dsRNAs Targeted to the Brown Planthopper Nilaparvata lugens: Assessing Risk to a Non-Target, Beneficial Predator, Cyrtorhinus lividipennis.

RNA interference (RNAi) technology is becoming a maturing insect management approach. Before commercial-scale application, however, it is necessary to assess risks to non-target organisms (NTOs). Here, we evaluated the influence of RNAi technology, targeted to the brown planthopper (BPH, Nilaparvata lugens, Hemiptera: Delphacidae), a serious pest of Asian rice cropping systems, by dsRNA feeding. Three dsRNA fragments, targeting sodium channel protein Nach-like (dsNlNa), autophagy protein 5 (dsNlAup5), and V-type proton ATPase catalytic subunit A (dsNlvATP-A), which were highly lethal to BPH, were selected to evaluate their effects on an important predator of BPH, Cyrtorhinus lividipennis (Hemiptera: Miridae). It showed that these three dsRNA fragments posed no risks to C. lividipennis at worst-case treatments when fed with high concentrations (10×) dsRNAs. These findings not only establish part of a risk assessment protocol for RNAi-based products on NTOs but also contribute to the development and deployment of new technologies for BPH management.

[1]  X. Zhou,et al.  Oral RNAi toxicity assay suggests clathrin heavy chain as a promising molecular target for controlling the 28-spotted potato ladybird, Henosepilachna vigintioctopunctata. , 2021, Pest management science.

[2]  S. Bradbury,et al.  Evaluating toxicity of Varroa mite (Varroa destructor)-active dsRNA to monarch butterfly (Danaus plexippus) larvae , 2021, PloS one.

[3]  Hao Chen,et al.  Consumption of miRNA-Mediated Insect-Resistant Transgenic Rice Pollen Does Not Harm Apis mellifera Adults. , 2021, Journal of agricultural and food chemistry.

[4]  Zhaojun Han,et al.  Off-target effects of RNAi correlate with the mismatch rate between dsRNA and non-target mRNA , 2021, RNA biology.

[5]  Jie Shen,et al.  Nanoparticle‐mediated double‐stranded RNA delivery system: A promising approach for sustainable pest management , 2020, Insect science.

[6]  X. Zhou,et al.  Dietary RNAi toxicity assay exhibits differential responses to ingested dsRNAs among lady beetles. , 2020, Pest management science.

[7]  Y. Devos,et al.  Risk Assessment Considerations for Genetically Modified RNAi Plants: EFSA’s Activities and Perspective , 2020, Frontiers in Plant Science.

[8]  Jie Shen,et al.  A novel plasmid-Escherichia coli system produces large batch dsRNAs for insect gene silencing. , 2020, Pest management science.

[9]  Neena Mitter,et al.  A Perspective on RNAi-Based Biopesticides , 2020, Frontiers in Plant Science.

[10]  C. Coustau,et al.  RNA-based technologies for insect control in plant production. , 2020, Biotechnology advances.

[11]  S. R. Palli,et al.  Mechanisms, Applications, and Challenges of Insect RNA Interference. , 2020, Annual review of entomology.

[12]  B. Siegfried,et al.  Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi. , 2019, Pest management science.

[13]  Meizhen Yin,et al.  Spray method application of transdermal dsRNA delivery system for efficient gene silencing and pest control on soybean aphid Aphis glycines , 2019, Journal of Pest Science.

[14]  F. Wang,et al.  Cry2A rice did not affect the interspecific interactions between two rice planthoppers, Nilaparvata lugens, and Sogatella furcifera. , 2019, GM crops & food.

[15]  Wenwu Zhou,et al.  Identification and expression analysis of putative chemoreception genes from Cyrtorhinus lividipennis (Hemiptera: Miridae) antennal transcriptome , 2018, Scientific Reports.

[16]  O. Christiaens,et al.  RNA interference technology in crop protection against arthropod pests, pathogens and nematodes. , 2018, Pest management science.

[17]  Ronald D. Flannagan,et al.  Evaluation of SmartStax and SmartStax PRO maize against western corn rootworm and northern corn rootworm: efficacy and resistance management. , 2017, Pest management science.

[18]  H. Hua,et al.  Bt rice in China — focusing the nontarget risk assessment , 2017, Plant biotechnology journal.

[19]  T. Zhu,et al.  Ras-like family small GTPases genes in Nilaparvata lugens: Identification, phylogenetic analysis, gene expression and function in nymphal development , 2017, PloS one.

[20]  G. Jander,et al.  A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters , 2016, Scientific Reports.

[21]  Huipeng Pan,et al.  Assessment of Potential Risks of Dietary RNAi to a Soil Micro-arthropod, Sinella curviseta Brook (Collembola: Entomobryidae) , 2016, Front. Plant Sci..

[22]  Xiaojun Liu,et al.  Functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference , 2016, Scientific Reports.

[23]  Sudhir Kumar,et al.  MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. , 2016, Molecular biology and evolution.

[24]  Xuguo Zhou,et al.  Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L. , 2016, Chemosphere.

[25]  M. Stanke,et al.  Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target , 2015, BMC Genomics.

[26]  R. Bock,et al.  Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids , 2015, Science.

[27]  Guy Smagghe,et al.  The challenge of RNAi-mediated control of hemipterans. , 2014, Current opinion in insect science.

[28]  H. Tian,et al.  The Insect Ecdysone Receptor is a Good Potential Target for RNAi-based Pest Control , 2014, International journal of biological sciences.

[29]  J. Romeis,et al.  Bt rice producing Cry1C protein does not have direct detrimental effects on the green lacewing Chrysoperla sinica (Tjeder) , 2014, Environmental toxicology and chemistry.

[30]  Xinda Lin,et al.  Characterization of the Distal-less gene homologue, NlDll, in the brown planthopper, Nilaparvata lugens (Stål). , 2014, Gene.

[31]  B. Wiggins,et al.  Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) , 2013, Transgenic Research.

[32]  Lili Zhu,et al.  Knockdown of Midgut Genes by dsRNA-Transgenic Plant-Mediated RNA Interference in the Hemipteran Insect Nilaparvata lugens , 2011, PloS one.

[33]  V. Beneš,et al.  The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. , 2009, Clinical chemistry.

[34]  Daniel R. G. Price,et al.  RNAi-mediated crop protection against insects. , 2008, Trends in biotechnology.

[35]  Geert Plaetinck,et al.  Control of coleopteran insect pests through RNA interference , 2007, Nature Biotechnology.

[36]  Anastasia Khvorova,et al.  Off-target effects by siRNA can induce toxic phenotype. , 2006, RNA.

[37]  Tomoyuki Yamada,et al.  dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference , 2005, Nucleic Acids Res..

[38]  Gregory J. Hannon,et al.  Insight Review Articles , 2022 .

[39]  Zong-xiu Sun,et al.  A chemically defined diet enables continuous rearing of the brown planthopper, Nilaparvata lugens (Stål) (Homoptera: Delphacidae) , 2001 .

[40]  T. Chua,et al.  Effects of Prey Number and Stage on the Biology of Cyrtorhinus lividipennis (Hemiptera: Miridae): A Predator of Nilaparvata lagens (Homoptera: Delphacidae) , 1989 .