Application of Flow Programming in the Analysis of Drugs and Their Metabolites in Biological Fluids

Abstract An HPLC procedure using flow programming under isocratic elution conditions for determination of drugs and their metabolites in biological fluids is discussed. This technique was used in the analysis of triamterene and its metabolites in urine, chlorothiazide in plasma and hydrochlorothiazide in urine. Advantages of flow programming over conventional procedures such as isocratic elution and gradient elution are discussed.