Determination of ATP via the photochemical generation of hydrogen peroxide using flow injection luminol chemiluminescence detection

The determination of ATP using the coupling between a photochemical reaction and a chemiluminescence reaction in a flow injection (FI) system is described. The method is based on the reaction of glucose with ATP catalyzed by hexokinase and Mg2+ ions. The glucose that is not consumed by ATP is subsequently photooxidized using 9,10-anthraquinone-2,6-disulfonate as a sensitizer. The hydrogen peroxide produced in the photochemical reaction is monitored through the chemiluminescence reaction with luminol catalyzed by hematine. There is a linear relationship between the decrease in the chemiluminescence response and the ATP concentration at a constant concentration of glucose. Under the optimum conditions, the calibration graph is linear in the range 0.20–50.5 mg L−1 with a throughput of 25 samples per hour and relative standard deviations between ±0.62 and ±1.42%. The limit of detection is 0.07 mg L−1. The method was used for the determination of ATP in pharmaceuticals, milk, and soils.

[1]  Elo Harald Hansen,et al.  Flow-injection bioluminescent determination of ATP based on the use of the luciferin-luciferase system , 1994 .

[2]  D. Adams,et al.  Evidence that ischemic cell death begins in the subendocardium independent of variations in collateral flow or wall tension. , 1983, Circulation.

[3]  V. Tomás,et al.  Flow injection determination of lactate based on a photochemical reaction using photometric and chemiluminescence detection. , 1999, The Analyst.

[4]  Bo Olsson,et al.  Determination of hydrogen peroxide in a flow system with microperoxidase as catalyst for the luminol chemiluminescence reaction , 1982 .

[5]  A. Roda,et al.  Bioluminescent continuous-flow assay of adenosine 5'-triphosphate using firefly luciferase immobilized on nylon tubes. , 1986, Analytical Chemistry.

[6]  John M. Ryder,et al.  Determination of adenosine triphosphate and its breakdown products in fish muscle by high performance liquid chromatography , 1985 .

[7]  W. Verstraete,et al.  ATP content of soils estimated by two contrasting extraction methods , 1983 .

[8]  T. Pérez-Ruíz,et al.  Flow-injection chemiluminometric determination of ascorbic acid based on its sensitized photooxidation , 1995 .

[9]  T. Kumamaru,et al.  Coupling of membrane-immobilized enzyme reaction and heteropoly acid-luminol chemiluminescence reaction for the determination of adenosine-5'-triphosphate , 1997 .

[10]  J. Lowe,et al.  Simple step gradient elution of the major high-energy compounds and their catabolites in cardiac muscle using high-performance liquid chromatography. , 1986, Journal of chromatography.

[11]  S. S. Chen,et al.  Rapid analysis of adenosine, AMP, ADP, and ATP by anion-exchange column chromatography. , 1984, Journal of chromatography.

[12]  F. Korte,et al.  A simple effective procedure for the determination of adenosine triphosphate in soils , 1988 .

[13]  J. Oades,et al.  A method for measuring adenosine triphosphate in soil , 1979 .

[14]  D. E. Atkinson.  Cellular Energy Metabolism and its Regulation , 1977 .

[15]  G. K. Bedford,et al.  High-performance liquid chromatographic method for the simultaneous determination of myocardial creatine phosphate and adenosine nucleotides. , 1984, Journal of chromatography.

[16]  James N. Miller,et al.  Detection of bacterial ATP by reversed flow-injection analysis with luminescence detection , 1992 .

[17]  J. Birks,et al.  Photoinitiation of peroxyoxalate chemiluminescence: application to flow injection analysis of chemilumophores , 1990 .

[18]  S. Trajkovska,et al.  Determination of validity for blood for transfusion by firefly bioluminescent assay for ATP , 1994 .

[19]  Juan L. Silva,et al.  Aerobic Counts, Color and Adenine Nucleotide Changes in CO2 Packed Refrigerated Striped Bass Strips , 1994 .

[20]  W. Seitz,et al.  Improved determination of hydrogen peroxide by measurement of peroxyoxalate chemiluminescence , 1980 .

[21]  Hans Ulrich Bergmeyer,et al.  Methods of Enzymatic Analysis , 2019 .

[22]  J. Burr.  Chemi- and Bioluminescence , 1985 .

[23]  J. Birks,et al.  Photooxygenation-chemiluminescence high-performance liquid chromatographic detector for the determination of aliphatic alcohols, aldehydes, ethers and saccharides , 1982 .

[24]  F. Korte,et al.  Atp-measurements in soil: A combination between the TCA and NRB ® extraction methods , 1985 .

[25]  V. Tomás,et al.  Flow-injection chemiluminometric determination of citrate based on a photochemical reaction , 1995 .

[26]  Y. Umezawa,et al.  Na+,K+-ATPase-based bilayer lipid membrane sensor for adenosine 5′-triphosphate , 1993 .

[27]  G. Christian,et al.  Electrochemical study of the degradation of vitamin K3 and vitamin K3 bisulfite , 1979 .

[28]  V. Stocchi,et al.  Fast reversed-phase high-performance liquid chromatographic determination of nucleotides in red blood cells. , 1984, Journal of chromatography.

[29]  K. Yamanaka,et al.  Potentiometric method for the determination of adenosine-5′-triphosphate , 1993 .

[30]  Paul J. Worsfold,et al.  The bioluminescent determination of adenosine triphosphate with a flow-injection system , 1985 .

[31]  G. Pacey,et al.  Determination of ATP using chelation-enhanced fluorescence. , 1996, Talanta.

[32]  G. Guilbault,et al.  Glucose oxidase/hexokinase electrode for the determination of ATP , 1997 .

[33]  V. Tomás,et al.  Flow-injection fluorimetric determination of vitamin K(1) based on a photochemical reaction. , 1999, Talanta.