological ecology of phytoplankton , 1978 .

[56]  T. Parsons,et al.  A practical handbook of seawater analysis , 1968 .