Achromatized endomicroscope objective for optical biopsy

Currently, researchers and clinicians lack achromatized endomicroscope objectives that are as narrow as biopsy needles. We present a proof-of-concept prototype that validates the optical design of an NA0.4 objective. The objective, built with plastic lenses, has a 0.9 mm clear aperture and is achromatized from 452 nm to 623 nm. The objective’s measured Strehl ratio is 0.74 ± 0.05 across a 250 μm FOV. We perform optical sectioning via structured illumination through the objective while capturing fluorescence images of breast carcinoma cells stained with proflavine and cresyl violet. This technology has the potential to improve optical biopsies and provide the next step forward in cancer diagnostics.

[1]  Timothy J Muldoon,et al.  High-resolution imaging in Barrett's esophagus: a novel, low-cost endoscopic microscope. , 2008, Gastrointestinal endoscopy.

[2]  Angelique Kano,et al.  Design and demonstration of a miniature catheter for a confocal microendoscope. , 2004, Applied optics.

[3]  R. Gordon,et al.  Development of a versatile two-photon endoscope for biological imaging , 2010, Biomedical optics express.

[4]  Rebecca Richards-Kortum,et al.  Toward a low-cost compact array microscopy platform for detection of tuberculosis. , 2011, Tuberculosis.

[5]  Rebecca R. Richards-Kortum,et al.  Fiber-optic confocal reflectance microscope with miniature objective for in vivo imaging of human tissues , 2002, IEEE Transactions on Biomedical Engineering.

[6]  László Tabár,et al.  The role of radiological–pathological correlation in diagnosing early breast cancer: the pathologist’s perspective , 2011, Virchows Archiv.

[7]  Tomasz S. Tkaczyk,et al.  Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy , 2010, Optics express.

[8]  M. Levene,et al.  Microprisms for in vivo multilayer cortical imaging. , 2009, Journal of neurophysiology.

[9]  A. Zvyagin Multiphoton endoscopy , 2007 .

[10]  H Bartels,et al.  Quantitative Study of Breast Cancer Progression: Different Pathways for Various In Situ Cancers , 2002, Modern Pathology.

[11]  W. Drexler,et al.  In vivo ultrahigh-resolution optical coherence tomography of mouse colon with an achromatized endoscope. , 2006, Journal of biomedical optics.

[12]  Matthew D. Chidley,et al.  Design, assembly, and optical bench testing of a high-numerical-aperture miniature injection-molded objective for fiber-optic confocal reflectance microscopy. , 2006, Applied optics.

[13]  Tim N. Ford,et al.  Fluorescence endomicroscopy with structured illumination. , 2008, Optics express.

[14]  Xingde Li,et al.  Super-Achromatic Rapid Scanning Microendoscope for Ultrahigh-Resolution OCT Imaging , 2007, IEEE Journal of Selected Topics in Quantum Electronics.

[15]  Tomasz S. Tkaczyk,et al.  Ultra-slim plastic endomicroscope objective for non-linear microscopy , 2011, Optics express.

[16]  R Prescott,et al.  Correction of chromatic aberrations in GRIN endoscopes. , 1983, Applied optics.

[17]  M. Rajadhyaksha,et al.  Confocal mosaicing microscopy of human skin ex vivo: spectral analysis for digital staining to simulate histology-like appearance. , 2011, Journal of biomedical optics.

[18]  Xingde Li,et al.  Fiber-optic scanning two-photon fluorescence endoscope. , 2006, Optics letters.

[19]  Paul J. van Diest,et al.  Nuclear morphometric features in benign breast tissue and risk of subsequent breast cancer , 2006, Breast Cancer Research and Treatment.

[20]  Tomasz S Tkaczyk,et al.  Hyperspectral Shack-Hartmann test. , 2010, Applied optics.

[21]  Xingde Li,et al.  Combined influences of chromatic aberration and scattering in depth-resolved two-photon fluorescence endospectroscopy , 2010, Biomedical optics express.

[22]  Daniel S Gareau,et al.  Feasibility of digitally stained multimodal confocal mosaics to simulate histopathology. , 2009, Journal of biomedical optics.

[23]  E. Vicaut,et al.  Fibered Confocal Fluorescence Microscopy (Cell-viZio™) Facilitates Extended Imaging in the Field of Microcirculation , 2004, Journal of Vascular Research.

[24]  Timothy J Muldoon,et al.  Subcellular-resolution molecular imaging within living tissue by fiber microendoscopy. , 2007, Optics express.

[25]  Robert T Kester,et al.  Low cost, high performance, self-aligning miniature optical systems. , 2009, Applied optics.

[26]  Francois Lacombe,et al.  In vivo fibered confocal reflectance imaging: totally non-invasive morphological cellular imaging brought to the endoscopist , 2006, SPIE BiOS.

[27]  Stefan Bäumer Handbook of plastic optics , 2005 .

[28]  Tomasz S. Tkaczyk,et al.  Multi-modal miniature microscope: 4M Device for bio-imaging applications - an overview of the system , 2005, SPIE Optics + Optoelectronics.

[29]  Jochen Herms,et al.  Optical needle endoscope for safe and precise stereotactically guided biopsy sampling in neurosurgery. , 2012, Optics express.

[30]  Charles P. Lin,et al.  In vivo confocal and multiphoton microendoscopy. , 2008, Journal of biomedical optics.

[31]  Timothy J Muldoon,et al.  Evaluation of quantitative image analysis criteria for the high-resolution microendoscopic detection of neoplasia in Barrett's esophagus. , 2010, Journal of biomedical optics.

[32]  E. G. Ezhov,et al.  Design of achromatic and apochromatic plastic micro-objectives. , 2010, Applied optics.

[33]  A. Mehta,et al.  Multiphoton endoscopy: optical design and application to in vivo imaging of mammalian hippocampal neurons , 2003, Conference on Lasers and Electro-Optics, 2003. CLEO '03..