Fourier-transform spectroscopy combined with a 5-fs broadband pulse for multispectral nonlinear microscopy

We propose a technique for distinguishing the origin of a four-wave mixing (FWM) signal. This technique is based on nonlinear Fourier-transform spectroscopy (FTS) combined with the use of a broadband pulse. We theoretically analyze FWM-FTS and show that the response function in FWM is obtained from the FWM power spectrum calculated by the Fourier transform of an interferometric autocorrelation signal. When a broadband pulse is employed as an excitation light source, the FWM power spectrum shows not only Raman resonance but also two-photon electronic resonance. By comparing the FWM power spectrum of a resonant sample with that of a nonresonant reference sample, the origin of FWM is identified. By employing theoretical analysis, we experimentally demonstrate FWM-FTS using a 5-fs broadband pulse. By combining the use of a 5-fs pulse with nonlinear FTS based on two-photon excited fluorescence, we also successfully measured a two-photon excitation spectrum with a bandwidth of 300 nm. Laser Quantum Product VENTEON PULSE ONE ULTRABROAD Institute 1Laser Technology Laboratory, RIKEN 2-1 Hirosawa, Wako, Saitama 351-0198, Japan 2Department of Electronics and Electrical Engineering, Keio University, Yokohama 223-8522, Japan 3Laboratory for Cell Function Dynamics, Brain Science Institute, Wako, Saitama 351-0198, Japan